Purchase of AB Sciex QTRAP 5500 System

购买 AB Sciex QTRAP 5500 系统

• 批准号: 8246878
• 负责人: PAPASANI V SUBBAIAH
• 金额: $ 43.97万
• 依托单位: UNIVERSITY OF ILLINOIS AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-06-01 至 2013-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8246878
• 关键词: Biological; Cardiovascular Diseases; Chicago; Coupled; Cultured Cells; Development; Diabetes Mellitus; Disease; Equipment; Faculty; Funding; High Pressure Liquid Chromatography; Human; Illinois; Lipids; Liquid substance; Lung diseases; Measurement; Methods; Minor; Molecular; Obesity; Participant; Peptides; Pharmaceutical Preparations; Phospholipids; Plasma; Productivity; Proteins; Research Personnel; Respiration Disorders; Second Messenger Systems; Signal Pathway; Signal Transduction; Site; Sphingolipids; System; Tissues; Translational Research; United States National Institutes of Health; Universities; clinically relevant; instrument; liquid chromatography mass spectrometry; mass spectrometer; novel; operation; second messenger

DESCRIPTION (provided by applicant): This proposal, from a group of established investigators at the University of Illinois at Chicago (UIC), is for the purchase of an LC/MS/MS equipment, fully dedicated to the analysis of various types of lipids and lipid-derived second messengers involved in pathobiology of several human disorders and diseases. Funds are requested for the purchase of an AB Sciex QTRAP 5500 mass spectrometer coupled to an Agilent HPLC system. Participants in this application include13 major users and 8 minor users who are active NIH-supported investigators, supported by about 50 R01 or P01 projects, of which at least 30 will be directly benefited by the instrument. These investigators are engaged in several basic and clinically relevant studies, including cell signaling pathways, cardiovascular diseases, diabetes, obesity, and pulmonary and respiratory disorders, and are in need of a dedicated instrument to characterize and quantify various molecular species of phospholipids, sphingolipids, and neutral lipids in tissues, plasma, biological fluids and cultured cells. Further development of new and more sensitive measurement of bioactive lipids requires on-site availability of state-of-the-art mass spectrometer. The LC/MS instruments that are currently available and in operation at UIC are either not suitable for this purpose, or being utilized fully for other types of analyses, such as proteins, peptides and drug metabolites. Therefore, availability of a state-of-the-art dedicated equipment for analysis of various types of lipids, lipd-derived second messengers and bioactive phospho- and sphingo-lipids will enable the currently NIH funded and non-funded investigators to take advantage of the novel lipidomic methods recently developed by the UIC faculty, as well as by others, towards enhancing their productivity and ability to conduct cutting edge translational research. PUBLIC HEALTH RELEVANCE: Lipids are an important and diverse group of fat molecules that are implicated in several human diseases, including heart and lung diseases, obesity, cancer, diabetes, and inflammatory diseases. The investigators in this application are engaged in several clinically relevant projects that require sophisticated lipid analysis. The knowledge gained from these studies could provide valuable tools for improved diagnosis and treatment of several of the above diseases.

描述(由申请人提供)：这份提案由伊利诺伊大学芝加哥分校(UIC)的一组资深研究人员提出，目的是购买LC/MS/MS设备，完全致力于分析涉及几种人类疾病和疾病的病理生物学中的各种类型的脂质和脂类衍生的第二信使。需要资金用于购买AB Sciex QTRAP 5500质谱仪，该质谱仪与安捷伦高效液相色谱系统相耦合。这项申请的参与者包括13个主要用户和8个次要用户，他们是NIH支持的活跃调查人员，得到了大约50个R01或P01项目的支持，其中至少30个项目将直接受益于该工具。这些研究人员参与了几项基础和临床相关的研究，包括细胞信号通路、心血管疾病、糖尿病、肥胖以及肺和呼吸疾病，并需要一种专用仪器来表征和量化组织、血浆、生物液和培养细胞中各种磷脂、鞘磷脂和中性脂的分子种类。进一步开发新的、更灵敏的生物活性脂类测量方法需要现场使用最先进的质谱仪。UIC现有的和正在运行的LC/MS仪器要么不适合用于这一目的，要么被充分利用 用于其他类型的分析，如蛋白质、多肽和药物代谢物。因此，有了最先进的专用设备来分析各种类型的脂类、脂类衍生的第二信使和生物活性的磷脂和鞘氨醇，将使目前由NIH资助和非资助的研究人员能够利用UIC教员以及其他人最近开发的新的脂体组学方法，以提高他们的生产力和进行尖端翻译研究的能力。 与公共健康相关：脂类是一组重要且多样的脂肪分子，与多种人类疾病有关，包括心肺疾病、肥胖症、癌症、糖尿病和炎症性疾病。这一应用中的研究人员参与了几个临床相关的项目，这些项目需要复杂的血脂分析。从这些研究中获得的知识可以为改进上述几种疾病的诊断和治疗提供宝贵的工具。