Flowcytometry Core

流式细胞术是用于分析和分离细胞因子的最广泛使用的和不可缺少的技术之一。 特定的细胞群体。CFAR流式细胞术核心于2003年成立，最初的种子资金 从CFAR拨款中拨款，为CFAR研究人员提供更好的先进流式细胞术 设备和技术。CFAR核心在爱因斯坦的流式细胞术资源中是独一无二的， 提供生物危害防护，以便对需要生物安全等级2的样本进行分析和分类 或更高的预防措施，因此是涉及艾滋病毒和各种艾滋病相关工作的主要资源 机会致病菌CFAR流式细胞术核心为CFAR研究者提供了随时访问 各种先进的分析流式细胞术和细胞分选仪器，以及广泛的技术支持， 专业知识和援助，以执行既定的流式细胞术技术和实施 这一领域的新技术。自成立以来的四年中，核心已经大幅增长， 在空间、设备和人员方面。这反映了这一核心设施所发挥的重要作用 在CFAR相关的研究项目，也有相当大的机构支持，强调了价值 增加了核心的功能。核心小组的活动按以下四个具体目标组织： 目的： 目标1.为了维持流式细胞术研究所需的必要研究基础设施， 适当的生物安全防护措施。 目标2.通过定期更新现有流式细胞仪资源来保持最先进的能力， 及时引进新设备、新技术。 目标3。提供方便和具有成本效益的设备和技术专长， 流式细胞术的性能和解释。 目标4。提供教育和培训，以协助CFAR调查人员实施新的 建立流式细胞术技术。 相关性：流式细胞术使研究人员能够研究细胞或微生物的许多特性， 快速收集对了解动物模型和人类疾病过程至关重要的信息 科目CFAR流式细胞仪核心支持该类型所需的仪器和人员 的基本研究，并组织这些资源在一个集中的设施，提供访问的大 许多调查人员从事与艾滋病毒感染和艾滋病有关的广泛问题的研究。

Flow cytometry is one of the most widely used and indispensable techniques for the analysis and isolation of specific cell populations. The CFAR Flow Cytometry Core was established in 2003 with initial seed funds from the CFAR grant to provide CFAR investigators with improved access to advanced flow cytometry equipment and techniques. The CFAR Core is unique among the flow cytometry resources at Einstein in providing biohazard containment for carrying out analysis and sorting of samples requiring Biosafety Level-2 or higher precautions, and is thus a major resource for work involving HIV and a variety of AIDS-related opportunistic pathogens. The CFAR Flow Cytometry Core provides CFAR investigators with ready access to a variety of advanced instruments for analytical flow cytometry and cell sorting, and extensive technical expertise and assistance for performance of established flow cytometry techniques and implementation of new technologies in this area. In the four years since its inception, the Core has grown substantially with regard to space, equipment and personnel. This reflects the important role that this core facility has played in CFAR related research projects, and also the considerable institutional support that underscores the value added features of the Core. The activities of the Core are organized according to the following four specific aims: Aim 1. To maintain the necessary research infrastructure needed for flow cytometry studies, with appropriate biosafety containment safeguards. Aim 2. To maintain cutting edge capabilities by regularly updating existing flow cytometry resources and introducing new equipment and techniques in a timely manner. Aim 3. To provide convenient and cost-effective access to equipment and technical expertise for the performance and interpretation of flow cytometry. Aim 4. To provide education and training to assist CFAR investigators in the implementation of new and established flow cytometry techniques. RELEVANCE: Flow cytometry allows researchers to study many properties of cells or micro-organisms and rapidly gather information that is crucial to understanding disease processes in animal models and human subjects. The CFAR Flow Cytometry Core supports instruments and personnel that are required for this type of essential research, and organizes these resources in a centralized facilty that provides access for a large number of investigators working on a wide range of problems relevant to HIV infection and AIDS.