Musculoskeletal Structure and Strength Core

肌肉骨骼结构和核心力量

• 批准号: 10602565
• 负责人: MATTHEW J SILVA
• 金额: $ 14.35万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-04-15 至 2024-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10602565
• 关键词: Address; Adherence; Animal Model; Autopsy; Biology; Biomechanics; Cartilage; Communities; Data; Development; Dual-Energy X-Ray Absorptiometry; Ensure; Equipment; Evaluation; Exhibits; Fees; Fostering; Fracture; Funding; Future; Goals; Human Resources; Image; Image Enhancement; Individual; Investments; Manuscripts; Measures; Mechanics; Medicine; Methods; Microscope; Morphology; Muscle; Muscle Tension; Musculoskeletal; Musculoskeletal structure; Outcome; Productivity; Property; Protocols documentation; Publications; Reporting; Research; Research Personnel; Resolution; Resources; Roentgen Rays; Schedule; Services; Structure; Techniques; Tendon structure; Testing; Tissues; Training; Training Programs; Universities; Washington; animal tissue; bone; cellular imaging; contrast enhanced; cost effective; functional outcomes; imaging approach; in vivo; instrument; long bone; mechanical properties; member; microCT; musculoskeletal imaging; notch protein; novel strategies; online resource; programs; radiological imaging; response; skeletal disorder; spine bone structure; submicron

Summary – Resource Core B Measures of structure and strength are essential when assessing functional outcomes in animal models related to musculoskeletal biology and medicine. Evaluating these outcomes requires specialized equipment and expertise, which is impractical and inefficient to recreate in multiple labs. In response to this need, we established the Musculoskeletal Structure and Strength Core. This multifaceted resource supports our Research Community in understanding and implementing techniques to evaluate structure and mechanical properties of musculoskeletal tissues (bone, cartilage, disc, muscle, tendon) derived from animal models. We have had a strong impact. Since 2009, we have provided billable services to 94 unique investigators, who have reported structure or strength data in 176 manuscripts which have been cited 3266 times. The need for these services remains high, with 57 current and projected future users. In order to sustain and extend this resource going forward, we propose two Specific Aims. Aim 1 – Imaging: Maintain protocols and equipment, provide training and technical support, and perform imaging of musculoskeletal structures and tissues from animal models generated by Research Community investigators. Routine techniques include plane radiography, dual-energy X-ray absorptiometry (DXA), and micro-computed tomography (microCT), each available post mortem or in vivo. We have recently developed methods for contrast-enhanced imaging; we will make these available to our users and will continue to develop new approaches for imaging non-mineralized tissues (cartilage, disc, muscle, tendon). We will present regular seminars on imaging and strengthen our partnership with the Center for Cellular Imaging. Aim 2 – Mechanical Testing: Perform mechanical testing to assess the functional properties of musculoskeletal structures and tissues from animal models generated by Research Community investigators. Standard testing methods include long-bone bending, vertebral compression, tendon and tendon-to-bone tensile testing, and bone microindentation. Newly established methods include passive and active muscle tension testing and notched bending for fracture toughness. We will present seminars and provide training and enrichment to enhance understanding of biomechanics among Center investigators. In summary, Core B will provide critical support to members of our large Research Community to enable cost-effective utilization of methods to quantify morphology and mechanical properties from musculoskeletal tissues (bone, tendon, muscle, cartilage, disc). Through our efforts we will enhance research productivity and rigor, and provide access to state-of-the-art and emerging techniques.

摘要-资源核心B 在评估动物模型的功能结果时，结构和强度的测量是必不可少的 与肌肉骨骼生物学和医学相关。评估这些结果需要专业的 设备和专业知识，这是不切实际的，效率低下，在多个实验室重新创建。针对这一 根据需要，我们建立了肌肉骨骼结构和力量核心。这种多方面的资源支持 我们的研究社区在理解和实施技术，以评估结构和 肌肉骨骼组织（骨、软骨、椎间盘、肌肉、肌腱）的机械性能， 动物模型我们产生了强烈的影响。自2009年以来，我们已向94个独特的 研究者在176篇论文中报告了结构或强度数据，被引用3266次 次对这些服务的需求仍然很大，目前和预计未来有57个用户。为了 为了维持和扩大这一资源，我们提出了两个具体目标。目标1-成像：维持 协议和设备，提供培训和技术支持，并进行肌肉骨骼成像 结构和组织的动物模型产生的研究社区的调查。常规 技术包括平面X线摄影、双能X线吸收测定法（DXA）和微计算机 断层扫描（microCT），每一个死后或体内可用。我们最近开发了一种方法， 对比度增强成像;我们将向我们的用户提供这些，并将继续开发新的 非矿化组织（软骨、椎间盘、肌肉、肌腱）成像方法。我们将定期 我们还将举办成像研讨会，并加强我们与细胞成像中心的合作关系。目标2- 力学测试：进行力学测试，以评估肌肉骨骼的功能特性 结构和组织的动物模型产生的研究社区的调查。标准 测试方法包括长骨弯曲、椎骨压缩、肌腱和肌腱-骨拉伸 测试和骨微压痕。新建立的方法包括被动和主动肌肉紧张 断裂韧性的测试和缺口弯曲。我们将举办研讨会，提供培训， 丰富内容，以增强中心研究者对生物力学的理解。总之，核心B 将为我们的大型研究社区成员提供关键支持，以实现成本效益的利用 对肌肉骨骼组织（骨，腱， 肌肉、软骨、椎间盘）。通过我们的努力，我们将提高研究的生产力和严谨性，并提供 获得最先进和新兴技术。