Role of TNFa in Osteoclast-Mediated Bone Loss

TNFa 在破骨细胞介导的骨丢失中的作用

• 批准号: 7929036
• 负责人: LIANPING XING
• 金额: $ 21.36万
• 依托单位: UNIVERSITY OF ROCHESTER
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-18 至 2011-09-17
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7929036
• 关键词: Affect; Antibodies; Arthritis; Autoimmunity; Biology; Blood; Bone Resorption; Cells; Data; Development; Effector Cell; Etiology; Family member; Figs - dietary; Funding; Growth Factor; Histology; Hyperplasia; Immunohistochemistry; In Vitro; Inflammation; Inflammatory; Interruption; Intervention; Joints; Knock-out; Lead; Link; Lymphangiogenesis; Lymphatic; Lymphatic Endothelial Cells; Lymphatic System; Lymphatic vessel; Magnetic Resonance Imaging; Mediating; Microarray Analysis; Molecular; Mus; Osteitis; Osteoclasts; Pathogenesis; Pathologic Processes; Pathway interactions; Patients; Pattern; Play; Production; Proto-Oncogene Proteins c-akt; Psoriatic Arthritis; Recombinant adeno-associated virus (rAAV); Rheumatoid Arthritis; Role; Sampling; Series; Severities; Signal Pathway; Signal Transduction; Signaling Protein; Syndrome; TNF gene; TNFSF11 gene; Testing; Therapeutic Agents; Vascular Endothelial Growth Factor C; Vascular Endothelial Growth Factor Receptor; Vascular Endothelial Growth Factor Receptor-2; Vascular Endothelial Growth Factors; adeno-associated viral vector; arthropathies; autocrine; base; bone; bone loss; cytokine; in vivo; joint destruction; lymph nodes; mouse model; novel; osteoclastogenesis; paracrine; prevent; public health relevance; receptor; research study; response; tumor necrosis factor precursor; vasculogenesis

DESCRIPTION (provided by applicant): Bone destruction in inflammatory erosive arthritis, such as rheumatoid arthritis (RA), is associated with increased production of pro-inflammatory cytokines, which promote focal bone resorption by increasing the differentiation of osteoclast precursors (OCP) to mature osteoclasts in inflamed joints. Recent progress in Osteoimmunology indicates that osteoclasts are not only bone resorbing cells; they also produce factors that contribute to inflammation and autoimmunity by autocrine and paracrine mechanisms. However, osteoclast-produced factors that could affect the development and progression of RA directly have not been well studied. Using microarray analysis, we identified that VEGF-C expression is significantly increased in OCPs from TNF- Tg arthritic mice. VEGF-C is a lymphogenic growth factor essential for lymphoangiogenesis. But its role in osteoclast function has not been investigated and little is known about the role of the lymphatic system in the pathogenesis of joint destruction in RA. To explore the involvement of VEGF-C in this setting, we carried out a series preliminary studies and demonstrated that 1) RANKL and TNF induce VEGF-C expression in OCPs and OCs; 2) VEGF-C stimulates osteoclastic bone resorption; 3) RA joints have remarkably increased lymphatic vessel formation; and 4) inhibition of VEGF-C signaling reduces the severity of joint inflammation in TNF-Tg mice. Based on these findings, we hypothesize that in inflammatory joints OCPs and OCs are activated by RANKL and TNF to produce VEGF-C, which mediates joint inflammation and bone erosion simultaneously by stimulating vasculogenesis and osteoclastogenesis via autocrine and paracrine mechanisms. These hypotheses will be tested in 3 specific aims. In Aim 1, we will investigate the mechanisms by which RANKL and TNF stimulate OCPs and osteoclasts to produce VEGF-C. In Aim 2, we will examine the effects of VEGF-C on osteoclast function and the downstream signaling pathways involved. In Aim 3, we will determine the functional importance of VEGF-C signaling in the pathogenesis of RA using a VEGFR3 blockade approach. These studies will provide new information on how OCPs stimulate pannus formation via lymphangiogenesis. Ultimately they should lead to the development of novel specific therapeutic agents to prevent and treat patients with inflammatory erosive arthritis. PUBLIC HEALTH RELEVANCE. The proposed study is aimed to investigate the role of bone resorbing osteoclasts in the development and progression of rheumatoid arthritis by affecting osteoclast function and lymphangiogenesis in mouse models of arthritis. The results will enhance our understanding of osteoclast and lymphatic biology and provide new direction for developing a novel therapy to treat arthritis.

描述（由申请人提供）：炎症性糜糜性关节炎（如类风湿关节炎（RA））的骨破坏与促炎细胞因子的产生增加有关，促炎细胞因子通过增加炎症关节中破骨细胞前体（OCP）向成熟破骨细胞的分化来促进局灶性骨吸收。近年来骨免疫学的研究进展表明，破骨细胞不仅仅是骨吸收细胞；它们还通过自分泌和旁分泌机制产生促进炎症和自身免疫的因子。然而，破骨细胞产生的因子可能直接影响RA的发生和进展尚未得到很好的研究。通过微阵列分析，我们发现TNF- Tg关节炎小鼠ocp中VEGF-C的表达显著增加。VEGF-C是淋巴血管生成所必需的淋巴生长因子。但其在破骨细胞功能中的作用尚未被研究，对淋巴系统在RA关节破坏发病机制中的作用知之甚少。为了探讨VEGF-C在这种情况下的参与，我们进行了一系列初步研究，并证明：1)RANKL和TNF诱导ocp和OCs中VEGF-C的表达；2) VEGF-C刺激破骨细胞骨吸收；3) RA关节淋巴血管形成明显增加；4)抑制VEGF-C信号可减轻TNF-Tg小鼠关节炎症的严重程度。基于这些发现，我们假设炎症关节中ocp和OCs被RANKL和TNF激活产生VEGF-C， VEGF-C通过自分泌和旁分泌机制刺激血管生成和破骨细胞生成，同时介导关节炎症和骨侵蚀。这些假设将在三个具体目标中进行检验。在Aim 1中，我们将研究RANKL和TNF刺激ocp和破骨细胞产生VEGF-C的机制。在Aim 2中，我们将研究VEGF-C对破骨细胞功能的影响及其下游信号通路。在Aim 3中，我们将使用VEGFR3阻断方法确定VEGF-C信号在RA发病机制中的功能重要性。这些研究将为ocp如何通过淋巴管生成刺激肠膜形成提供新的信息。最终，它们将导致新的特异性治疗剂的发展，以预防和治疗炎症性糜烂性关节炎患者。公共卫生相关性。本研究旨在通过影响关节炎小鼠模型的破骨细胞功能和淋巴管生成，探讨骨吸收破骨细胞在类风湿关节炎发生发展中的作用。该结果将增强我们对破骨细胞和淋巴生物学的认识，并为开发治疗关节炎的新疗法提供新的方向。