COBRE: NDSU: PROJECT 3: MMP-9 IN APOPTOSIS OF PROSTATE CANCEL CELLS

COBRE：NDSU：项目 3：MMP-9 在前列腺癌细胞凋亡中的作用

• 批准号: 8360596
• 负责人: BIN GUO
• 金额: $ 7.05万
• 依托单位: NORTH DAKOTA STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2012-09-23
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8360596
• 关键词: Apoptosis; Apoptotic; Basement membrane; Cell Survival; Cells; Centers of Research Excellence; Cleaved cell; Clinical Trials; Collagen; Disease; Down-Regulation; Epigenetic Process; Funding; Future; Gelatinase B; Grant; Inhibition of Matrix Metalloproteinases Pathway; Malignant - descriptor; Malignant neoplasm of prostate; Mammals; Matrix Metalloproteinase Inhibitor; Matrix Metalloproteinases; Mediating; National Center for Research Resources; Neoplasm Metastasis; Patients; Peptide Hydrolases; Principal Investigator; Prostate; Protein Isoforms; Regulation; Research; Research Infrastructure; Resources; Role; Signal Pathway; Signal Transduction; Signaling Molecule; Source; Staging; Treatment Efficacy; United States National Institutes of Health; cancer cell; cancer therapy; chemotherapy; cost; cytokine; inhibitor/antagonist; neoplastic cell; receptor; response; tumor progression

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Matrix metalloproteinases (MMPs) are promising targets for cancer therapy. However, recent clinical trials of MMP inhibitors have been disappointing. Certain MMPs may regulate apoptosis signaling pathways and sensitize tumor cells to apoptotic signals. Inhibition of these MMPs will promote tumor cell survival and this effect may offset the beneficial activity in inhibition of metastasis. Matrix metalloproteinase-9 (MMP-9) expression is significantly increased in malignant prostate cancers. In addition to its ability to cleave collagens and basement membrane components, MMP-9 also cleaves and activates the multifunctional cytokine TGF-¿. TGF-¿ (having three isoforms in mammals: TGF-¿1, ¿2, ¿3) is an important regulator of normal and malignant prostate. While TGF-¿1 induces apoptosis in certain prostate cancer cells, TGF-¿2 blocks apoptosis. By activating all three isoforms of TGF-¿, MMP-9 may have different net effect on prostate cancer apoptosis depending on the status of expression of TGF-¿ isoforms, their receptors, and the downstream signaling molecules. There is a critical need to determine the effects of MMP-9 on apoptosis in prostate cancer. Presumable, inhibition of MMP-9 will be beneficial in patients where MMP-9-mediated TGF-¿ activation has an anti-apoptotic effect in cancer cells. Selection of appropriate patients according to the status of TGF-¿ signaling machinery may be critical for future clinical trials to evaluate the therapeutic efficacy of MMP-9 inhibitors. The objective of this research is to define the role of MMP-9 in prostate cancer apoptosis and how the effects of MMP-9 on apoptosis change along with prostate cancer progression. At the completion of this project, we expect to clarify the role of MMP-9 in apoptosis regulation in prostate cancer and how this role may change in relation with the changes of TGF-¿ signaling at different stages of prostate cancer progression. A. Specific aims: 1. To determine how miR-205 and miR-31 regulate apoptosis in prostate cancer cells. 2. To investigate the mechanism of down-regulation of miR-205 and miR-31 in advanced prostate cancer. 3. To enhance response to chemotherapy by targeting miR-205 and miR-31. Hypothesis: 1. miR-205 and miR-31 regulate apoptosis in prostate cancer cells. 2. miR-205 and miR-31 are down-regulated by epigenetic silencing in prostate cancers. 3. Targeting miR-205 and miR-31 will enhance response to chemotherapy.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 基质金属蛋白酶（MMP）是癌症治疗的有希望的靶标。然而，最近 MMP抑制剂的临床试验结果令人失望。某些 MMP 可能调节细胞凋亡 信号通路并使肿瘤细胞对凋亡信号敏感。抑制这些 MMP 将 促进肿瘤细胞存活，这种作用可能会抵消抑制肿瘤细胞的有益活性。 转移。基质金属蛋白酶-9 (MMP-9) 表达显着增加 恶性前列腺癌。除了其切割胶原蛋白和基底的能力 作为膜成分，MMP-9 还可裂解并激活多功能细胞因子 TGF-¿。 TGF-¿（在哺乳动物中具有三种同工型：TGF-¿1、Ф2、Ф3）是重要的调节因子 正常和恶性前列腺。 TGF-¿1 在某些前列腺癌中诱导细胞凋亡 细胞中，TGF-¿2 可以阻止细胞凋亡。通过激活 TGF-¿ 的所有三种亚型，MMP-9 可能具有 对前列腺癌细胞凋亡的净影响不同，具体取决于 TGF-¿ 同种型、其受体和下游信号分子的表达状态。有一个关键的 需要确定 MMP-9 对前列腺癌细胞凋亡的影响。据推测， 抑制 MMP-9 对 MMP-9 介导的 TGF-¿ 激活已发生的患者有益。 对癌细胞具有抗凋亡作用。 根据TGF-¿信号机制的状态选择合适的患者可能是 对于未来评估 MMP-9 抑制剂治疗效果的临床试验至关重要。这 本研究的目的是确定 MMP-9 在前列腺癌细胞凋亡中的作用以及如何 MMP-9 对细胞凋亡的影响随着前列腺癌的进展而变化。在 该项目完成后，我们期望阐明MMP-9在细胞凋亡调节中的作用 前列腺癌以及这种作用如何随着 TGF-¿ 信号传导的变化而变化 在前列腺癌进展的不同阶段。 A、具体目标： 1. 确定miR-205和miR-31如何调节前列腺癌细胞的凋亡。 2. 探讨miR-205和miR-31在晚期前列腺癌中下调的机制。 3. 通过靶向miR-205和miR-31来增强对化疗的反应。 假设： 1. miR-205和miR-31调节前列腺癌细胞的凋亡。 2. miR-205 和 miR-31 在前列腺癌中通过表观遗传沉默下调。 3. 靶向miR-205和miR-31将增强对化疗的反应。