Primate Embryo Gene Expression Resource

该应用程序寻求对灵长类胚胎基因表达资源(PREGER)的持续支持。 PREGER的成立是为了解决有限的可获得性和获得非 人类灵长类(NHP)卵母细胞和胚胎用于研究目的，并作为改善人类 辅助生殖技术。卵子和胚胎可获得性的限制极大地限制了 NHP胚胎学的范围和速度，以及尝试进行NHP胚胎学研究的科学家的数量， 防止分子研究的增加与其他模型系统的增长同步进行。PREGER是一种 综合资源，采用成熟的、有良好文档记录的RT-PCR 从单个卵母细胞或胚胎中定量扩增整个信使核糖核酸的方法。 &gt；200包含一系列不同阶段和程序的cdna文库，可以扩增和 用于制备用于基因表达分析的斑点杂交。扩增的文库也可用于 基因发现即将到来。由于文库可以通过聚合酶链式反应重复扩增，样本 构成一种可持续的资源。这意味着为生产我们的产品而进行的初始投资 样本收集可以对基因表达进行快速、定量的分析，从而产生巨大的回报 而不需要获得额外的卵母细胞或胚胎。因此，节省的时间和成本都是 浩瀚无边。PREGER持续发展的三个目标是：(1)保持资源和 继续制作PREGER样本、斑点杂交、分子试剂和方法，进行基因表达 数据库，以及可供社区使用的基于Web的实用程序，作为NHP的首要分子资源 胚胎学，(2)使用DNA阵列极大地扩展我们资源的数据库部分，以推进我们的 NHP胚胎的知识，并提供独特的数据，以支持进一步发展安全和高效 艺术方法，(这延续了我们将小额和宝贵的初始投资转化为 将卵母细胞/胚胎转化为可广泛共享和分布的永久分子和数据资源 易于使用的表格)，以及(3)为青年科学家提供培训机会和支持，使他们能够 更容易进入NHP生殖生物学领域，并帮助他们解决日益紧迫的问题 需要对人类胚胎进行适当的NHP建模。实现这些目标将允许进一步 开发和应用PREGER资源促进NHP的发展和扩大 胚胎学研究，增加我们对人类和NHP胚胎的了解，并提供关键的 发展改进的临床艺术所需的信息基础。

This application seeks continued support for the Primate Embryo Gene Expression Resource (PREGER). PREGER was established to address constraints of limited availability and enormous costs of obtaining non- human primate (NHP) oocytes and embryos for research purposes, and as a model to improve human assisted reproduction technologies. Constraints of oocyte and embryo availability have greatly limited the scope and pace of NHP embryology, and the number of scientists attempting NHP embryology studies, preventing an increase in molecular studies in parallel with the growth of other model systems. PREGER is a comprehensive resource that was developed employing a well-established and well-documented RT-PCR method for quantitative amplification of entire mRNA populations from as little as a single oocyte or embryo. The >200 cDNA libraries, encompassing a diverse array of stages and procedures, can be amplified and used to prepare dot blots for gene expression analysis. The amplified libraries can also be employed for gene discovery approaches. Because the libraries can be repeatedly amplified by PCR, the samples constitute a sustainable resource. This means that the initial investment that has been made to produce our sample collection yields an immense pay-off by permitting rapid, quantitative analysis of gene expression without the need to obtain additional oocytes or embryos. The savings in both time and cost are therefore vast. The three objectives for the continued development of PREGER are: (1) Maintain the resource and continue to make PREGER samples, dot blots, molecular reagents and methods, the gene expression database, and the web-based utilities available to the community as the premier molecular resource for NHP embryology, (2) Vastly expand the database portion of our resource using DNA arrays to advance our knowledge of NHP embryos and provide unique data to support the further development of safe and efficient ART methods, (this continues our function of converting small-and precious-initial investments of oocytes/embryos into permanent molecular and data resources that can be widely shared and distributed in an easy-to-use form), and (3) Provide training opportunities and support to young scientists, to enable them to enter more readily the field of NHP reproductive biology, and help them to address the increasingly urgent need for appropriate NHP modeling of human embryos. Meeting these objectives will permit the further development and application of the PREGER resource to facilitate the growth and expansion of NHP embryology research, increase our understanding of human and NHP embryos, and provide the critical foundation of information needed to develop improved clinical ARTs.