Michigan Lupus Epidemiology & Surveillance (MILES) Program Longitudinal Cohort

描述(由申请人提供)：项目摘要/摘要本提案旨在通过创建和维护Miles纵向队列和生物库，将密歇根狼疮流行病学和监测(Miles)计划从接近完成的公共卫生监测阶段扩展到研究阶段，其中将包括现有监测登记中的狼疮病例和基于人口的控制。首要目标将是前瞻性地收集数据和生物标本，以便进行与狼疮临床病程相关的全面调查，包括疾病发生和发展及其并存的风险因素。所有参与者的详细数据收集将包括与社会人口统计、健康/健康、身体机能、环境暴露、遗传有关的一些衡量标准，对于女性参与者，将包括一些与妇女健康有关的项目。将为SLE患者评估针对狼疮的具体措施，包括疾病活动指数。生物库将包括血清、血浆、PBMCs、DNA(从未分离的PBMCs和淋巴细胞亚群中分离出来)、头发和脚趾甲切片。将收集育龄女性参与者的阴道拭子，以便进行HPV DNA分析。与狼疮病因学和共病相关的三个不同的研究模块被整合在一起，主题具体涉及表观遗传学、HPV感染和中枢介导的疼痛。生物库标本将储存在适当的条件下，以便对疾病和暴露的生物标记物进行基因分型和未来的调查。重点将放在提供执行当代免疫分析的能力，包括相关淋巴细胞亚群的DNA甲基化。最后，这项建议将支持使用免疫芯片进行单核苷酸多态(SNP)基因分型。因此，Miles Cohort将成为一个强大的平台，用于正在进行的和未来与狼疮及其并发症的全谱相关的研究调查。

DESCRIPTION (provided by applicant): Project Summary/Abstract This proposal is to extend the Michigan Lupus Epidemiology & Surveillance (MILES) Program beyond the nearly completed public health surveillance phase to a research phase, by creating and maintaining the MILES Longitudinal Cohort & Biobank, which will include both lupus cases from the existing surveillance registry and population-based controls. The overarching goal will be to prospectively collect data and biospecimens that will allow for comprehensive investigations related to the clinical course of lupus, including risk factors for both the onset and progression of disease and its comorbidities. Detailed data collection for all participants will include a number of measures related to sociodemographics, health/wellness, physical functioning, environmental exposures, genetics, and for female participants, a number of items related to women's health. Lupus-specific measures, including disease activity indices, will be assessed for the SLE patients. The biobank will include serum, plasma, PBMCs, DNA (isolated both from unfractionated PBMCs and lymphocyte subsets), hair and toenail clippings. Vaginal swabs for female participants of reproductive age will be collected, allowing for HPV DNA profiling. Three distinct research modules related to lupus etiology and comorbidities are integrated, with the topics specifically dealing with epigenetics, HPV infection, and centrally-mediated pain. Biobank specimens will be stored in appropriate conditions to allow for genotyping and future investigation of biomarkers of both disease and exposure. An emphasis will be placed on providing the capability to perform contemporary immunoassays, including DNA methylation of relevant lymphocyte subsets. Lastly, this proposal will support the performance of single nucleotide polymorphisms (SNP) genotyping using the ImmunoChip. The MILES Cohort will thus serve as a robust platform for ongoing and future research investigations that are relevant to the full spectrum of lupus and its complications.