Functional architecture of the auditory cortex

听觉皮层的功能结构

• 批准号: 8260890
• 负责人: CHARLES C LEE
• 金额: $ 10万
• 依托单位: LOUISIANA STATE UNIV A&M COL BATON ROUGE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-12-01 至 2013-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8260890
• 关键词: Age; Aging; Anatomy; Architecture; Area; Auditory; Auditory Perception; Auditory Physiology; Auditory area; Auditory system; Diagnostic; Disease; Electric Stimulation; Electron Microscopy; Feedback; Fiber; Foundations; Glutamates; Goals; Health; In Vitro; Injection of therapeutic agent; Investigation; Knowledge; Label; Lead; Methods; Midbrain structure; Morphology; Mus; Neurons; Output; Pathway interactions; Patients; Pattern; Physiological; Physiology; Preparation; Process; Property; Relative (related person); Research; Role; Route; Slice; Staining method; Stains; Synapses; Testing; Thalamic structure; Tracer; Virus; Weight; Whole-Cell Recordings; Work; base; behavior change; biocytin; improved; information processing; insight; nervous system disorder; novel; optical imaging; research study; response

DESCRIPTION (provided by applicant): The goals of this study will be to investigate the synaptic properties of auditory cortical synapses, in order to assess their putative roles in auditory information processing. The broad objectives of this research are to understand the functional contributions of these projections to auditory perception and behavior and how changes in these synaptic properties manifest with age and in disease processes. I have already identified several anatomical and physiological properties of glutamatergic synapses in the central auditory system, which forms the basis for the proposed investigation. This study will utilize an in vitro slice preparation in the mouse, which has been developed for the auditory cortical pathways. Whole-cell recordings in cortical neurons will be used to record responses to minimal electrical stimulation, photostimulation by uncaging of glutamate, and photostimulation of channelrhodopsin expressing fibers in order to characterize their topographic organization and physiological properties. In addition, a novel method of optical imaging of responses revealed by autofluorescence will be employed to help trace the output patterns of auditory cortical projections. Morphological correlates will be sought using intracellular injections of biocytin. The proposed experiments should lead to further insight on the information-bearing roles of the auditory cortical projections, as well as help to establish their relative weights in the organization of central processing in the auditory system.

描述（由申请人提供）：本研究的目标是研究听觉皮层突触的突触特性，以评估它们在听觉信息处理中的假定作用。这项研究的主要目标是了解这些投射对听觉感知和行为的功能贡献，以及这些突触特性的变化如何随着年龄和疾病过程而表现出来。我已经确定了中枢听觉系统中谷氨酸能突触的几个解剖学和生理学特性，这构成了拟议研究的基础。这项研究将利用小鼠的体外切片制剂，该制剂是为听觉皮层通路开发的。皮层神经元的全细胞记录将用于记录对最小电刺激、谷氨酸释放的光刺激以及视紫红质通道蛋白表达纤维的光刺激的反应，以表征其拓扑组织和生理特性。此外，将采用一种对自发荧光揭示的反应进行光学成像的新方法来帮助追踪听觉皮层投射的输出模式。将通过细胞内注射生物胞素来寻找形态学相关性。所提出的实验应该可以进一步了解听觉皮层投射的信息承载作用，并有助于确定它们在听觉系统中央处理组织中的相对权重。