NIDCR DIR Scientific Cores

NIDCR DIR科学核心为内部调查人员进行牙科和颅面疾病研究提供了研究支持。基因打靶设备(GTF)允许研究人员对小鼠的基因进行加法或减法，以开发用于研究的相关人类疾病的动物模型，并发现特定基因对宿主生理的影响。GTF产生了嵌合小鼠，导致了目的基因的生殖系传播。此外，还创造和/或重新建立了新的基因敲除和转基因小鼠。小鼠胚胎干细胞服务部成功完成基因打靶实验。GTF还通过冷冻保存小鼠动物模型的胚胎提供了重要的科学支持服务。这些服务为200多份研究和审查出版物(在ZIA项目中报告)作出了贡献。其他服务的测试正在进行中，包括精子和卵巢冷冻保存和小鼠模型的试管受精服务，以及开发更多的小鼠胚胎干细胞系。DNA测序设备为NIDCR DIR研究员领导的研究提供了15,000多个反应。

The NIDCR DIR Scientific Cores provided research support for intramural investigators performing studies on dental and craniofacial diseases. The Gene Targeting Facility (GTF) allows investigators to "add" or "subtract" genes in mice to develop animal models of relevant human diseases for research and to discover the affect of specific genes on host physiology. The GTF generated chimeric mice resulting in germline transmission of the genes of interest. In addition new gene knockout and transgenic mice were created and/or re-established. The murine embryonic stem cell service successfully completed gene targeting experiments. The GTF also provided an important scientific support service by cryopreserving embryos from murine animal models. These services contributed to over 200 research and review publications (reported in ZIA projects). Testing is underway for additional services, including sperm and ovary cryopreservation and IVF services for murine models and development of additional murine embryonic stem cell lines. The DNA Sequencing Facility provided over 15,000 reactions for NIDCR DIR investigator-led research.