Genomics and Proteomics

鲍尔斯基因组学和蛋白质组学汉农 项目总结(见说明)： CORE D为该计划的成员提供尖端的基因组和蛋白质组工具。CSHL在上一个资金周期中做出了非常重要的机构承诺，以开发大型下一代测序设施，并拥有相当大的能力，由4台HiSeq和12台LLX llLumina测序仪以及太平洋生物科学公司和454的测序仪提供。通过以下方式实现 Core D为计划项目成员提供了一套广泛的下一代测序工具应用程序。这包括RNA-Seq、Chip-Seq和创新的DNA甲基组分析工具。此外，核心D将允许计划成员使用下一代测序工具执行拷贝数变异(CfvIV)、单核苷酸变异(SNV)和其他突变分析。此外，核心将协助研究人员对多路shRNA筛选进行去卷积。核心D还将使利用下一代测序技术在单细胞水平上分析DNA或RNA的项目成为可能。Core D还利用TaqMan和微阵列服务进行实时PCR分析。 Core D雇用了蛋白质组学领域的世界领先者。达里尔·帕平博士。具体服务包括使用SILAC或iTRAQ进行定量蛋白质组谱分析、分析多蛋白质复合体的蛋白质含量以及分析翻译后修饰。 Core D还提供了一名专门的计算科学家，为计划项目成员提供由Core D生成的高通量基因组和蛋白质数据的专家分析。根据需要，将为计划项目成员开发定制工具。 最后，Core D从包括合作人体组织网络在内的可用公共来源为计划项目成员获取人类肿瘤样本和人类癌细胞系，并根据需要分离DNA、RNA或蛋白质。

D: S. Powers Genomics and Proteomics Hannon, Gregory J PROJECT SUMMARY (See instructions): Core D provides cutting edge genomic and proteomic tools to the members of the Program. CSHL has made a very substantial institutional commitment during the last funding cycle to development of a large next-generation sequencing facility, and has considerable capacity provided by four HiSeq and twelve llx lllumina sequencers, along with sequencing machines from Pacific BioSciences and 454. Enabled by this Core D provides as extensive set of next-generation sequencing tools applications for Program Project members. This includes RNA-Seq ,Chip-Seq and innovative DNA methylome analysis tools. In addition Core D will allow program members to perform copy-number variation (CfvIV) and single-nucleotide variation (SNV) and other mutational analysis with the next-generation sequencing tools. In addition the Core will aid investigators in de-convolution of multiplexed shRNA screens. Core D will also enable projects that utilize next-generation sequencing technologies for analyzing DNA or RNA at the single-cell level. Core D also performs real-time PCR analysis with TaqMan and microarray services. Core D employs a world leader in the field of proteomics. Dr. Darryl Pappin. Specific services include quantitative proteome profiling using SILAC or iTRAQ, analysis of the protein content of multiprotein complexes, and analysis of post-translational modifications. Core D also provides a dedicated computational scientist who will provide Program Project members with expert analysis of the high-throughput genomic and proteomic data generated by Core D. Custom tools will be developed for Program Project members where needed. Finally, Core D acquires human tumor samples and human cancer cell lines for Program Project members from available public sources including the Cooperative Human Tissue Network and isolates DNA, RNA, or protein as needed.