生物化学、病理学和影像核心-猫眼课题宝

生物化学、病理学和影像核心

批准号：

8181496

负责人：

Ravichandran Ramasamy

金额：

$24.61万

依托单位：

NEW YORK UNIVERSITY SCHOOL OF MEDICINE

依托单位国家：

美国

项目类别：

财政年份：

资助国家：

美国

项目状态：

已结题

起止时间：

至 2016-11-30

来源：

https://reporter.nih.gov/project-details/8181496

关键词：

3-deoxyglucosone Acids Adopted Albumins Animals Antibodies Aorta Apoptosis Atherosclerosis Biochemical Biochemistry Biological Assay Biology Blood Vessels Buffers Cardiovascular Diseases Cells Critiques Cultured Cells Detection Diabetes Mellitus Diacetyl Disease Enzyme-Linked Immunosorbent Assay Epitopes Equipment Fibronectins Fluorescence Functional disorder Generations Glucose Glucose-6-Phosphate Glucosephosphates Glutathione Glutathione Disulfide Heart High Pressure Liquid Chromatography Homidium Bromide Hour Hydrogen Peroxide Image Imaging Techniques Immunoglobulin G Immunohistochemistry Incidence Incubated Individual Instruction Letters Link Lysine Manufacturer Name Measurement Measures Mediator of activation protein Messenger RNA Methods Mitochondria Modification Molecular Probes Monitor Mus Myocardial Infarction NADPH Oxidase Oryctolagus cuniculus Oxidative Stress Oxis Pathology Perchloric Acids Peripheral arterial disease Plant Roots Plasma Post-Translational Protein Processing Procedures Process Proteins Publications Publishing Pyruvaldehyde Reaction Reader Reading Recommendation Research Sampling Severities Signal Transduction Standardization Sterility Superoxides System Techniques Time Tissues Weight Western Blotting angiogenesis catalase diacetyldichlorofluorescein ethyl lysine experience fluorophore fructosyl-lysine hydroethidine in vivo methionine sulfoxide molecular imaging myocardial infarct sizing novel pentosidine programs receptor for advanced glycation endproducts response

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PROJECT SUMMARY (See instructions): Core B, the Biochemistry, Pathology and Imaging Core will continue to serve all three Projects through all five years of the Program. The core is composed of three units: (1) The Biochemistry Unit of Core B will perform assays on tissue/plasma of mice and cultured cells in the individual projects for assessment of oxidative stress, AGEs and other biochemical mediators linked to RAGE and vascular dysfunction. (2) The Pathology Unit of Core B will perform pathological analysis of mouse tissues. This Unit will serve all 3 projects for standardization of immunohistochemistry and semiquantitative analyses. The Unit will perform analysis of atherosclerosis (aortas (atherosclerosis at the aortic root and en face assessment of aorta), angiogenesis (Project 2-3) and myocardial infarction size (Project 3). (3) The newly-formed Imaging Unit will perform novel imaging techniques using state-of-the-art new equipment for molecular imaging studies in atherosclerosis and apoptosis (Project 1) and angiogenesis (Project 2).

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