Gene Targeting and Transgenic Facility

基因打靶和转基因设施

• 批准号: 8231142
• 负责人: MICHAEL J. WEBER
• 金额: $ 3.76万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-02-01 至 2017-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8231142
• 关键词: Area; Biological Preservation; Cancer Center; Cancer Center Support Grant; Cancer Model; Clinic; Cryopreservation; Derivation procedure; Development; Drug resistance; Embryo; Epigenetic Process; Event; Evolution; Gene Targeting; Genetic; Genetically Engineered Mouse; Goals; Immune system; Instruction; Knock-in Mouse; Knock-out; Laboratories; Link; Malignant Neoplasms; Mission; Molecular; Mouse Strains; Mus; Mutation; Neoplasm Metastasis; Positioning Attribute; Production; Property; Research; Research Personnel; Resource Sharing; Resources; Services; Targeted Research; Technology; Training; Transgenic Organisms; Universities; University of Virginia Cancer Center; Virginia; anticancer research; cancer cell; design; embryonic stem cell; genetic analysis; genetic technology; human cancer mouse model; model design; mouse model; reconstitution; tool; tumor; tumor progression

The University of Virginia Gene Targeting and Transgenic Facility (GTTF) is a Shared Resource that utilizes advanced transgenic and gene targeting technologies to efficiently produce and preserve genetically engineered mice for cancer model research for UVA Cancer Center investigators. The GTTF's mission is to support transgenic and gene targeting research endeavors, to provide efficient genetic technologies for germline and embryonic stem cell manipulations, and to serve as a resource for design, development and derivation of customized cancer-prone mouse strains. For the past five years, the GTTF has evolved into a unique shared resource that provides a range of integrated services covering five areas: (1) transgenic and knockout/knock-in mouse production, (2) gene targeting, (3) embryo cryopreservation/reconstitution, (4) mouse genetic analysis and (5) training. The GTTF is uniquely positioned to support cancer research. While cancer researchers have successfully studied the molecular and cellular properties of cancer cells propagated in culture, increasingly mouse models of cancer provide a more physiologically relevant opportunity to investigate the interplay of cancer cells, the tumor microenvironment, and the host immune system. Mouse models provide opportunities to study the evolution of cancer progression from first mutations to metastasis, and the unique opportunity to assess the genetic and epigenetic events correlated with acquisition of drug resistance. The overall goal of the GTTF is to provide the support with integrated service and training to make mouse model design, creation and preservation as seamless as possible for the benefit of researchers in the Cancer Center.

弗吉尼亚大学基因靶向和转基因设施（GTTF）是一个共享资源， 利用先进的转基因和基因靶向技术， 为UVA癌症中心研究人员进行癌症模型研究的工程小鼠。全球专题信托基金的使命是 支持转基因和基因靶向研究工作，提供有效的遗传技术， 生殖系和胚胎干细胞操作，并作为设计，开发和 定制的癌症易感小鼠品系的衍生。 在过去五年中，全球专题信托基金已发展成为一种独特的共享资源， 综合服务涵盖五个领域：（1）转基因和敲除/敲入小鼠生产，（2）基因 靶向，（3）胚胎冷冻保存/重建，（4）小鼠遗传分析和（5）培训。全球专题信托基金 在支持癌症研究方面有着独特的地位。虽然癌症研究人员已经成功地研究了 在培养物中增殖的癌细胞的分子和细胞特性，越来越多的小鼠癌症模型 为研究癌细胞、肿瘤细胞和肿瘤细胞之间的相互作用提供了一个更符合生理学的机会。 微环境和宿主免疫系统。小鼠模型提供了研究进化的机会 癌症从第一次突变到转移的进展，以及评估基因突变的独特机会。 以及与获得耐药性相关的表观遗传事件。全球专题信托基金的总体目标是 提供综合服务和培训支持，使鼠标模型设计，创作和 为了癌症中心的研究人员的利益，尽可能无缝地保存。