Michigan Lupus Epidemiology & Surveillance (MILES) Program Longitudinal Cohort

本提案旨在通过创建和维护MILES纵向队列和生物库，将密歇根狼疮流行病学和监测（MILES）项目从即将完成的公共卫生监测阶段扩展到研究阶段，该项目将包括现有监测登记处的狼疮病例和基于人群的对照。总体目标将是前瞻性地收集数据和生物标本，以便对狼疮的临床过程进行全面调查，包括疾病发病和进展及其合并症的危险因素。为所有参与者收集的详细数据将包括与社会人口统计学、健康/保健、身体机能、环境暴露、遗传学有关的一些措施，并为女性参与者收集与妇女健康有关的一些项目。狼疮特异性措施，包括疾病活动指数，将评估SLE患者。生物库将包括血清、血浆、PBMCs、DNA（从未分离的PBMCs和淋巴细胞亚群中分离）、头发和脚趾甲剪发。将收集育龄女性参与者的阴道拭子，以便进行HPV DNA分析。与狼疮病因学和合并症相关的三个不同的研究模块被整合，主题具体涉及表观遗传学，HPV感染和中枢介导的疼痛。生物库标本将储存在适当的条件下，以便进行基因分型和未来对疾病和暴露的生物标志物的研究。重点将放在提供进行当代免疫测定的能力，包括相关淋巴细胞亚群的DNA甲基化。最后，该建议将支持使用免疫芯片进行单核苷酸多态性（SNP）基因分型的性能。因此，MILES队列将作为一个强大的平台，用于与狼疮及其并发症的全谱相关的正在进行和未来的研究调查。

DESCRIPTION (provided by applicant): Project Summary/Abstract This proposal is to extend the Michigan Lupus Epidemiology & Surveillance (MILES) Program beyond the nearly completed public health surveillance phase to a research phase, by creating and maintaining the MILES Longitudinal Cohort & Biobank, which will include both lupus cases from the existing surveillance registry and population-based controls. The overarching goal will be to prospectively collect data and biospecimens that will allow for comprehensive investigations related to the clinical course of lupus, including risk factors for both the onset and progression of disease and its comorbidities. Detailed data collection for all participants will include a number of measures related to sociodemographics, health/wellness, physical functioning, environmental exposures, genetics, and for female participants, a number of items related to women's health. Lupus-specific measures, including disease activity indices, will be assessed for the SLE patients. The biobank will include serum, plasma, PBMCs, DNA (isolated both from unfractionated PBMCs and lymphocyte subsets), hair and toenail clippings. Vaginal swabs for female participants of reproductive age will be collected, allowing for HPV DNA profiling. Three distinct research modules related to lupus etiology and comorbidities are integrated, with the topics specifically dealing with epigenetics, HPV infection, and centrally-mediated pain. Biobank specimens will be stored in appropriate conditions to allow for genotyping and future investigation of biomarkers of both disease and exposure. An emphasis will be placed on providing the capability to perform contemporary immunoassays, including DNA methylation of relevant lymphocyte subsets. Lastly, this proposal will support the performance of single nucleotide polymorphisms (SNP) genotyping using the ImmunoChip. The MILES Cohort will thus serve as a robust platform for ongoing and future research investigations that are relevant to the full spectrum of lupus and its complications.