Project 3: Molecular/Disease Consequences of Prenatal BPA, PAH Expos. Across Gene

这项建议的目标是确定产前暴露于环境的机制 内分泌干扰物双酚A和多环芳烃的相关水平 多环芳烃(PAHs)对神经生物学、新陈代谢、免疫功能和行为产生长期影响。我们建议 用跨代繁殖技术检测Balb/c小鼠出生前双酚A(口服)和多环芳烃(吸入)的暴露 可以在F1代和F2代孙代中检测影响的设计。这项提议意在 确定产前暴露BPA和PAH对小鼠DNA甲基化和基因表达的不同影响 几种组织(海马体、下丘脑、大脑皮层、脂肪细胞和血液)及其相互关系 分子变化与神经生物学和生理结果。在整个怀孕过程中都会接触到 出生后母婴之间的相互作用将被评估为对后代的潜在调节影响 发展。对男性和女性F1和F2后代的评估将包括体重监测、家庭 社交(PND 30-40)、类焦虑行为(PND 40)和认知功能(PND 44-60)。 肥胖症将在行为测试后进行量化，如果是双酚A暴露，则额外暴露 将包括小组，以检查免疫功能。暴露引起的脑细胞结构改变将 在PND 40的F1和F2后代中被量化。我们将评估驱动这些效应的分子机制。 在胎儿发育(GD19)和成年期(PND 60)的子代组织中。分子的选择 目标将基于从哥伦比亚儿童中心收集的组织分析结果 环境健康(CCCEH)队列，特别关注与神经发育和 肥胖。甲基化分析将涉及亚硫酸氢盐处理的DNA样本与mRNA的焦磷酸测序 通过定量RT-PCR进行分析。总体而言，这些研究旨在确认和验证 CCCEH对双酚A和多环芳烃暴露的人体研究中确定的生物标志物，并确定 血液中的表观遗传变化与脑和脂肪组织中确定的表观遗传变化之间的假设联系 确定可能的机制途径，通过这些途径来调节暴露的长期影响。

The objective of this proposal is to determine the mechanism by which prenatal exposure to environmentally relevant levels of the endocrine disrupting chemicals bisphenol A (BPA) and polycyclic aromatic hydrocarbons (PAHs) exert long-term effects on neurobiology, metabolism, immune function and behavior. We propose to examine prenatal exposure to BPA (oral) and PAH (inhaled) in Balb/c mice using a transgenerational breeding design in which the effects can be examined in Fl offspring and F2 grand-offspring. This proposal intends to determine the distinct effects of prenatal BPA and PAH exposure on DNA methylation and gene expression in several tissues (hippocampus, hypothalamus, brain cortex, adipocytes and blood) and the association of these molecular changes with neurobiological and physiological outcomes. Exposure will occur throughout gestation and postnatal mother-infant interactions will be assessed as a potential modulating influence on offspring development. Assessment of male and female Fl and F2 offspring will include weight monitoring, homecage social interactions (PND 30-40), anxiety-like behavior (PND 40), and cognitive functioning (PND 44-60). Adiposity will be quantified following behavioral testing and in the case of BPA exposure, additional exposure groups will be included to examine immune function. Exposure-induced changes in brain cytoarchitecture will be quantified in Fl and F2 offspring at PND 40. The molecular mechanisms driving these effects will be assessed in offspring tissue during fetal development (GD 19) and in adulthood (PND 60). The selection of molecular targets will be based on the results of analysis of tissue collected from the Columbia Center for Children's Environmental Health (CCCEH) cohort with particular focus on genes involved in neurodevelopment and obesity. Methylation analysis will involve pyrosequencing of bisulphite treated DNA samples with mRNA analysis achieved through quantitative RT-PCR. Overall, these studies are designed to confirm and validate the biomarkers determined in the CCCEH human studies of BPA and PAH exposure and to determine the hypothesized link between epigenetic changes in blood with those determined in brain and adipose tissue to determine the possible mechanistic pathways through which long-term effects of exposure are mediated.