Atherosclerosis and Lipoprotein Analysis Core
Atherosclerosis and Lipoprotein Analysis Core
批准号:
8242737
负责人:
Jonathan D Smith
金额:
$26.11万
依托单位国家:
美国
项目类别:
财政年份:
2011
资助国家:
美国
项目状态:
已结题
起止时间:
2011-04-01 至 2014-03-31
关键词:
Adventitious TissueAortaAreaAtherosclerosisBiological AssayBlood VesselsCellsCollaborationsCore FacilityDataIn SituIndividualInflammatory ResponseLaboratoriesLearningLesionLipidsLipoproteinsMicroscopeMusPerfusionPlant RootsPreparationProtocols documentationSalineSlideStaining methodStainsStandardizationSurfaceThoracic aortaTissuesTransgenic Miceabdominal aortaaortic archinvestigator trainingoil red O
中文摘要
领导人批准的方案:1)麻醉小鼠,2)
进行盐水灌注,其通过放血使小鼠安乐死,3)解剖小鼠以显示
4)切除主动脉并修剪外膜组织,5)纵切主动脉并固定
在两个显微镜载玻片之间压平,6)用油红O染色主动脉,7)将主动脉安装在显微镜上
幻灯片,以及8)量化整个主动脉中以及主动脉弓区域中的表面损伤面积%,
以及胸腹部的动脉瘤
核心设施的利用
英文摘要
leader's approved protocol to: 1) anesthetize the mice, 2)
perform saline perfusion, which euthanizes the mouse by exsanguination, 3) dissect the mouse to display the
mouse aorta in situ, 4) remove the aorta and trim off adventitial tissue, 5) cut the aorta longitudinally and fix it
flattened between two microscope slides, 6) stain the aorta with oil red O, 7) mount the aorta on a microscope
slide, and 8) quantify the % surface lesion area in the entire aorta, as well as in the regions of the aortic arch,
and thoracic and abdominal aortas.
Utilization of the Core Facility
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
Project 1 - Genes to Function: Causal Genes and their Roles in Cardiomyocyte and Atrial Physiology
-
批准号:10646358
-
项目类别:
-
资助金额:$50.72万
-
财政年份:2022
-
负责人:Jonathan D Smith
-
依托单位:
Project 1 - Genes to Function: Causal Genes and their Roles in Cardiomyocyte and Atrial Physiology
-
批准号:10410648
-
项目类别:
-
资助金额:$50.72万
-
财政年份:2022
-
负责人:Jonathan D Smith
-
依托单位:
Genetic modifiers of atherosclerosis and macrophage phenotypes
-
批准号:10306932
-
项目类别:
-
资助金额:$63.26万
-
财政年份:2021
-
负责人:Jonathan D Smith
-
依托单位:
Molecular Medicine Training Program at Cleveland Clinic/Case Western Reserve University
-
批准号:10426323
-
项目类别:
-
资助金额:$31.22万
-
财政年份:2021
-
负责人:Jonathan D Smith
-
依托单位:
Molecular Medicine Training Program at Cleveland Clinic/Case Western Reserve University
-
批准号:10268038
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项目类别:
-
资助金额:$29.26万
-
财政年份:2021
-
负责人:Jonathan D Smith
-
依托单位:
Molecular Medicine Training Program at Cleveland Clinic/Case Western Reserve University
-
批准号:10620326
-
项目类别:
-
资助金额:$31.83万
-
财政年份:2021
-
负责人:Jonathan D Smith
-
依托单位:
Genetic modifiers of atherosclerosis and macrophage phenotypes
-
批准号:10626053
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项目类别:
-
资助金额:$61.88万
-
财政年份:2021
-
负责人:Jonathan D Smith
-
依托单位:
Oxidant resistant apoA1 in reverse cholesterol transport, inflammation and atherosclerosis
-
批准号:9451333
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项目类别:
-
资助金额:$39.63万
-
财政年份:2016
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负责人:Jonathan D Smith
-
依托单位:
Mechanism of ApoA1 Lipidation by ABCA1 in HDL biogenesis
-
批准号:9102483
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项目类别:
-
资助金额:$40.76万
-
财政年份:2016
-
负责人:Jonathan D Smith
-
依托单位:
Oxidant resistant apoA1 in reverse cholesterol transport, inflammation and atherosclerosis
-
批准号:9173990
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项目类别:
-
资助金额:$39.63万
-
财政年份:2016
-
负责人:Jonathan D Smith
-
依托单位:
ApoA1 lipidation by ABCA1 in HDL biogenesis
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批准号:10206232
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项目类别:
-
资助金额:$47.95万
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财政年份:2016
-
负责人:Jonathan D Smith
-
依托单位:
Oxidant resistant apoA1 in reverse cholesterol transport, inflammation and atherosclerosis
-
批准号:9276118
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项目类别:
-
资助金额:$39.63万
-
财政年份:2016
-
负责人:Jonathan D Smith
-
依托单位:
ApoA1 lipidation by ABCA1 in HDL biogenesis
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批准号:10642780
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项目类别:
-
资助金额:$47.95万
-
财政年份:2016
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负责人:Jonathan D Smith
-
依托单位:
Characterization of Atherosclerosis Modifier Genes
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批准号:8131145
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项目类别:
-
资助金额:$46.65万
-
财政年份:2010
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负责人:Jonathan D Smith
-
依托单位:
Characterization of Atherosclerosis Modifier Genes
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批准号:8280217
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项目类别:
-
资助金额:$46.66万
-
财政年份:2010
-
负责人:Jonathan D Smith
-
依托单位:
Characterization of Atherosclerosis Modifier Genes
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批准号:8490709
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项目类别:
-
资助金额:$44.42万
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财政年份:2010
-
负责人:Jonathan D Smith
-
依托单位:
Characterization of Atherosclerosis Modifier Genes
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批准号:7983316
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项目类别:
-
资助金额:$46.57万
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财政年份:2010
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负责人:Jonathan D Smith
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依托单位:
ABCA1, ApoAI and Reverse Cholesterol Transport
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批准号:8015694
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项目类别:
-
资助金额:$51.26万
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财政年份:2010
-
负责人:Jonathan D Smith
-
依托单位:
Atherosclerosis and Lipoprotein Analysis Core
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批准号:7659846
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项目类别:
-
资助金额:$10.82万
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财政年份:2009
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负责人:Jonathan D Smith
-
依托单位:
Genetics of Atherosclerosis in a Murine Model
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批准号:7786022
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项目类别:
-
资助金额:$48.83万
-
财政年份:2009
-
负责人:Jonathan D Smith
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依托单位:
海外基金