Daily Experience in Adolescence and Biomarkers of Early Risk for Adult Health

描述（由申请人提供）：尽管要求识别慢性健康状况发展的早期风险因素，但在25岁以下人群中进行的健康生物标志物的纵向研究很少。此外，只有一个最低限度的了解如何成年人健康的早期风险的生物指标发展中的青少年与拉丁美洲和亚洲背景，两个增长最快的种族群体在美国，谁是在不同的风险为不利的健康结果相比，欧洲裔美国人。了解这些过程对于开展与族裔和文化相关的预防工作是必要的。 我们提出了一个3波纵向研究的青少年和他们的照顾者从墨西哥，中国和欧洲的背景，以评估日常经验对成人健康的早期风险的生物指标的影响。我们之所以关注日常经验，是因为需要确定人口调查中确定的全球社会因素，如教育程度低和收入低，在个人日常生活中发挥作用的机制。社会冲突、过度要求、情绪困扰、威胁和睡眠行为等日常经历已被证明与全球风险因素和成年人健康风险的多个生物指标有关。 该项目将包括对青少年及其父母进行深入的行为评估和详细的健康风险生物标志物，以实现以下具体目标：（1）描述青少年时期成人健康早期风险生物指标的发展;（2）评估日常经历在早期健康风险发展中的作用;（3）研究早期健康风险是否存在种族差异;（4）探讨潜在的保护因素在早期健康风险发展中的作用。 当青少年大约15 - 16岁、17 - 18岁和19 - 20岁时，将对大约540对青少年和主要照顾者（每个种族180人）进行评估。每年，青少年和照顾者都将参加访谈，其中包括衡量全球社会因素，如社会经济背景和潜在的保护因素，如社会联系。参与者将使用连续9天的夜间日记清单报告每日体验。将在其中4天的每天4个时间点获得唾液皮质醇，以分析HPA活性，并且参与者将在相同的4天内佩戴腕部活动记录仪以测量客观的睡眠行为。将评估血压、BMI和腰臀比，并获得干血斑用于评估C反应蛋白（CRP）、胆固醇和高密度脂蛋白（HDL）。最后，将通过120个家庭的子样本提供外周血样本，用于评估血浆白细胞介素-6（IL-6）（一种促炎细胞因子），并用于驱动炎症生物学的分子信号传导途径的基因表达分析。 公共卫生相关性：只有一个最低限度的了解如何在拉丁美洲和亚洲背景的青少年中发展成人健康的早期风险的生物指标，这两个增长最快的种族群体在美国，谁是在不同的风险相比，欧洲裔美国人的不良健康结果。对这些人群中青少年的日常生活进行更深入的研究可以确定这些差异的来源以及独特的风险和保护因素。了解这些过程对于开展与族裔和文化相关的预防工作是必要的。

DESCRIPTION (provided by applicant): Despite calls for the identification of early risk factors for the development of chronic health conditions, there has been little longitudinal research on biomarkers of health that has been conducted in persons younger than 25 years of age. In addition, there is only a minimal understanding of how biological indices of early risk for adult health develop among adolescents with Latin American and Asian backgrounds, the two fastest rising ethnic groups in the United States, who are at differential risk for adverse health outcomes as compared to European Americans. An understanding of these processes is necessary for the development of ethnically and culturally relevant prevention efforts. We propose a 3-wave longitudinal study of adolescents and their caregivers from Mexican, Chinese and European backgrounds in order to assess the impact of daily experience on biological indicators of early risk for adult health. Our focus on daily experience stems from the need to identify the mechanisms by which global social factors identified in demographic surveys, such as lower education and income, play themselves out in individuals' daily lives. Daily experiences such as social conflict, excessive demands, emotional distress, threat, and sleep behaviors have been shown to be linked to both global risk factors and multiple biological indices of health risk among adults. The project will include both intensive behavioral assessments and detailed biological markers of health risk from both adolescents and their parents in order to address the following specific aims: (1) describe the development during adolescence of biological indices of early risk for adult health; (2) assess the role of daily experience in the development of early health risk; (3) examine the existence of ethnic disparities in early health risk; and (4) explore the role of potential protective factors in the development of early health risk. Approximately 540 pairs of adolescents and primary caregivers (180 from each ethnic group) will be assessed when the adolescents are approximately 15-16, 17-18, and 19-20 years of age. Each year, both adolescents and caregivers will participate in interviews that include measures of global social factors such as socioeconomic background and potential protective factors such as social connectedness. Participants will report daily experiences using a nightly diary checklist for 9 consecutive days. Salivary cortisol will be obtained at 4 time points each day for 4 of these days in order to analyze HPA activity, and participants will wear wrist actigraphs for the same 4 days to measure objective sleep behaviors. Blood pressure, BMI, and waist/hip ratio will be assessed, and dried blood spots will be obtained for the assessment of c-reactive-protein (CRP), cholesterol, and high density lipoproteins (HDL). Finally, peripheral blood samples will be provided by a subsample of 120 families for the assessment of plasma interleukin-6 (IL-6), a pro-inflammatory cytokine, and for gene expression analyses of molecular signaling pathways driving inflammatory biology. PUBLIC HEALTH RELEVANCE: There is only a minimal understanding of how biological indices of early risk for adult health develop among adolescents with Latin American and Asian backgrounds, the two fastest rising ethnic groups in the United States, who are at differential risk for adverse health outcomes as compared to European American. More in-depth examinations of the daily lives of adolescents from these populations could identify both the sources of these disparities as well as unique risk and protective factors. An understanding of these processes is necessary for the development of ethnically and culturally relevant prevention efforts.