INTRARECTAL TITRATION OF SHIV 162P4 STOCK

SHIV 162P4 库存的直肠内滴定

• 批准号: 8357586
• 负责人: Shiu-Lok Hu
• 金额: $ 32.86万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8357586
• 关键词: Animals; Antibodies; Antibody Formation; CCR5 gene; Comparative Study; Data; Epitopes; Evolution; Family suidae; Funding; Goals; Grant; HIV-1; Infection; Macaca; Macaca mulatta; Molecular Conformation; Monitor; National Center for Research Resources; Pathogenicity; Peripheral Blood Mononuclear Cell; Primates; Principal Investigator; Reporting; Research; Research Infrastructure; Resources; Route; Source; Tail; Titrations; United States National Institutes of Health; Universities; Viral; Virus Diseases; cost; in vivo; neutralizing antibody; neutralizing monoclonal antibodies; simian human immunodeficiency virus

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. We previously reported a comparative study of the in vivo infectivity and pathogenicity of a CCR5-tropic SHIVSF162 P4 in rhesus and pig-tailed macaques. Results indicate that both rhesus and pig-tailed macaques are similarly susceptible to SHIVSF162 P4 infection by mucosal routes. However, SHIV replication was significantly more robust in pig-tailed macaques than in rhesus. Since, persistent viral infection has been correlated with broadening of neutralizing antibody responses, we continued to monitor the evolution of viral sequences and neutralizing antibody activities in a subset of infected pig-tailed macaques after the primary goal of this study was achieved. PBMC from some of these animals were sent to Dr. James Robinson at Tulane University to isolate neutralizing monoclonal antibodies against HIV-1. Preliminary data from Dr. Robinson indicate that some of these antibodies may recognize conformation-dependent epitopes against HIV-1 envelope. Further characterization of these antibodies is in progress.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 我们此前曾报道过对恒河猴和猪尾猕猴中CCR5-循环SHIVSF162 P4的体内感染性和致病性的比较研究。结果表明，恒河猴和猪尾猕猴同样容易受到粘膜途径的Shivsf162 P4感染。然而，猪尾猕猴的湿夫复制比在恒河猴中更强大。由于，持续的病毒感染与中和中和抗体反应的扩展相关，因此在实现了这项研究的主要目标之后，我们继续监测病毒序列的演变和中和抗体活性的演变。其中一些动物的PBMC被送往杜兰大学的詹姆斯·罗宾逊博士，以隔离中和对HIV-1的单克隆抗体。罗宾逊博士的初步数据表明，其中一些抗体可能识别依赖于HIV-1包膜的构象的表位。这些抗体的进一步表征正在进行中。