XENOGRAFT STUDIES

异种移植骨芯的功能包括： 1.本核心的目的是为儿童实体肿瘤的异种移植提供一种设施， 在所有项目中使用。标准模型将包括在女性皮下空间生长的肿瘤 严重联合免疫缺陷(SCID)小鼠。 2.协调肿瘤移植，为荷瘤小鼠提供生化和 按要求进行药代动力学研究。 3.使用标准化方案进行药物评价研究。数据将被直接访问到 微型计算机，以及定期分发给个别项目负责人和 生物统计学核心分析。 4.建立微小残留病模型，用于评估细胞毒性和新的治疗方法 肿瘤根除(例如，基因治疗/反义)。 5.建立播散性疾病模型，作为药物评价的二级筛选。 6.用荧光素酶报告DNA损伤、缺氧等建立播散性疾病模型，进行监测 使用非侵入性技术(Xenogen系统)实现肿瘤生长、扩散和药物反应。 7.维持和鉴定人类异种移植物，并维持冻结的库存。 8.提供服务(例如，为小鼠采血、1至5天输液)。 9.根据需要评估转基因肿瘤模型。

The functions of the xenograft Core are: 1. The purpose of this Core is to provide a facility for the propagation of xenografts of pediatric solid tumors, for use in all Projects. The standard model will comprise tumor growing in the subcutaneous space of female severe combined immunodefiecient (SCID) mice. 2. Coordinate tumor transplantation and make available tumor-bearing mice for biochemical and pharmacokinetic studies as required. 3. Undertake drug evaluation studies using standardized protocols. Data will be accessed directly into a microcomputer, and information distributed on a regular basis to individual project leaders, and to the Biostatistics Core for analysis. 4. Establish models of minimal residual disease for evaluating both cytotoxic and novel approaches to tumor eradication (e.g. gene therapy/antisense). 5. Develop models of disseminated disease as a secondary screen for drug evaluation. 6. Develop disseminated disease models with luciferase reporters for DNA damage, hypoxia, to monitor tumor growth, spread and drug-response using non-invasive techniques (Xenogen system). 7. Maintain and characterize human xenografts,, and maintain frozen stocks. 8. Provide services (e.g. blood collection, 1 to 5 day infusions in mice). 9. To evaluate transgenic tumor models where requested.