Portable Lab-on-a-chip Flow Cytometer: Prototype and Application Development

描述(由申请人提供)：便携式芯片上实验室流动细胞仪：原型和应用开发NanoSort研究及相关其他项目信息7.项目摘要这里提出的芯片上实验室NanoSort设备促进了第一阶段Beta原型项目的成果。这种设备将适合于商业成功，因为它改善了获得流式细胞术的途径，并降低了分选的成本和复杂性。此外，它还将扩展该设备可以服务的应用，包括用于诊断、预后和个性化医疗的转化性和临床护理点流式细胞术应用。在完成了第一阶段计划中的关键里程碑之后，例如可批量生产的注塑芯片、先进的信号检测和处理以及关键性能指标，我们建议扩展这些能力，以与同类最佳设备竞争。我们这个项目的目标是创造一种设备(NanoSort-1或NS-1)，它将具有改进的性能参数，如使用单个光电倍增管(PMT)进行6色检测、使用低剪应力压电(PZT)致动器进行4路分选以及强大的数字信号处理算法(颜色-空间-时间(COST)实现，允许每秒检测和分拣10,000个事件)。除了这些创新，我们将使用当前良好的制造工艺(CGMP)来制造该设备，以确保该设备将通过UL和CE认证，并可扩展到每年10,000台。我们将咨询加州大学圣迭戈分校和设备制造商(Becton-Dickinson和Life Technologies)的知名研究人员，验证该设备的有效性。 公共卫生相关性：便携式芯片上实验室流式细胞仪：原型和应用开发NanoSort研究及相关其他项目信息8.项目叙述生物医学研究中的重要技术包括基于细胞或颗粒的固有属性进行计数(流式细胞仪)和分离(细胞分类)，目前由非常大(约800磅)和昂贵(约500,000美元)的机器执行，需要专门的空间和工作人员来操作。我们已经发明并申请了三项技术，这三项技术能够以传统仪器的一小部分成本和尺寸开发分选流式细胞仪，使用微流体来引导细胞，使用压电致动器来分选细胞，以及一种名为颜色-时空编码的技术来用一个检测器检测多种颜色(而不是传统仪器中的每种颜色一个检测器)。成本和复杂性的降低使这种新的纳米分选设备对任何研究人员都可用，推动了生物医学的发现。

DESCRIPTION (provided by applicant): Portable Lab-on-a-chip Flow Cytometer: Prototype and Application Development NanoSort RESEARCH & RELATED Other Project Information 7. PROJECT SUMMARY The lab-on-a-chip NanoSort device proposed here advances the achievements of the Phase I Beta Prototype project. This device will be appropriate for commercial success by improving access to and reducing the cost and complexity of sorting flow cytometry. Furthermore, it will expand the applications that this device can serve, including translational and clinical point-of-care flow cytometry applications for diagnostics, prognosis, and personalized medicine. Having completed key milestones in the Phase I program, such as mas- producible injection molded chips, advanced signal detection and processing and key performance metrics, we propose to extend the capabilities to compete with best-in-class devices. Our Aims for this project are to create a device (NanoSort-1 or NS-1) that will have improved performance parameters of 6-color detection with a single photomultiplier tube (PMT), 4-way sorting using low shear stress piezoelectric (PZT) actuators, and a robust digital signal processing algorithm (Color-Space-Time (CoST) implementation that allows 10,000 events per second detection and sorting. In addition to these innovations, we will construct the device using current good manufacturing processes (cGMP) to assure that this device will be UL and CE certified and scalable to 10,000 units per year. We will validate this device with consultation from established researchers at UCSD and device manufactures (Becton-Dickinson and Life Technologies). PUBLIC HEALTH RELEVANCE: Portable Lab-on-a-chip Flow Cytometer: Prototype and Application Development NanoSort RESEARCH & RELATED Other Project Information 8. PROJECT NARRATIVE Important techniques in biomedical research include the counting (flow cytometry) and separation (cell sorting) of cells or particles based on their innate properties, currently performed by very large (~800lbs) and expensive (~$500,000) machines that require dedicated space and staff for their operation. We have invented and patented three techniques that enable the development of a sorting flow cytometer at a fraction of the cost and size of conventional instruments, using microfluidics to channel cells, piezoelectric actuators to sort cells, and a technique called Color-Space-Time coding to detect several colors with a single detector (as opposed to one detector per color in conventional instruments). Reductions in costs and complexity make this new nanosorting device accessible to any researcher, advancing biomedical discovery.