Development of an SPF Macaca nemestrina Breeding Colony

这项重新提交的动物资源拨款续期提案要求提供五年的资金，以继续支持约翰霍普金斯大学于2006年9月21日首次资助的无特定病原体(SPF)群的猪尾猕猴(Macaca Nomestrina)。它有以下资源部分和研究部分的目标： A.与资源构成相关的目标： (1)扩大目前SPF繁殖群体的规模，以增加可供繁殖的雌性和可出售给约翰·霍普金斯大学和其他研究机构的NIH资助的研究人员的雄性数量。(2)定期检测脑炎疱疹病毒1型(B病毒)、猿猴免疫缺陷病毒(SIV)、猿猴逆转录病毒(SRV)和猿猴T淋巴细胞营养病毒(STLV)4种病原体，以维持菌落的SPF状态。目前，该殖民地目前的所有成员都一再被检测出这些病原体呈阴性。(3)保存所有动物的系谱和健康数据。这些数据可以在群体中的所有动物上获得，并将随着群体的增长而扩大。 B.研究部分的目的：扩大该物种现有的遗传数据，以a)增加该物种的研究效用和b)协助该种群的疾病管理。这将通过三个研究目标来实现。 (1)通过MHC-I类基因分型，对猪尾猕猴免疫遗传学进行详细研究。 (2)利用单核苷酸多态(SNP)遗传标记在整个猪尾猕猴基因组中定位，对猪尾猕猴的遗传特性进行研究。 (3)确定MHC I类等位基因表达或SNPs是否与a)SIV相关疾病结局和b)自然发生的猪尾猕猴小肠结肠炎相关。

This resubmission of our animal resource grant renewal proposal requests five years of funding to continue support for a Specific Pathogen Free (SPF) colony of pig-tailed macaques (Macaca nemestrina) at the Johns Hopkins University that was first funded on September 21, 2006. It has the following resource component and research component aims: A. Resource Component Related Aims: (1) To expand the size of the current SPF breeding colony to increase the numbers of females for breeding and males available for sale to NIH funded investigators both within Johns Hopkins University and at other research institutions. (2) To maintain the SPF status of the colony by regular serologic and PCR testing for 4 viral agents: Cercopithecine herpesvirus 1 (B virus), Simian Immunodeficiency Virus (SIV), Simian Retrovirus (SRV) and Simian T-lymphotrophic Virus (STLV). At present all of the current members of the colony have repeatedly tested negative for these agents. (3) To maintain pedigree and health data on all animals. This data is available on all of the animals in the colony and will be expanded as the colony grows. B. Research Component Aims: To expand the genetic data available for this species to a) increase the research utility of the species and b) assist in disease management of the colony. This will be done through three research aims. (1) To characterize pig-tailed macaque immunogenetics in detail via MHC class I genotyping. (2) To characterize pig-tailed macaque genetics by single nucleotide polymorphism (SNP) genetic marker mapping throughout the pig-tailed macaque genome. (3) To determine whether either MHC class I allele expression or SNPs are associated with a) SIV-related disease outcomes and b) naturally occurring enterocolitis in pig-tailed macaques.