Imaging Core

自2004年以来，Imaging Core一直支持NYMC的氧化还原信号和血管生物学项目，并增加了其产量和能力，以满足不断增长的研究需求。Imaging Core的潜力是基于其领导者、顾问和其他研究人员的科学专业知识，他们在细胞和分子成像领域有着良好的记录。将成像技术整合到生理学研究中已经允许产生重要的新概念的实验范例，并且提供了对用于治疗的疾病模型中的病理生理学机制的重要见解。 部门在上一个资助期间，我们已经发表或即将发表几篇同行评审论文，使用成像核心设施为3个项目提供的技术和方法（见G节）。成像核心设施设备齐全，目前有一名技术员。学院完全致力于在必要时扩大核心基金的范围。Imaging Core可以使用高度复杂的仪器，这些仪器共同构成了一套独特的成像设备，其分辨率适合于分析活体血管中的ROS信号。所有服务将可用于小鼠、大鼠、牛、犬和人体组织以及基于细胞的研究。具体而言，成像核心将： 1.提供各种服务，以协助测量心血管组织中的ROS（包括O{2}[-]和H{2}O{2}）产生， 2.提供专业知识，设施和培训，为个人进行免疫细胞化学，免疫组织化学和血管组织的组织学（包括显微镜的使用和计算机辅助图像分析，并建立协议）。核心还将负责数据共享、数据储存和质量控制服务。它还为研究员和学生提供显微技术培训。 3.为使用LCM分析特定血管细胞类型中的基因表达提供支持。 成像核心将促进所有三个项目成像和监测心血管组织中的ROS产生，检测和定位血管细胞中的蛋白质表达和3-硝基酪氨酸含量，并显微解剖内皮细胞和平滑肌细胞以及心肌细胞进行差异基因表达分析。

The Imaging Core has supported redox signaling and vascular biology projects at NYMC since 2004 and has increased its output and capabilities to meet growing research demands. The potential of the Imaging Core is based on the scientific expertise of its leader, consultants and of the other investigators, who have strong track records in the area of cellular and molecular imaging. The integration of imaging techniques into physiological studies has allowed for experimental paradigms that have generated important new concepts as well as providing significant insights into the pathophysiological mechanisms in disease models used in the Department. Over the previous funding period, we have published or are about to publish several peer-reviewed papers, using the technologies and methods provided by the Imaging Core facility for the 3 projects (see section G). The Imaging Core Facility is fully equipped and currently staffed by one technician. The College is fully committed to expanding the scope of the Core Facility as need arises. The Imaging Core has access to highly sophisticated instruments, which taken together, constitute a unique set of imaging apparatus in terms of achieving a resolution power suitable for the analysis of ROS signaling in living blood vessels. All services will be available for mouse, rat, bovine, dog and human tissues as well as for cell-based studies. Specifically the Imaging Core will: 1. offer a variety of services to assist with measurements of ROS (including O{2}[-] and H{2}O{2}) production in cardiovascular tissues, and 2. provide expertise, facilities and training for individuals in performing immunocytochemistry, immunohistochemistry, and histology of vascular tissues (including microscope usage and computer-assisted image analyses, and establishing protocols). The Core will also be responsible for data sharing, data storage and quality control services. It also provides training for fellows and students in microscopy techniques. 3. provide support for analysis of gene expression in specific vascular cell types using LCM. The Imaging Core will facilitate all three of the Projects to image and monitor ROS production in cardiovascular tissues, to detect and localize protein expression and 3-nitrotyrosine content in vascular cells and microdissect endothelial and smooth muscle cells and cardiomyocytes for differential gene expression analysis.