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Cell-Cell Interactions in Developing Retina

视网膜发育中的细胞间相互作用

基本信息

批准号：
8237004
负责人：
Nicholas E Baker
金额：
$ 40.26万
依托单位：
ALBERT EINSTEIN COLLEGE OF MEDICINE
依托单位国家：
美国
项目类别：
财政年份：
1992
资助国家：
美国
起止时间：
1992-08-01 至 2014-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8237004
关键词：
Address Affect Alzheimer&apos s Disease Amyotrophic Lateral Sclerosis Brain Brain Injuries Bypass Cell Communication Cell Count Cell Cycle Cell Cycle Arrest Cell Cycle Progression Cell Cycle Regulation Cell Survival Cells DNA Binding DNA Sequence Development Diabetes Mellitus Dimerization Disease Drosophila melanogaster Drug or chemical Tissue Distribution Environment Eye Development Feedback Future Gene Expression Genes Genetic Genetic Transcription Goals Growth Health Heart Diseases Hematopoiesis Homeostasis Homologous Gene Human Huntington Disease Immune System Diseases In Vitro Lymphoma MHC Class I Genes Macular degeneration Maintenance Malignant Neoplasms Metabolism Methods Molecular Molecular Genetics Myopathy Nerve Degeneration Neural Retina Neuroblastoma Neurodegenerative Disorders Neurons Parkinson Disease Pathway interactions Pattern Photoreceptors Population Prevention Primordium Process Production Proteins Proto-Oncogenes Regulation Research Retina Retinal Retinal Degeneration Retinitis Pigmentosa Role Signal Transduction Specificity Stem cells Stroke Symptoms Tumor Suppressor Genes Up-Regulation Withdrawal Work Yeasts base cardiogenesis cell determination developmental genetics extracellular gene function improved myogenesis nerve stem cell neural patterning neurogenesis neuron loss neuronal replacement neuroprotection novel novel strategies pancreas development precursor cell protein function public health relevance regenerative response self-renewal sex determination stem cell therapy transcription factor tumor

项目摘要

DESCRIPTION (provided by applicant): The research addresses fundamental processes in the formation and survival of neurons, the impulse-conducting cells of the brain and nervous system. One goal is to determine how the decision of stem or precursor cells to become neurons through expression of proneural Class II bHLH genes relies on the earlier expression of genes that create a molecular environment either facilitating or antagonizing the future function of proneural Class II bHLH genes. Genetic and developmental studies of the fruitfly retina and the development of its photoreceptor neurons will be performed to delineate the regulatory network affecting proneural Class II bHLH genes. Molecular genetic studies will identify the specific DNA sequences that are responsible for gene expression and which therefore influence where and when neuronal photoreceptor cells are formed during eye development. A further goal is to determine how the genes that regulate neuronal determination also affect growth and the cell cycle. Genetic and molecular approaches will be taken to elucidate these connections between neurogenesis, growth, and cell cycle arrest, and which are perturbed in cancers and neurodegenerative diseases. PUBLIC HEALTH RELEVANCE: Neuronal deficits underlie common, serious health issues including retinitis pigmentosa, macular degeneration, stroke, Alzheimer's disease, Parkinson's Disease, Huntington's disease, and Amyotrophic Lateral Sclerosis. Replacing or limiting neuronal loss in these conditions would significantly improve or reverse symptoms. Our studies will suggest new approaches to neuroprotection and regenerative approaches involving proliferation and specification of replacement neurons.

描述（由申请人提供）：该研究涉及神经元、大脑和神经系统的脉冲传导细胞的形成和存活的基本过程。一个目标是确定干细胞或前体细胞通过表达原神经 II 类 bHLH 基因而成为神经元的决定如何依赖于基因的早期表达，这些基因创建了促进或拮抗原神经 II 类 bHLH 基因未来功能的分子环境。将进行果蝇视网膜及其感光神经元发育的遗传和发育研究，以描绘影响原神经 II 类 bHLH 基因的调节网络。分子遗传学研究将确定负责基因表达的特定 DNA 序列，从而影响眼睛发育过程中神经元感光细胞形成的地点和时间。进一步的目标是确定调节神经元决定的基因如何影响生长和细胞周期。将采用遗传和分子方法来阐明神经发生、生长和细胞周期停滞之间的这些联系，以及这些联系在癌症和神经退行性疾病中受到干扰。公共健康相关性：神经元缺陷是常见严重健康问题的根源，包括色素性视网膜炎、黄斑变性、中风、阿尔茨海默病、帕金森病、亨廷顿病和肌萎缩侧索硬化症。在这些情况下替代或限制神经元损失将显着改善或逆转症状。我们的研究将提出神经保护和再生方法的新方法，涉及替代神经元的增殖和规范。