Michigan Lupus Epidemiology and Surveillance (MILES) Program Longitudinal Cohort & Biobank

项目总结/摘要 这项建议是继续纵向随访狼疮病例和控制在人口为基础的 密歇根州狼疮流行病学和监测（MILES）计划队列和生物库。总体目标是 前瞻性地收集数据和生物样本，以便对风险因素进行全面调查 与狼疮和疾病进展有关，以及潜在的差异。我们目前正处于第三波 队列研究访视。详细的数据收集包括与四个核心内容领域有关的措施： 自然史、治疗、医疗保健可及性和差异。最先进的生物库包括整个 血液、血清、血浆、DNA/RNA（来自淋巴细胞亚群）、尿液、毛发和脚趾甲剪下物。生物库 在适当的条件下处理和储存样本，以提供进行分析的能力 包括当代免疫测定、疾病和暴露的生物标志物、基因分型和DNA 相关淋巴细胞亚群的甲基化。MILES队列和生物库的主要专题领域 旨在支持包括环境和营养流行病学，表观遗传学和卫生服务， research.因此，MILES队列将成为正在进行和未来研究的强大平台 与狼疮及其并发症的全谱相关的调查。

PROJECT SUMMARY/ABSTRACT This proposal is to continue longitudinal follow-up of lupus cases and controls in the population-based Michigan Lupus Epidemiology & Surveillance (MILES) Program Cohort and Biobank. The overarching goal is to prospectively collect data and biospecimens that will allow for comprehensive investigations of risk factors related to lupus and disease progression, as well as underlying disparities. We are currently in a third wave of cohort study visits. Detailed data collection encompasses measures related to four core content areas: natural history, treatment, healthcare access and disparities. The state-of-the-art biobank includes whole blood, serum, plasma, DNA/RNA (from lymphocyte subsets), urine, hair, and toenail clippings. Biobank specimens are processed and stored in appropriate conditions to provide the capacity to perform assays including contemporary immunoassays, biomarkers of disease and exposures, genotyping, and DNA methylation of relevant lymphocyte subsets. Major thematic areas that the MILES Cohort & Biobank is designed to support include environmental and nutritional epidemiology, epigenetics, and health services research. The MILES Cohort will thus serve as a robust platform for ongoing and future research investigations that are relevant to the full spectrum of lupus and its complications.