Atherosclerosis and Lipoprotein Analysis Core

动脉粥样硬化和脂蛋白分析核心

• 批准号: 8242737
• 负责人: Jonathan D Smith
• 金额: $ 26.11万
• 依托单位: CLEVELAND CLINIC LERNER COM-CWRU
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8242737
• 关键词: Adventitious Tissue; Aorta; Area; Atherosclerosis; Biological Assay; Blood Vessels; Cells; Collaborations; Core Facility; Data; In Situ; Individual; Inflammatory Response; Laboratories; Learning; Lesion; Lipids; Lipoproteins; Microscope; Mus; Perfusion; Plant Roots; Preparation; Protocols documentation; Saline; Slide; Staining method; Stains; Standardization; Surface; Thoracic aorta; Tissues; Transgenic Mice; abdominal aorta; aortic arch; investigator training; oil red O

leader's approved protocol to: 1) anesthetize the mice, 2) perform saline perfusion, which euthanizes the mouse by exsanguination, 3) dissect the mouse to display the mouse aorta in situ, 4) remove the aorta and trim off adventitial tissue, 5) cut the aorta longitudinally and fix it flattened between two microscope slides, 6) stain the aorta with oil red O, 7) mount the aorta on a microscope slide, and 8) quantify the % surface lesion area in the entire aorta, as well as in the regions of the aortic arch, and thoracic and abdominal aortas. Utilization of the Core Facility

领导批准的方案：1)麻醉小鼠，2) 进行生理盐水灌流，放血安乐死小鼠，3)解剖小鼠显示 小鼠在体主动脉，4)切除大鼠主动脉，切除外膜组织，5)纵向切断大鼠主动脉并固定。 在两张显微镜载玻片之间压平，6)用油红染色主动脉，7)在显微镜上安装主动脉 幻灯片，以及8)量化整个主动脉以及主动脉弓区域的表面损伤面积百分比， 胸主动脉和腹主动脉。 核心设施的利用情况