Biomolecular Analysis

生物分子分析

• 批准号: 8231134
• 负责人: MICHAEL J. WEBER
• 金额: $ 15.63万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-02-01 至 2017-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8231134
• 关键词: Area; Bioinformatics; Cancer Center; Cancer Center Support Grant; Complex Mixtures; DNA; DNA Sequence; Data Analyses; Ensure; Experimental Designs; Gene Expression; Genomics; Genotype; Informatics; Instruction; Malignant Neoplasms; Mass Spectrum Analysis; Molecular Analysis; National Center for Research Resources; Post-Translational Protein Processing; Proteins; Proteomics; Protocols documentation; Reagent; Recommendation; Research Project Grants; Resource Sharing; Resources; Science; Services; Time; Work; cost; insight; instrumentation; member

The Biomolecular Analysis Facility (BAF) provides a centralized setfing for a diverse suite of services, instrumentation, and expertise projected through two areas: DNA Sciences and Mass Spectrometry/Proteomics. Together, the staff of the BAF provides expertise to Cancer Center Members for experimental design, execufion, and data analysis. The scope of services provided includes DNA sequencing and genomic analyses carried out via Sanger and "next-gen" sequencing and gene expression and genotyping on microarray and real time PCR platforms; as well as protein identification, posttranslational modification analysis, complex mixture analysis and qualitative and quantitafive proteomics carried out via mass spectrometry. The consolidated organization of these technological areas not only allows for a seamless science flow from experimental genomics to proteomics but also allows for a collaborative synergy among the staff, with a sharing of resources and a mutual requirement for bioinformatic and informatic expertise and support. Further, this consolidation of services aligns with the recommendations from the National Center for Research Resources for core consolidation for the enhancement of service provision and cost-effecfiveness. Through the Cancer Center Execufive Committee and institufional Office of Resource Core Administration (ORCA) the BAF maintains a close working relationship with the Cancer Center and its members to ensure the services, protocols, reagents and expertise required are made available. In many instances these resources are specifically tailored to the needs of Cancer Center Members' research projects and grant applicafions.

生物分子分析设施（BAF）为各种服务提供集中设置， 仪器和专业知识预计通过两个领域：DNA科学和质量 光谱/蛋白质组学。总之，BAF的工作人员为癌症中心成员提供专业知识， 实验设计、执行和数据分析。提供的服务范围包括DNA 通过桑格和“下一代”测序和基因表达进行测序和基因组分析 以及在微阵列和真实的时间PCR平台上的基因分型;以及蛋白质鉴定、翻译后 修饰分析、复杂混合物分析和定性定量蛋白质组学 通过质谱法进行。这些技术领域的统一组织不仅 允许从实验基因组学到蛋白质组学的无缝科学流程， 工作人员之间的协作协同作用，资源共享和生物信息学的相互要求 信息专业知识和支持。此外，这种服务合并符合各项建议， 来自国家研究资源中心的核心整合，以增强服务 供应和成本效益。通过癌症中心执行委员会和机构办公室 资源核心管理局（ORCA）的BAF与癌症保持密切的工作关系， 中心及其成员确保提供所需的服务、方案、试剂和专业知识 available.在许多情况下，这些资源是专门针对癌症中心的需求量身定制的。 会员之研究计画及补助金申请。