Development of an SPF Macaca nemestrina Breeding Colony

我们的动物资源补助金更新提案的重新提交要求五年的资金，以继续支持在约翰霍普金斯大学的猪尾猕猴（Macaca nemestrina）的无特定病原体（SPF）殖民地，该殖民地于2006年9月21日首次获得资助。它有以下资源部分和研究部分目标： A.资源组成部分相关目标： (1)为了扩大当前SPF繁殖群体的规模，以增加用于繁殖的雌性和可供销售给NIH的雄性数量，约翰霍普金斯大学和其他研究机构的研究人员都提供了资助。(2)通过定期血清学和PCR检测4种病毒因子（猴疱疹病毒1型（B病毒）、猴免疫缺陷病毒（SIV）、猴逆转录病毒（SRV）和猴T淋巴细胞病毒（STLV）），维持殖民地的SPF状态。目前，殖民地的所有现有成员都多次对这些病原体进行了阴性检测。(3)保存所有动物的系谱和健康数据。该数据适用于殖民地中的所有动物，并将随着殖民地的增长而扩展。 B。研究组成部分的目的：扩大该物种的遗传数据，以a）增加该物种的研究效用和B）协助殖民地的疾病管理。这将通过三个研究目标来实现。 (1)通过MHC I类基因分型详细描述猪尾猕猴的免疫遗传学特征。 (2)通过猪尾猕猴基因组单核苷酸多态性（SNP）遗传标记定位来表征猪尾猕猴的遗传学特征。 (3)确定MHC I类等位基因表达或SNP是否与猪尾猕猴中a）SIV相关疾病结局和B）自然发生的小肠结肠炎相关。

This resubmission of our animal resource grant renewal proposal requests five years of funding to continue support for a Specific Pathogen Free (SPF) colony of pig-tailed macaques (Macaca nemestrina) at the Johns Hopkins University that was first funded on September 21, 2006. It has the following resource component and research component aims: A. Resource Component Related Aims: (1) To expand the size of the current SPF breeding colony to increase the numbers of females for breeding and males available for sale to NIH funded investigators both within Johns Hopkins University and at other research institutions. (2) To maintain the SPF status of the colony by regular serologic and PCR testing for 4 viral agents: Cercopithecine herpesvirus 1 (B virus), Simian Immunodeficiency Virus (SIV), Simian Retrovirus (SRV) and Simian T-lymphotrophic Virus (STLV). At present all of the current members of the colony have repeatedly tested negative for these agents. (3) To maintain pedigree and health data on all animals. This data is available on all of the animals in the colony and will be expanded as the colony grows. B. Research Component Aims: To expand the genetic data available for this species to a) increase the research utility of the species and b) assist in disease management of the colony. This will be done through three research aims. (1) To characterize pig-tailed macaque immunogenetics in detail via MHC class I genotyping. (2) To characterize pig-tailed macaque genetics by single nucleotide polymorphism (SNP) genetic marker mapping throughout the pig-tailed macaque genome. (3) To determine whether either MHC class I allele expression or SNPs are associated with a) SIV-related disease outcomes and b) naturally occurring enterocolitis in pig-tailed macaques.