FUNCTIONAL ORGANIZATION OF CERVICAL SPINAL INTERNEURONS

颈椎中间神经元的功能组织

• 批准号: 8172770
• 负责人: Steve I Perlmutter
• 金额: $ 15.51万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8172770
• 关键词: Affect; Cervical; Computer Retrieval of Information on Scientific Projects Database; Data; Data Collection; Electrodes; Exhibits; Funding; Grant; Institution; Interneurons; Iontophoresis; Methysergide; Monkeys; Movement; Neurons; Play; Property; Research; Research Personnel; Resources; Role; Serotonin; Serotonin Antagonists; Source; Spinal; Spinal Cord; United States National Institutes of Health; Wrist; extracellular

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Data collection was completed for our study of the effects of serotonin on the firing properties of neurons in the spinal cord of behaving monkeys. New data confirm our findings in other monkeys from previous years. Serotonin and the serotonin receptor antagonist methysergide were applied to extracellularly recorded neurons with a combined extracellular, iontophoresis electrode. Serotonin causes overall increase in activity of a significant percentage of spinal neurons. A small percentage exhibit decreased firing after iontophoresis of serotonin. Methysergide has the opposite effect for most of these neurons. The remaining neurons are not affected by either serotonin or its antagonist. The directional tuning of neurons in the C6-T1 segments was determined for multi-dimensional wrist movements before and after the iontophoretic application of serotonin and serotonin antagonists. Block of inhibition had little effect on the neurons' directional tuning for the majority of neurons. These results suggest that serotonin plays an important role in regulating excitability for many spinal neurons but does not affect spatial tuning for wrist movements in different directions.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 我们研究血清素对行为猴脊髓神经元放电特性的影响的数据收集已经完成。 新数据证实了我们前几年在其他猴子身上的发现。 使用组合的细胞外离子电渗电极将血清素和血清素受体拮抗剂美西麦角应用于细胞外记录的神经元。 血清素导致相当一部分脊髓神经元的活动总体增加。 一小部分人在血清素离子电渗疗法后表现出放电减少。 美西麦角对大多数神经元具有相反的作用。 其余的神经元不受血清素或其拮抗剂的影响。 在应用血清素和血清素拮抗剂之前和之后，针对多维手腕运动确定了 C6-T1 节段中神经元的定向调节。 对于大多数神经元来说，抑制阻滞对神经元的定向调节影响不大。 这些结果表明，血清素在调节许多脊髓神经元的兴奋性方面发挥着重要作用，但不影响不同方向手腕运动的空间调谐。