Purchase of AB Sciex QTRAP 5500 System

描述（由申请人提供）：该提案来自伊利诺伊大学芝加哥分校（UIC）的一组已建立的研究人员，旨在购买LC/MS/MS设备，完全致力于分析多种类型的脂质和脂质衍生的第二信使，这些脂质和脂质衍生的第二信使涉及多种人类疾病和疾病的病理生物学。请求提供资金用于购买一台与Agilent HPLC系统连接的AB Sciex QTRAP 5500质谱仪。本次申请的参与者包括13名主要用户和8名次要用户，他们是NIH支持的活跃研究者，得到约50个R 01或P01项目的支持，其中至少30个项目将直接受益于该仪器。这些研究人员从事多项基础和临床相关研究，包括细胞信号传导途径、心血管疾病、糖尿病、肥胖症以及肺部和呼吸系统疾病，并且需要专用仪器来表征和定量组织、血浆、生物液体和培养细胞中磷脂、鞘脂和中性脂质的各种分子种类。进一步开发新的和更灵敏的生物活性脂质测量需要现场提供最先进的质谱仪。UIC目前可用和正在运行的LC/MS仪器要么不适合此目的，要么正在充分利用 用于其他类型的分析，如蛋白质、肽和药物代谢物。因此，用于分析各种类型脂质，脂质衍生的第二信使和生物活性磷酸和鞘脂的最先进的专用设备的可用性将使目前NIH资助和非资助的研究人员能够利用UIC教师最近开发的新型脂质组学方法，以及其他人，提高他们的生产力和进行尖端转化研究的能力。 公共卫生相关性：脂质是一组重要且多样的脂肪分子，其与几种人类疾病有关，包括心脏和肺部疾病、肥胖症、癌症、糖尿病和炎性疾病。本申请中的研究人员参与了几个需要复杂脂质分析的临床相关项目。从这些研究中获得的知识可以为改善上述几种疾病的诊断和治疗提供有价值的工具。

DESCRIPTION (provided by applicant): This proposal, from a group of established investigators at the University of Illinois at Chicago (UIC), is for the purchase of an LC/MS/MS equipment, fully dedicated to the analysis of various types of lipids and lipid-derived second messengers involved in pathobiology of several human disorders and diseases. Funds are requested for the purchase of an AB Sciex QTRAP 5500 mass spectrometer coupled to an Agilent HPLC system. Participants in this application include13 major users and 8 minor users who are active NIH-supported investigators, supported by about 50 R01 or P01 projects, of which at least 30 will be directly benefited by the instrument. These investigators are engaged in several basic and clinically relevant studies, including cell signaling pathways, cardiovascular diseases, diabetes, obesity, and pulmonary and respiratory disorders, and are in need of a dedicated instrument to characterize and quantify various molecular species of phospholipids, sphingolipids, and neutral lipids in tissues, plasma, biological fluids and cultured cells. Further development of new and more sensitive measurement of bioactive lipids requires on-site availability of state-of-the-art mass spectrometer. The LC/MS instruments that are currently available and in operation at UIC are either not suitable for this purpose, or being utilized fully for other types of analyses, such as proteins, peptides and drug metabolites. Therefore, availability of a state-of-the-art dedicated equipment for analysis of various types of lipids, lipd-derived second messengers and bioactive phospho- and sphingo-lipids will enable the currently NIH funded and non-funded investigators to take advantage of the novel lipidomic methods recently developed by the UIC faculty, as well as by others, towards enhancing their productivity and ability to conduct cutting edge translational research. PUBLIC HEALTH RELEVANCE: Lipids are an important and diverse group of fat molecules that are implicated in several human diseases, including heart and lung diseases, obesity, cancer, diabetes, and inflammatory diseases. The investigators in this application are engaged in several clinically relevant projects that require sophisticated lipid analysis. The knowledge gained from these studies could provide valuable tools for improved diagnosis and treatment of several of the above diseases.