Resource Based Center for Musculoskeletal Biology and Medicine (Overall Application)

摘要/摘要-总体 在美国，每两个成年人中就有一个患有普遍的肌肉骨骼疾病，相关费用为900美元。 每年十亿。为了解决这一危机，必须有效利用资源，并持续致力于基本的 翻译研究是必不可少的。我们建议设立华盛顿大学资源中心， 肌肉骨骼生物学和医学，致力于两个长期目标-推进研究， 肌肉骨骼疾病的生物学基础，并开发和测试基于生物学的干预措施 战略布局我们代表了一个由81名研究人员组成的动态研究社区，隶属于17个学术机构， 部门自2009年以来，该中心已贡献了343篇出版物，被引用6687次。 中心调查人员的年度外部资金为6620万美元，其中包括来自NIAMS的810万美元。我们 工作重点是最普遍和昂贵的肌肉骨骼疾病-关节炎，背痛，骨和 关节创伤骨质疏松和转移性癌症值得注意的是，我们的大部分研究工作都在 基础和临床前研究。因此，我们的总体目标是支持研究社区， 肌肉骨骼生物学和医学动物模型的开发、实施和评估。 我们将通过四个综合目标来实现这一目标。目标1。通过行政核心（A）， 提供中心领导，以管理核心并参与外展。指导一个强化计划 这将促进下一代肌肉骨骼研究人员的发展，通过试点和 可持续发展计划，研讨会，座谈会和其他活动。目标二。通过肌肉骨骼结构 和实力核心（B），支持访问、培训和具有成本效益的利用：i）基于X射线的成像 方法来量化形态，和ii）生物力学方法来量化的机械性能， 肌肉骨骼组织（骨、肌腱、肌肉、软骨、椎间盘）。目标3：通过肌肉骨骼 组织学和形态测定核心（C），进行组织处理、包埋、切片和染色， 方案维护和用户培训适用于所需的组织/技术范围 肌肉骨骼研究支持常规和高级显微镜检查的访问和培训， 组织形态计量学目标4。在关节损伤和疾病核心（D）的动物模型中，提供培训和 用于骨关节炎和类风湿性关节炎的可再现小鼠模型的实施。体内测量 生物力学和行为来评估关节疼痛和功能。建立一个组织和血清库 来自关节炎小鼠模型的样品。总括而言，我们建议的四个核心， 为我们的研究社区开发，实施和评估肌肉骨骼的动物模型 生物学和医学。每个核心的共同主题是支持培训和充实活动， 培养下一代肌肉骨骼研究人员。

Summary/Abstract - Overall One in two adults in the United States has a prevalent musculoskeletal condition, with related costs of $900 billion annually. To address this crisis, efficient utilization of resources and sustained commitment to basic and translational research are essential. We propose the Washington University Resource-Based Center for Musculoskeletal Biology and Medicine, dedicated to two long-term goals – to advance research on the biological basis of musculoskeletal disease, and to develop and test biologically based intervention strategies. We represent a dynamic Research Community of 81 investigators, affiliated with 17 academic departments. Since 2009, the Center has contributed to 343 publications that have been cited 6687 times. The annual external funding of Center investigators is $66.2 million, including $8.1 million from NIAMS. Our work focuses on the most prevalent and costly musculoskeletal disorders – arthritis, back pain, bone and joint trauma, osteoporosis, and metastatic cancer. Notably, the majority of our research endeavors are in basic and pre-clinical studies. Therefore, our overall objective is to support the Research Community in the development, implementation and evaluation of animal models for musculoskeletal biology and medicine. We will address this objective through four integrated Aims. Aim 1. Through an Administrative Core (A), provide Center leadership to manage the Cores and engage in outreach. Direct an Enrichment Program that will foster the development of the next generation of musculoskeletal investigators through a Pilot & Feasibilty program, seminars, symposia and other activities. Aim 2. Through the Musculoskeletal Structure and Strength Core (B), support access, training and cost-effective utilization for: i) x-ray based imaging methods to quantify morphology, and ii) biomechanical methods to quantify mechanical properties of musculoskeletal tissues (bone, tendon, muscle, cartilage, disc). Aim 3. Through the Musculoskeletal Histology and Morphometry Core (C), perform tissue processing, embedding, sectioning and staining, protocol maintenance and user training appropriate for the range of tissues/techniques required in musculoskeletal research. Support access and training for routine and advanced microscopy and histomorphometry. Aim 4. In the Animal Models of Joint Injury and Disease Core (D), provide training and implementation for reproducible mouse models of osteoarthritis and rheumatoid arthritis. Measure in vivo biomechanics and behavior to assess joint pain and function. Create a repository of tissue and serum samples from arthritic mouse models. In summary, the four Cores we propose will provide essential support for our Research Community to develop, implement and evaluate animal models for musculoskeletal biology and medicine. A common theme for each Core is to support training and enrichment activities to foster development of the next generation of musculoskeletal investigators.