Acquisition of a high-performance liquid chromatography system

购置高效液相色谱系统

• 批准号: 9707449
• 负责人: BIN GUO
• 金额: $ 6.41万
• 依托单位: NORTH DAKOTA STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-09-05 至 2020-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9707449
• 关键词: 10 year old; Administrative Supplement; Age; Award; Computer software; Encapsulated; Fluorescence; Funding; Funding Agency; Gel; Gel Chromatography; High Pressure Liquid Chromatography; Laboratories; Maintenance; Mission; Molecular Weight; National Institute of General Medical Sciences; Outcome; Peptides; Pharmaceutical Preparations; Polymers; Public Health; Refractive Indices; Research; Services; System; Time; United States National Institutes of Health; Vesicle; cost; detector; experimental study; fundamental research; human disease; indexing; instrument; nanoparticle; tumor

Project Summary (1R01GM114080 Administrative Supplement) As a part of my current project (R01GM114081), we need to synthesize peptides, conjugate them with polymers, purify the conjugates, and characterize the nanoparticles. Currently, we are completing the studies proposed in the Aim 2 and proceeding to the experiments of Aim 3. However, for the studies to proceed at the desired pace, we need to synthesize the polymers, peptides, the conjugates, purify and characterize them. In addition, we also need a gel-permeation chromatography set-up to characterize the synthesized polymers and the polymer- peptide conjugates. We are using an HPLC system in our laboratory for purifying the synthesized peptides. We are using the HPLC system with a gel-permeation column for determining the molecular weights and the polydispersity indices of the polymers and their peptide conjugates. The instrument is more than ten years old. Due to the age, the instrument is subject to frequent maintenance issues and servicing. The HPLC problems are slowing us on the project, and I am actively looking into funding sources to replace the old HPLC instrument. A new HPLC instrument with fluorescence and refractive index detectors would cost $64,145 from Shimadzu (quotation included in the request). Since our current HPLC system is from Shimadzu, I contacted them for the quotation of a new instrument. We are familiar with the instrument, controls, software, and troubleshooting aspects of the Shimadzu HPLC system. The requested funds would allow us to purchase a new Shimadzu HPLC-GPC combined system for timely-conclusion of the funded project. The instrument would also aid in the competitive renewal of the NIH R01 award.

项目摘要（1 R 01 GM 114080行政补充） 作为我目前项目（R 01 GM 114081）的一部分，我们需要合成肽，将它们与聚合物结合， 纯化缀合物并表征纳米颗粒。目前，我们正在完成 目标2和进行目标3的实验。然而，为了使研究以预期的速度进行， 我们需要合成聚合物、肽、缀合物，纯化并表征它们。另外我们 还需要凝胶渗透色谱装置来表征合成的聚合物和聚合物- 肽缀合物。 我们在实验室中使用HPLC系统来纯化合成的肽。我们用高效液相色谱 具有凝胶渗透柱的系统，用于测定聚合物的分子量和多分散指数。 聚合物和它们的肽缀合物。这台仪器已经有十多年的历史了。由于年龄的原因， 仪器经常受到维护和保养问题的影响。高效液相色谱仪的问题正在拖慢我们 项目，我正在积极寻找资金来源，以取代旧的HPLC仪器。 一个新的高效液相色谱仪与荧光和折射率检测器将花费64,145美元从岛津 （报价包含在请求中）。由于我们目前的HPLC系统来自岛津，我联系了他们， 一种新的工具。我们熟悉仪器、控制、软件和故障排除 Shimadzu HPLC系统的各个方面。这笔资金可以让我们买一辆新的岛津 高效液相色谱-凝胶渗透色谱联用系统，及时完成资助项目。该文书还将有助于 NIH R 01奖的竞争性更新。