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Constructing a Conditional Slc7a11 (xCT) Null Mouse

构建条件 Slc7a11 (xCT) 空鼠标

基本信息

批准号：
8302237
负责人：
SANDRA J HEWETT
金额：
$ 25.9万
依托单位：
SYRACUSE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2011
资助国家：
美国
起止时间：
2011-07-15 至 2015-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8302237
关键词：
Alleles Amino Acid Transporter Animal Model Antioxidants Astrocytes Behavioral Brain Brain Injuries Cell Culture Techniques Cell membrane Cells Cerebral Ischemia Chemosensitization Couples Cyst Cysteine Cystine Development Economic Burden Emotional Environment Enzymes Excitatory Amino Acid Receptors Exons Free Radicals Gene Expression Generations Genes Genetic Transcription Glutamate Receptor Glutamate Transporter Glutamates Glutathione Glycine Goals Health Hour Hypoxia Inflammatory Injury Interleukin-12 Interleukins Invaded Ischemic Penumbra Knock-out Knockout Mice Laboratories Lead Light Literature Maintenance Mediating Metabolism Microglia Morbidity - disease rate Mus Mutant Strains Mice Nerve Degeneration Neuronal Injury Neurons Organism Oxidants Oxidative Stress Play Prevention Process Production Proteins Public Health Recycling Role Signal Transduction Site Stroke Substrate Specificity System Tissues Transgenic Mice Translations antiporter cell type cofactor cytokine design driving force extracellular glutathione peroxidase in vitro Model in vivo knockout animal loss of function loss of function mutation new therapeutic target prevent stoichiometry uptake

项目摘要

DESCRIPTION (provided by applicant): Injury to the brain caused by cerebral ischemia is a major public health concern. Studies have determined that the brain damage associated with cerebral ischemia is mediated by over-stimulation of excitatory amino acid receptors, oxidative stress, as well as inflammatory factors. Our laboratory demonstrated - using an in vitro model of the ischemic penumbra - that astrocyte-mediated alterations in system xc- (cystine/glutamate antiporter) contribute to the development and progression of inflammatory (IL-12-mediated) hypoxic neuronal injury. Thus, we believe that system xc- has the potential to be a novel therapeutic target for stroke. However, to validate this hypothesis, our results must be confirmed in vivo. Mice harboring a natural loss of function mutation in the Slc7a11 (sut) gene - which encodes for xCT, the light chain dictating substrate specificity in system xc- - and xCT null mutant mice have been described in the literature. However, these are global knockouts. To date no tissue-specific knockout for this allele exists. Hence, the overall goal of this project is to develop (Aim 1) and characterize (Aim 2) an astrocyte-specific conditional knockout mouse of the Slc7a11 gene for the ultimate use in in vivo.

描述（由申请人提供）：脑缺血引起的脑损伤是一个主要的公共卫生问题。研究已经确定与脑缺血相关的脑损伤是由兴奋性氨基酸受体的过度刺激、氧化应激以及炎症因子介导的。我们的实验室证明-使用缺血半暗带的体外模型-星形胶质细胞介导的系统xc-（胱氨酸/谷氨酸反向转运蛋白）的改变有助于炎症（IL-12介导的）缺氧神经元损伤的发展和进展。因此，我们相信系统xc-有可能成为一个新的治疗中风的目标。然而，为了验证这一假设，我们的结果必须在体内得到证实。在文献中描述了在Slc 7a 11（sut）基因中携带自然功能缺失突变的小鼠-其编码xCT，在系统xc中指示底物特异性的轻链-和xCT无效突变小鼠。然而，这些都是全球淘汰赛。到目前为止，还不存在针对该等位基因的组织特异性敲除。因此，本项目的总体目标是开发（目标1）和表征（目标2）星形胶质细胞特异性条件性敲除小鼠的Slc 7a 11基因的最终体内使用。