Neuroprosthesis development utilizing afferent neural activity recorded with non-

利用非记录的传入神经活动开发神经假体

• 批准号: 8324058
• 负责人: Timothy M. Bruns
• 金额: $ 5.18万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-01 至 2013-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8324058
• 关键词: Acceleration; Address; Animal Experiments; Animals; Anogenital region; Attention; Back; Calibration; Cells; Chronic; Clinical; Complex; Cutaneous; Development; Devices; Disease; Electric Stimulation; Electrodes; Evaluation; Face; Family Felidae; Fatigue; Feedback; Felis catus; Financial compensation; Future; Goals; Human; Implant; Individual; Institutional Review Boards; Joints; Leg; Limb structure; Location; Locomotion; Lower Extremity; Mechanics; Methods; Microelectrodes; Modeling; Monitor; Motor; Movement; Muscle; Nerve; Nervous system structure; Neurons; Operative Surgical Procedures; Output; Pathway interactions; Patients; Positioning Attribute; Protocols documentation; Research; Route; Sensory; Signal Transduction; Spinal Cord; Spinal Ganglia; Spinal cord injury; Structure; Surface; System; Tactile; Techniques; Technology; Testing; Time; Tissues; Touch sensation; Translations; Update; Vertebral column; Work; afferent nerve; animal data; base; bone; design; human subject; joint mobilization; limb movement; neuronal cell body; neuroprosthesis; relating to nervous system; research clinical testing; research study; response; sensor; sensory feedback; somatosensory; success; technology development

DESCRIPTION (provided by applicant): The goal of this work is the development of a practical, non-penetrating somatosensory neural interface at the level of the dorsal root ganglia (DRG), for use as sensory feedback. The DRG is an ideal location to record somatosensory neural signals which convey body-state information such as tactile and proprioceptive feedback from the limbs. These signals can be used as control signals in closed loop functional electrical stimulation (FES) applications in which the position of a limb is decoded and used to regulate the FES system to adapt to fatigue or the addition of a load or to perform complex, multi-joint movements smoothly. Internal neural interface sensors, such as these may be minimally obtrusive and integrate easily with implanted neuroprostheses while not requiring the large number of sensors or regular donning and calibration that external sensors do. Our lab has shown the ability to decode limb position with high accuracy from recordings of primary afferent neural activity with penetrating microelectrodes inserted in lumbar DRG, and has applied these signals as feedback within closed-loop FES control of the lower limb. However, the efficacy of long-term recordings from these penetrating electrodes has yet to be established, and recordings with penetrating electrodes in humans are challenging to obtain. We hypothesize that recordings from the surface of the DRG may be sufficient for extracting detailed information about limb position and may provide a more practical route for clinical evaluations. Unlike other neural structures, cell bodies are packed closely under the DRG perineum, making it an ideal candidate for obtaining activity from individual cells without using penetrating electrodes. Also, this surface approach may have a higher efficacy in long-term recordings, due to a reduced tissue response, than penetrating electrodes. Recently we showed that recordings from the surface of the DRG, with non-penetrating electrodes, can yield neural signals that can be used to predict the state of the limb, in animal studies. We will evaluate two specific aims in the research planned in this study. Aim 1: In animal experiments, we will evaluate the ability of neural recordings from non-penetrating electrodes on the L6 and L7 DRG to provide functional closed loop control of FES controlled lower limb stepping movements. This study will establish the utility of this approach, by indicating whether a sufficient range of neural activity can be recorded from, to obtain feedback towards functional limb movements. Aim 2: In intraoperative human experiments, we will evaluate the ability of non-penetrating electrodes placed on the surface of exposed DRG to record neural activity that predicts mechanical and vibratory stimulation applied to the lower leg. This study will obtain the first recordings from DRG in humans and demonstrate that we are able to decode human DRG activity. Success in this proposed work will be an important advance towards development of closed-loop neuroprostheses based on this non-penetrating DRG approach and will drive future studies on extended duration animal and human recordings using optimized electrodes.

描述（由申请人提供）： 这项工作的目的是开发在背根神经节（DRG）水平上的实用，非重音的体感神经界面，以用作感觉反馈。 DRG是记录体感神经信号的理想场所，这些神经信号传达了身体状态信息，例如触觉和四肢的本体感受反馈。这些信号可以用作闭环功能电刺激（FES）应用中的控制信号，其中肢体位置被解码并用于调节FES系统以适应疲劳或增加负载或进行复杂的多关节运动。内部神经界面传感器（例如这些传感器）可能是微不足道的，并且可以轻松地与植入的神经假体集成在一起，而不需要大量的传感器或外部传感器的常规don和校准。我们的实验室表明能够从腰部DRG中插入的一级传入神经活动的记录中以高精度来解码肢体位置，并将这些信号应用于下肢的闭环FES控制中。但是，这些穿透电极的长期记录的疗效尚未确定，并且在人类中具有穿透电极的记录很具有挑战性。我们假设来自DRG表面的记录可能足以提取有关肢体位置的详细信息，并且可以为临床评估提供更实际的途径。与其他神经结构不同，细胞体在DRG会阴下方紧密包装，使其成为从不使用穿透电极的情况下从单个细胞中获得活性的理想候选者。同样，由于组织反应降低，这种表面方法在长期记录中可能具有更高的疗效，而不是穿透电极。最近，我们表明，在动物研究中，来自DRG表面的记录可产生可用于预测肢体状态的神经信号。我们将评估本研究计划的研究中的两个具体目标。 AIM 1：在动物实验中，我们将评估L6和L7 DRG上非穿透电极的神经记录的能力，以提供FES控制的下肢踏脚运动的功能闭环控制。这项研究将通过指出是否可以从功能性肢体运动的反馈中记录足够的神经活动来确定这种方法的实用性。 AIM 2：在术中人类实验中，我们将评估放置在暴露DRG表面上的非穿透电极记录神经活动的能力，该神经活动可预测用于下腿上的机械和振动刺激。这项研究将获得人类DRG的第一批录音，并证明我们能够解码人类DRG活动。在这项提出的工作中的成功将是基于这种非穿透DRG方法开发闭环神经植物的重要进步，并将使用优化的电极来推动对延长持续时间动物和人类记录的未来研究。