Musculoskeletal Structure and Strength

在评估动物模型时，组织结构和强度是最相关的属性 肌肉骨骼损伤、疾病和修复。治疗效果是否由遗传因素造成 操作、代谢挑战、模拟损伤、手术修复或其他干预，净效果必须 根据它们是否导致了更多或更少的组织，该组织是否正常 形态，以及组织或骨骼结构是否增加或降低了机械性能。 我们有必要的设备和证明的用户专业知识来评估结构和 来自动物模型的一系列肌肉骨骼组织和结构的机械特性。 在研究基地的调查人员和研究人员之间进行了卓有成效的合作 这个核心，但这些在数量上受到限制，因为缺乏支持收购和 专家用户的数据分析和非专家用户的培训。这种肌肉骨骼的目的是 结构和实力核心(核心B)是提供一种机制，以增加对现有资源的访问 基于X射线的密度测量和成像以及机械测试，从而实现新的相互作用和 加强华盛顿大学肌肉骨骼研究人员之间的现有互动。我们会 维护协议和设备以促进质量控制，提供技术支持和培训，以及 对动物模型的肌肉骨骼结构和组织执行以下服务(小鼠到 犬)由核心中心研究基地的研究人员产生。目的1：基于X射线的成像； 可用的方式包括X光照相、双能X射线吸收法(DXA)、外周定量 计算机断层扫描(PQCT)和微型计算机断层扫描(MicroCT)。其中每一项都可用于EX 活体或活体成像。目标2：机械测试；可用的形式包括全骨弯曲和 压缩、骨小梁压痕、脱矿化骨、肌腱和肌腱-骨植入的拉伸测试 现场拉伸试验；肌力测量。通过提供这些核心服务，我们将增加研究 提高我校现有肌肉骨骼研究人员的工作效率，促进非肌肉骨骼研究 希望为肌肉骨骼生物学和医学研究带来新视角的研究人员。

Tissue structure and strength are the most relevant properties when assessing animal models related to musculoskeletal injury, disease and repair. Whether the treatment effects are created by genetic manipulation, metabolic challenge, simulated injury, surgical repair or other intervention, the net effects must be judged based on whether they have resulted in more or less tissue, whether that tissue has normal morphology, and whether the tissue or skeletal structure has increased or decreased mechanical properties. We have the necessary equipment and the demonstrated user expertise to assess the structural and mechanical properties of a range of musculoskeletal tissue and structures derived from animal models. There have been productive collaborations between investigators in the Research Base and the personnel of this Core, but these have been limited in number by the lack of a mechanism to support the acquisition and analysis of data by expert users and the training of non-expert users. The objective of this Musculoskeletal Structure and Strength Core (Core B) is to provide a mechanism to increase access to existing resources for x-ray based densitometry and imaging, and mechanical testing, and to thereby enable new interactions and enhance existing interactions between musculoskeletal researchers at Washington University. We will maintain protocols and equipment to promote quality control, provide technical support and training, and perform the following services on musculoskeletal structures and tissues from animal models (mouse to canine) generated by investigators in the Core Center Research Base. Aim 1: X-ray based imaging; available modalities include radiography, dual-energy x-ray absorptiometry (DXA), peripheral quantitative computed tomography (pQCT) and micro-computed tomography (microCT). Each of these is available for ex vivo or in vivo imaging. Aim 2: Mechanical testing; available modalities include whole-bone bending and compression, trabecular indentation, tensile testing of demineralized bone, tendon and tendon-bone insertion site tensile testing; muscle force measurement. By offering these Core services we will increase research productivity of established musculoskeletal investigators on our campus and facilitate non-musculoskeletal investigators wishing to bring new perspectives to studies in musculoskeletal biology and medicine.