NIDCR DIR Scientific Cores

NIDCR DIR科学核心为进行牙科和颅面疾病研究的校内调查人员提供研究支持。基因靶向核心（GTC）允许研究人员在小鼠中“添加”或“减去”基因，以建立相关人类疾病的动物模型进行研究，并发现特定基因对宿主生理的影响。GTC产生嵌合小鼠，导致感兴趣的基因在种系传播。此外，还创建和/或重新建立了新的基因敲除小鼠和转基因小鼠。小鼠胚胎干细胞服务成功完成基因靶向实验。GTC还通过低温保存小鼠胚胎和精子提供了重要的科学支持服务。这些服务促成了100多个研究和评论出版物（在ZIA项目中报告）。正在测试其他服务，包括小鼠模型的体外受精服务和开发额外的小鼠胚胎干细胞系。合并技术研究核心的形成是为了将几个核心功能置于一个核心主管和一个核心主管之下，包括DNA测序，它为NIDCR DIR研究者领导的研究提供了一千多种反应，流式细胞术和细胞分选，细胞洗脱，激光捕获显微镜和组织学。

The NIDCR DIR Scientific Cores provided research support for intramural investigators performing studies on dental and craniofacial diseases. The Gene Targeting Core (GTC) allows investigators to "add" or "subtract" genes in mice to develop animal models of relevant human diseases for research and to discover the affect of specific genes on host physiology. The GTC generated chimeric mice resulting in germline transmission of the genes of interest. In addition new gene knockout and transgenic mice were created and/or re-established. The murine embryonic stem cell service successfully completed gene targeting experiments. The GTC also provided an important scientific support service by cryopreserving embryos and sperm from murine animal models. These services contributed to over 100 research and review publications (reported in ZIA projects). Testing is underway for additional services, including IVF services for murine models and development of additional murine embryonic stem cell lines. The Combined Technical Research Core was formed to put several core functions under one Core Chief and a Core Director, including DNA Sequencing, which provided over a thousand reactions for NIDCR DIR investigator-led research, Flow Cytometry and Cell Sorting, Cell Elutriation, Laser Capture Microscopy, and Histology.