Nikon A1RSI Confocal Microscope

描述（由申请人提供）：这是克利夫兰州立大学（CSU）的一个研究团队提交的共享仪器S10申请，要求使用Nikon A1共聚焦显微镜。CSU目前没有共聚焦显微镜。事实上，目前有一个单一的荧光显微镜，这是旧的，有限的使用和不共焦，由所有研究人员共享。所要求的设备的主要用户将是NIH资助的教师，主要来自两个部门，但都属于新成立的健康和疾病基因调控中心（“GRHD”）。次要用户将包括其他成员，其中大多数也有较小的NIH赠款和非NIH资金。总的来说，这是一个小而高度活跃的研究人员群体，大多处于相对早期的职业生涯阶段，被誉为各自领域的新兴领导者。随着GRHD研究项目的进展和发展，我们感到对高分辨率显微镜的明确需求，与校园内缺乏这种设施形成鲜明对比。 GRHD的集体研究领域，受益于显微镜，将至少包括：翻译和核糖体外L13 a甲基化的调节;锥虫端粒复合体的调节;内皮细胞药物递送;减数分裂过程中的染色体结构和重组;昼夜节律钟的位置和功能;弓形虫-宿主线粒体相互作用; RNA病毒对先天免疫的调节;巨核细胞和多能干细胞的分化;小RNA生物发生和功能。虽然细节不同，但这些研究人员的总体目标是了解特定蛋白质的亚细胞定位如何调节与心血管疾病，感染性疾病，先天免疫，衰老和癌症相关的细胞和致病过程。为了实现这些项目的全部潜力，绝对需要能够自动聚焦，延时成像和分离固定和活细胞中的多个荧光团的共聚焦显微镜，本提案中概述了这一点。另一个好处是，这所公立大学将加强学生的培训和教育，包括研究生和研究生。 为了补充NIH的预算，CSU和科学与健康学院的各个办公室（系主任，学院院长和研究副总裁）将提供适当的空间，支持训练有素的人员操作显微镜，并在需要时购买延长服务合同。 公共卫生相关性：尼康A1共聚焦显微镜，在这个共享仪器应用程序中要求，将加强属于新成立的健康和疾病基因调控中心在克利夫兰州立大学的一个强大的研究团队的研究。受益于显微镜的多个研究领域将包括心血管和传染病，先天性疾病，衰老和癌症。

DESCRIPTION (provided by applicant): This is a Shared Instrumentation S10 application from a team of investigators at Cleveland State University (CSU), requesting a Nikon A1 Confocal Microscope. There is no confocal microscope in CSU at present. In fact, there is currently a single fluorescence microscope, which is old, limited-use and not confocal, shared by all research faculty. The major users of the requested equipment will be NIH-funded Faculty members, drawn mainly from two Departments, but all belonging to the newly created Center for Gene Regulation in Health and Disease ("GRHD"). Minor users will include other members, most of whom also have smaller NIH grants and non-NIH funding. Together, this is a small but highly active group of researchers, mostly in relatively early career stages, acclaimed as rising leaders in their respective fields. As the research projects of GRHD progressed and evolved, we felt a clear need for high-resolution microscopy, contrasted by the lack of such a facility on campus. The collective research areas of GRHD, benefiting from the microscope, will minimally include: Regulation of translation and rRNA methylation by extra-ribosomal L13a; Regulation of Trypanosome telomere complex; Endothelial cell drug delivery; Chromosome structure and recombination during meiosis; Circadian clock location and function; Toxoplasma-host mitochondria interaction; Regulation of innate immunity by RNA viruses; Differentiation of megakaryocytes and pluripotent stem cells; Small RNA biogenesis and function. Although diverse in detail, the overall Aim of these investigators is to understand how the subcellular localization of specific proteins regulate cellular and pathogenic processes, relevant to cardiovascular disorders, infectious diseases, innate immunity, aging, and cancer. A confocal microscope capable of autofocus, time-lapse imaging and segregating multiple fluorophores in both fixed and living cells is absolutely needed to realize the full potential of these projects, a outlined within this proposal. An added benefit will be the enhancement of student training and education at both under- and post-graduate levels in this public University. To supplement the NIH budget, the various offices of CSU and the College of Sciences and Health Professions (Department Chairs, Dean of College, and Vice President of Research) will provide appropriate space, support trained personnel to operate the microscope, and purchase an extended service contract as and when needed. PUBLIC HEALTH RELEVANCE: The Nikon A1 Confocal Microscope, requested in this Shared Instrumentation application, will enhance the research of a strong team of investigators belonging to the newly created Center for Gene Regulation in Health and Disease at Cleveland State University. The multiple research areas benefiting from the microscope will include cardiovascular and infectious diseases, inborn disorders, aging and cancer.