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Universal Precision Tool for Single Cell Capture, Conditioning, and Dispensing

用于单细胞捕获、调节和分配的通用精密工具

基本信息

批准号：
8843898
负责人：
Yu-Hwa Lo
金额：
$ 17.47万
依托单位：
UNIVERSITY OF CALIFORNIA, SAN DIEGO
依托单位国家：
美国
项目类别：
财政年份：
2013
资助国家：
美国
起止时间：
2013-08-01 至 2017-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8843898
关键词：
Address Alkenes Architecture Area Behavior Biocompatible Materials Biological Biological Assay Biology Biomedical Research Cell Communication Cell Count Cell Culture Techniques Cell Cycle Cell Therapy Cell model Cells Cellular biology Characteristics Clinical Research Communicable Diseases Computers Cycloparaffins Detection Devices Disease Progression Dose Effectiveness Electronics Electroporation Environment Freedom Future Genetic Materials Germ Layers Health Hela Cells Hereditary Disease In Vitro Infection Intake Intervention MCF7 cell Maintenance Mammalian Cell Masks Medical Device Medicine Microfluidic Microchips Microfluidics Mus Neoplasm Circulating Cells Noise Performance Pharmaceutical Preparations Pharmacotherapy Physiological Play Polymers Population Preclinical Drug Evaluation Printing Process Production Records Research Research Personnel Role Running Sampling Signal Transduction Specific qualifier value Stem Cell Research Stem cells Stimulus Surface System Technology Testing Time Validation Work base biological systems cancer stem cell cell type conditioning controlled release copolymer cost design digital drug testing electric impedance flexibility fundamental research genetic makeup handheld mobile device human disease in vivo innovation insight instrument laptop lithography operation personalized medicine programs prototype response simulation single cell analysis tissue culture tool tool development trend

项目摘要

DESCRIPTION (provided by applicant): Single cell biology has offered a new path to obtain biological insight that has been masked by the ensemble average from a large cell population. Studying biology at the single-cell level will not only enhance our understanding of the complicated biological mechanisms but also help produce new therapy and drugs to cure human diseases. To support single cell analysis, new tools of extraordinary precision, flexibility, and capability have to be developed. Although microfluidic device has been demonstrated as a highly promising platform for investigating single cells, one serious limit of today's microfluidic single-cell device is that cells in microfluidic environment is very different from cells in standad culture environment and even more different from cells in physiological environment. We propose to develop a universal microfluidic single-cell dispensing and conditioning device to addresses this important limitation. The device has an innovative architecture that integrates different functions, including single cell detection, capturing, conditioning, and release, on a microfluidic platform. The proposed device will operate in a close-loop fashion, requiring no user intervention once the user application is specified, due to the field-programmable-gate-array (FPGA) control and coordination of all the functions together. Besides these unique features, the proposed device can be manufactured at very low cost, fully automated, compatible with industrial standards, and expanded into array format for ultrahigh throughput. The proposed device is intended to become a tool that will be widely used on a daily base by researchers performing fundamental and clinical research benefiting from single cell studies. Since single-cell biology prevails in many areas of biology and medicine, the proposed device is anticipated to satisfy a major need in biomedicine with increasing demands in the future. If developed successfully, the device will facilitate and accelerate discoveries in cancer and stem cell research, new cell therapy, drug screening and testing, infectious and genetic diseases, cell-cell interactions, and cell signaling. The device will also become an invaluable tool for the development of medical devices and assays dealing with rare cell populations such as assays for circulating tumor cells (CTCs), cancer stem cells and personalized medicine.

描述（由申请人提供）：单细胞生物学提供了一种获得生物学见解的新途径，而这种见解被大型细胞群的整体平均值所掩盖。在单细胞水平上研究生物学不仅可以增强我们对复杂生物学机制的理解，而且有助于产生治疗人类疾病的新疗法和药物。为了支持单细胞分析，新工具具有非凡的精度、灵活性，和能力必须得到发展。尽管微流控装置已被证明是一种非常有前途的单细胞研究平台，但当今微流控装置的一个严重限制单细胞装置的特点是微流环境中的细胞与标准培养环境中的细胞有很大不同，更与生理环境中的细胞不同。我们建议开发一种通用的微流体单细胞分配和调节装置来解决这一重要的限制。该设备具有创新的架构，在微流体平台上集成了不同的功能，包括单细胞检测、捕获、调节和释放。由于现场可编程门阵列（FPGA）控制和所有功能的协调，所提出的设备将以闭环方式运行，一旦指定了用户应用程序，就不需要用户干预。除了这些独特的功能之外，所提出的设备可以以非常低的成本制造，完全自动化，与工业标准兼容，并扩展到阵列格式以实现超高吞吐量。拟议的设备旨在成为一种工具，可供研究人员在日常基础上广泛使用，这些研究人员从单细胞研究中受益，进行基础和临床研究。由于单细胞生物学在生物学和医学的许多领域盛行，因此所提出的装置预计将满足生物医学的主要需求，并且未来的需求不断增加。如果开发成功，该设备将促进和加速癌症和干细胞研究、新细胞疗法、药物筛选和测试、传染病和遗传疾病、细胞间相互作用以及细胞信号传导方面的发现。该设备还将成为开发处理稀有细胞群的医疗设备和检测的宝贵工具，例如循环肿瘤细胞 (CTC)、癌症干细胞和个性化医疗的检测。