Core D

芯

• 批准号: 8744325
• 负责人: Scott Powers
• 金额: $ 42.55万
• 依托单位: COLD SPRING HARBOR LABORATORY
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-01-01 至 2016-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8744325
• 关键词: Biochemistry; Biological Models; Cancer cell line; Cells; ChIP-seq; Commit; Communities; Complex; Cooperative Human Tissue Network; Copy Number Polymorphism; Custom; DNA; DNA Resequencing; Data; Data Set; Development; Epigenetic Process; Equipment; Funding; Future; Galaxy; Gene Expression; Genetic Transcription; Genomics; Goals; Human; Individual; Ions; Laboratories; Messenger RNA; Methods; Molecular Biology; Multiprotein Complexes; NCI Center for Cancer Research; Nucleotides; Post Translational Modification Analysis; Protein Analysis; Proteins; Proteome; Proteomics; RNA; RNA Splicing; Real-Time Systems; Recruitment Activity; Research; Research Personnel; Robotics; Running; Sampling; Scientist; Services; Small Nucleolar RNA; Source; Stem cells; System; Technology; Time; Tissues; Variant; epigenome; epigenomics; high throughput analysis; improved; innovation; instrument; mass spectrometer; member; methylome; next generation sequencing; programs; single cell analysis; small hairpin RNA; success; tool; transcriptome sequencing; tumor

Core D provides cutting edge genomic and proteomic tools to the members of the Program. CSHL has made a very substantial institutional commitment during the last funding cycle to development of a large next-generation sequencing facility, and has considerable capacity provided by four HiSeq and twelve llx lllumina sequencers, along with sequencing machines from Pacific BioSciences and 454. Enabled by this Core D provides as extensive set of next-generation sequencing tools applications for Program Project members. This includes RNA-Seq ,Chip-Seq and innovative DNA methylome analysis tools. In addition. Core D will allow program members to perform copy-number variation (CfvIV) and single-nucleotide variation (SNV) and other mutational analysis with the next-generation sequencing tools. In addition the Core will aid investigators in de-convolution of multiplexed shRNA screens. Core D will also enable projects that utilize next-generation sequencing technologies for analyzing DNA or RNA at the single-cell level. Core D also performs real-time PCR analysis with TaqMan and microarray services. Core D employs a world leader in the field of proteomics. Dr. Darryl Pappin. Specific services include quantitative proteome profiling using SILAC or iTRAQ, analysis of the protein content of multiprotein complexes, and analysis of post-translational modifications. Core D also provides a dedicated computational scientist who will provide Program Project members with expert analysis of the high-throughput genomic and proteomic data generated by Core D. Custom tools will be developed for Program Project members where needed. Finally, Core D acquires human tumor samples and human cancer cell lines for Program Project members from available public sources including the Cooperative Human Tissue Network and isolates DNA, RNA, or protein as needed

核心D为该计划的成员提供尖端的基因组和蛋白质组学工具。CSHL在上一个供资周期中做出了非常实质性的机构承诺，以开发大型下一代测序设施，并通过四台HiSeq和十二台llx lllumina测序仪沿着Pacific BioSciences和454的测序仪提供了相当大的能力。在此基础上，Core D为计划项目成员提供了广泛的下一代测序工具应用程序。这包括RNA-Seq，Chip-Seq和创新的DNA甲基化分析工具。另外。核心D将允许程序成员使用下一代测序工具进行拷贝数变异（CfvIV）和单核苷酸变异（SNV）以及其他突变分析。此外，Core将帮助研究人员对多重shRNA筛选进行去卷积。Core D还将支持利用下一代测序技术在单细胞水平上分析DNA或RNA的项目。Core D还使用TaqMan和微阵列服务进行实时PCR分析。核心D聘请了蛋白质组学领域的世界领先者。达里尔·帕平医生具体服务包括使用SILAC或iTRAQ的定量蛋白质组分析、多蛋白复合物的蛋白质含量分析以及翻译后修饰分析。Core D还提供了一名专门的计算科学家，他将为项目成员提供Core D生成的高通量基因组和蛋白质组数据的专家分析。将在需要时为计划项目成员开发定制工具。最后，核心D从包括合作人类组织网络在内的可用公共来源为计划项目成员获取人类肿瘤样本和人类癌细胞系，并根据需要分离DNA、RNA或蛋白质