Functional architecture of the auditory cortex

听觉皮层的功能结构

• 批准号: 8196927
• 负责人: CHARLES C LEE
• 金额: $ 14.8万
• 依托单位: LOUISIANA STATE UNIV A&M COL BATON ROUGE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-12-01 至 2013-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8196927
• 关键词: Age; Aging; Anatomy; Architecture; Area; Auditory; Auditory Perception; Auditory Physiology; Auditory area; Auditory system; Diagnostic; Disease; Electric Stimulation; Electron Microscopy; Feedback; Fiber; Foundations; Glutamates; Goals; In Vitro; Injection of therapeutic agent; Investigation; Knowledge; Label; Lead; Methods; Midbrain structure; Morphology; Mus; Neurons; Output; Pathway interactions; Patients; Pattern; Physiological; Physiology; Preparation; Process; Property; Relative (related person); Research; Role; Route; Slice; Staining method; Stains; Synapses; Testing; Thalamic structure; Tracer; Virus; Weight; Whole-Cell Recordings; Work; base; behavior change; biocytin; improved; information processing; insight; nervous system disorder; novel; optical imaging; optogenetics; public health relevance; research study; response

DESCRIPTION (provided by applicant): The goals of this study will be to investigate the synaptic properties of auditory cortical synapses, in order to assess their putative roles in auditory information processing. The broad objectives of this research are to understand the functional contributions of these projections to auditory perception and behavior and how changes in these synaptic properties manifest with age and in disease processes. I have already identified several anatomical and physiological properties of glutamatergic synapses in the central auditory system, which forms the basis for the proposed investigation. This study will utilize an in vitro slice preparation in the mouse, which has been developed for the auditory cortical pathways. Whole-cell recordings in cortical neurons will be used to record responses to minimal electrical stimulation, photostimulation by uncaging of glutamate, and photostimulation of channelrhodopsin expressing fibers in order to characterize their topographic organization and physiological properties. In addition, a novel method of optical imaging of responses revealed by autofluorescence will be employed to help trace the output patterns of auditory cortical projections. Morphological correlates will be sought using intracellular injections of biocytin. The proposed experiments should lead to further insight on the information-bearing roles of the auditory cortical projections, as well as help to establish their relative weights in the organization of central processing in the auditory system.

描述（由申请人提供）：本研究的目的是研究听觉皮层突触的突触特性，以评估其在听觉信息处理中的假定作用。这项研究的广泛目标是了解这些投射对听觉感知和行为的功能贡献，以及这些突触特性如何随年龄和疾病过程而变化。我已经确定了中枢听觉系统中突触的几个解剖学和生理学特性，这构成了拟议研究的基础。本研究将利用小鼠体外切片制备，其已开发用于听觉皮层通路。皮质神经元中的全细胞记录将用于记录对最小电刺激、通过释放谷氨酸盐的光刺激和通道视紫红质表达纤维的光刺激的响应，以表征其地形组织和生理特性。此外，一种新的方法，光学成像的反应揭示了自发荧光将有助于跟踪输出模式的听觉皮层的预测。形态学的相关性将寻求使用细胞内注射的生物胞素。拟议的实验应导致进一步了解听觉皮层预测的信息承载作用，以及帮助建立其相对权重的组织中的听觉系统的中央处理。