Functional architecture of the auditory cortex

听觉皮层的功能结构

• 批准号: 8035153
• 负责人: CHARLES C LEE
• 金额: $ 5.06万
• 依托单位: UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-12-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8035153
• 关键词: Age; Aging; Anatomy; Architecture; Area; Auditory; Auditory Perception; Auditory Physiology; Auditory area; Auditory system; Diagnostic; Disease; Electric Stimulation; Electron Microscopy; Feedback; Fiber; Foundations; Glutamates; Goals; In Vitro; Injection of therapeutic agent; Investigation; Knowledge; Label; Lead; Methods; Midbrain structure; Morphology; Mus; Neurons; Output; Pathway interactions; Patients; Pattern; Physiological; Physiology; Preparation; Process; Property; Relative (related person); Research; Role; Route; Slice; Staining method; Stains; Synapses; Testing; Thalamic structure; Tracer; Virus; Weight; Whole-Cell Recordings; Work; base; behavior change; biocytin; improved; information processing; insight; nervous system disorder; novel; optical imaging; research study; response

DESCRIPTION (provided by applicant): The goals of this study will be to investigate the synaptic properties of auditory cortical synapses, in order to assess their putative roles in auditory information processing. The broad objectives of this research are to understand the functional contributions of these projections to auditory perception and behavior and how changes in these synaptic properties manifest with age and in disease processes. I have already identified several anatomical and physiological properties of glutamatergic synapses in the central auditory system, which forms the basis for the proposed investigation. This study will utilize an in vitro slice preparation in the mouse, which has been developed for the auditory cortical pathways. Whole-cell recordings in cortical neurons will be used to record responses to minimal electrical stimulation, photostimulation by uncaging of glutamate, and photostimulation of channelrhodopsin expressing fibers in order to characterize their topographic organization and physiological properties. In addition, a novel method of optical imaging of responses revealed by autofluorescence will be employed to help trace the output patterns of auditory cortical projections. Morphological correlates will be sought using intracellular injections of biocytin. The proposed experiments should lead to further insight on the information-bearing roles of the auditory cortical projections, as well as help to establish their relative weights in the organization of central processing in the auditory system. PUBLIC HEALTH RELEVANCE: This study should enhance our knowledge and perspective of the normal modes of information processing in the auditory system. It should provide a foundation for the further exploration of abnormal processing in auditory neurological diseases and aging, which will eventually lead to improved diagnostics and treatments for patients with disorders of the auditory system.

描述（由申请人提供）：本研究的目标是研究听觉皮质突触的突触特性，以评估其在听觉信息处理中的推定作用。这项研究的广泛目标是了解这些预测对听觉感知和行为的功能贡献，以及这些突触特性的变化如何与年龄和疾病过程中表现出来。我已经确定了中央听觉系统中谷氨酸能突触的几种解剖学和生理特性，这是拟议研究的基础。这项研究将利用小鼠的体外切片制剂，该切片是为听觉皮层途径开发的。皮质神经元中的全细胞记录将用于记录对最小电刺激的响应，通过谷氨酸的渗透刺激以及表达纤维的通道旋转蛋白的光刺激，以表征其地形组织和生理特性。此外，将采用自动荧光揭示的一种新型的光学成像方法，以帮助追踪听觉皮质投影的输出模式。将使用生物细胞内注射生物细胞的注射来寻求形态相关性。提出的实验应导致进一步了解听觉皮质预测的信息角色，并有助于在听觉系统中的中央处理组织中建立相对权重。 公共卫生相关性：这项研究应增强我们对听觉系统中信息处理方式的知识和观点。它应该为进一步探索听觉神经系统疾病和衰老异常处理的基础，最终导致改善听觉系统疾病患者的诊断和治疗方法。