NIDCR DIR Scientific Cores

NIDCR DIR科学核心为进行牙科和颅面疾病研究的校内调查人员提供研究支持。基因靶向设施（GTF）允许研究人员在小鼠中“添加”或“减去”基因，以建立相关人类疾病的动物模型进行研究，并发现特定基因对宿主生理的影响。GTF产生嵌合小鼠，导致感兴趣的基因在种系传播。此外，还创建和/或重新建立了新的基因敲除小鼠和转基因小鼠。小鼠胚胎干细胞服务成功完成基因靶向实验。GTF还通过低温保存小鼠动物模型胚胎提供了重要的科学支持服务。这些服务促成了200多份研究和评论出版物（在ZIA项目中报告）。目前正在测试其他服务，包括精子和卵巢冷冻保存以及小鼠模型的体外受精服务，以及开发额外的小鼠胚胎干细胞系。DNA测序设备为NIDCR DIR研究者领导的研究提供了超过15,000个反应。我们目前正在将我们的流式细胞术、细胞分选、洗脱和激光捕获显微镜功能转移到核心设施。

The NIDCR DIR Scientific Cores provided research support for intramural investigators performing studies on dental and craniofacial diseases. The Gene Targeting Facility (GTF) allows investigators to "add" or "subtract" genes in mice to develop animal models of relevant human diseases for research and to discover the affect of specific genes on host physiology. The GTF generated chimeric mice resulting in germline transmission of the genes of interest. In addition new gene knockout and transgenic mice were created and/or re-established. The murine embryonic stem cell service successfully completed gene targeting experiments. The GTF also provided an important scientific support service by cryopreserving embryos from murine animal models. These services contributed to over 200 research and review publications (reported in ZIA projects). Testing is underway for additional services, including sperm and ovary cryopreservation and IVF services for murine models and development of additional murine embryonic stem cell lines. The DNA Sequencing Facility provided over 15,000 reactions for NIDCR DIR investigator-led research. We are currently in the process of moving our Flow Cytometry, Cell Sorting, Elutriation and Laser Capture Microscopy functions into the Core Facility.