CUMC: IMAGING CORE

CUMC：成像核心

• 批准号: 8360029
• 负责人: RICHARD John HALLWORTH
• 金额: $ 2.52万
• 依托单位: UNIVERSITY OF NEBRASKA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8360029
• 关键词: Argon; Cells; Fluorescence; Funding; Grant; Image; Lasers; Life; Microscope; National Center for Research Resources; Nebraska; Oils; Photons; Principal Investigator; Research; Research Infrastructure; Resolution; Resources; Scanning; Source; Spectrum Analysis; System; United States National Institutes of Health; Water; Work; base; cost; fluorophore; functional genomics; multi-photon; research study; structural biology

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. This imaging facility is a new multiuser core that is closely related to the scientific focus in structural biology. The core is based upon the acquisition of LSM 510 METANLO confocal scanning system with three lasers (Argon 458/477/488/514 nm, Green HeNe 543 nm, Red HeNe 633 nm. The confocal is built on a Zeiss AxioPlan 2IE MOT motorized upright microscope, with the following objectives: Plan Neo 40x oil 1.3 numerical aperture (n.a.) with differential interference contrast (DIC); Plan 63x 1.4 n.a. DIC; Achroplan 40x 0.8 n.a. water DIC, for live cell work; C-Apochromat 40x 1.2 n.a. DIC ,for fluorescence correlation spectroscopy; Plan Apo 10x; Plan Neo 20x 0.75 n.a. DIC. Initial funding for the core was through an NSF EPSCoR grant. The Core can image with most fluorophores, in either single-photon or multi-photon modes, with resolution approaching the theoretical limit (222 nm) The META multi-channel system enables separation of fluorophores with closely-spaced emission peaks by linear un-mixing. The types of experiments available far surpass those of conventional high resolution confocal imaging.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 该成像设备是一个新的多用户核心，与结构生物学的科学重点密切相关。 核心是基于LSM 510 METANLO共焦扫描系统的采集，该系统具有三个激光器（氩气458/477/488/514 nm，绿色HeNe 543 nm，红色HeNe 633 nm）。共焦显微镜建立在Zeiss AxioPlan 2 IE MOT电动立式显微镜上，具有以下物镜：Plan Neo 40 x oil 1.3数值孔径（n.a.）带微分干涉对比度（DIC）;平面63 x 1.4 n.a. DIC; Achroplan 40 x 0.8不适用水DIC，用于活细胞工作; C-Apochromat 40 x 1.2 n.a. DIC，用于荧光相关光谱; Plan Apo 10 x; Plan Neo 20 x 0.75 n.a. DIC。 核心的初始资金是通过NSF EPSCoR赠款。 Core可以在单光子或多光子模式下对大多数荧光团进行成像，分辨率接近理论极限（222 nm）。Meta多通道系统通过线性解混，能够分离具有密集发射峰的荧光团。 可用的实验类型远远超过传统的高分辨率共焦成像。