权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Universal Precision Tool for Single Cell Capture, Conditioning, and Dispensing

用于单细胞捕获、调节和分配的通用精密工具

基本信息

批准号：
8843898
负责人：
Yu-Hwa Lo
金额：
$ 17.47万
依托单位：
UNIVERSITY OF CALIFORNIA, SAN DIEGO
依托单位国家：
美国
项目类别：
财政年份：
2013
资助国家：
美国
起止时间：
2013-08-01 至 2017-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8843898
关键词：
Address Alkenes Architecture Area Behavior Biocompatible Materials Biological Biological Assay Biology Biomedical Research Cell Communication Cell Count Cell Culture Techniques Cell Cycle Cell Therapy Cell model Cells Cellular biology Characteristics Clinical Research Communicable Diseases Computers Cycloparaffins Detection Devices Disease Progression Dose Effectiveness Electronics Electroporation Environment Freedom Future Genetic Materials Germ Layers Health Hela Cells Hereditary Disease In Vitro Infection Intake Intervention MCF7 cell Maintenance Mammalian Cell Masks Medical Device Medicine Microfluidic Microchips Microfluidics Mus Neoplasm Circulating Cells Noise Performance Pharmaceutical Preparations Pharmacotherapy Physiological Play Polymers Population Preclinical Drug Evaluation Printing Process Production Records Research Research Personnel Role Running Sampling Signal Transduction Specific qualifier value Stem Cell Research Stem cells Stimulus Surface System Technology Testing Time Validation Work base biological systems cancer stem cell cell type conditioning controlled release copolymer cost design digital drug testing electric impedance flexibility fundamental research genetic makeup handheld mobile device human disease in vivo innovation insight instrument laptop lithography operation personalized medicine programs prototype response simulation single cell analysis tissue culture tool tool development trend

项目摘要

DESCRIPTION (provided by applicant): Single cell biology has offered a new path to obtain biological insight that has been masked by the ensemble average from a large cell population. Studying biology at the single-cell level will not only enhance our understanding of the complicated biological mechanisms but also help produce new therapy and drugs to cure human diseases. To support single cell analysis, new tools of extraordinary precision, flexibility, and capability have to be developed. Although microfluidic device has been demonstrated as a highly promising platform for investigating single cells, one serious limit of today's microfluidic single-cell device is that cells in microfluidic environment is very different from cells in standad culture environment and even more different from cells in physiological environment. We propose to develop a universal microfluidic single-cell dispensing and conditioning device to addresses this important limitation. The device has an innovative architecture that integrates different functions, including single cell detection, capturing, conditioning, and release, on a microfluidic platform. The proposed device will operate in a close-loop fashion, requiring no user intervention once the user application is specified, due to the field-programmable-gate-array (FPGA) control and coordination of all the functions together. Besides these unique features, the proposed device can be manufactured at very low cost, fully automated, compatible with industrial standards, and expanded into array format for ultrahigh throughput. The proposed device is intended to become a tool that will be widely used on a daily base by researchers performing fundamental and clinical research benefiting from single cell studies. Since single-cell biology prevails in many areas of biology and medicine, the proposed device is anticipated to satisfy a major need in biomedicine with increasing demands in the future. If developed successfully, the device will facilitate and accelerate discoveries in cancer and stem cell research, new cell therapy, drug screening and testing, infectious and genetic diseases, cell-cell interactions, and cell signaling. The device will also become an invaluable tool for the development of medical devices and assays dealing with rare cell populations such as assays for circulating tumor cells (CTCs), cancer stem cells and personalized medicine.

描述（由申请人提供）：单细胞生物学为获得生物学洞察力提供了一条新的途径，这条途径被来自大细胞群体的总体平均值所掩盖。在单细胞水平上研究生物学不仅能增进我们对复杂的生物学机制的理解，而且有助于产生治疗人类疾病的新疗法和新药物。为了支持单细胞分析，新工具非常精确，灵活，