CONTROL OF GONADOTROPIN SECRETION DURING LACTATION

这个子项目是利用资源的许多研究子项目之一 由NIH/NCRR资助的中心拨款提供。次级项目的主要支助 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 表示子项目使用的中心基础设施的估计数量， NCRR赠款不直接向子项目或子项目工作人员提供资金。 这一补充申请提供资金，使我们能够完成在2006年7月1日奖励开始时批准的母基金的具体目标1。 这个特定目标的目的是使用转基因GnRH-GFP大鼠和电生理技术记录GnRH细胞体，并确定与生殖和能量稳态调节相关的各种神经肽的直接作用。这一特定目标的假设是，食欲（NPY，食欲素，MCH）和食欲（NKB）神经肽是通过直接作用于GnRH神经元连接能量平衡和生殖功能的关键信号。这些补充资金使我们能够在我们的实验室中建立一个功能齐全的电生理学，并配备一名全职工作人员Verma博士，致力于这些研究。加上获资助的仪器项目，我们有能力进行这项拨款建议的所有研究。此外，随着松本博士作为电生理技术专家的共同研究者的招募，我们有专业知识来监督这项工作，并指导我们设计未来的实验。有了我们自己的设备和人员，我们将能够取得更大的进展，并探索其他因素，如kisspeptin，并研究突触前和突触后对GnRH活性的相互作用，如神经激肽B和kisspeptin，或NPY通过突触前Y1和突触后Y 5的作用。 这些研究的完成对于我们理解直接调节GnRH的关键神经肽至关重要。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. This supplemental request provides funding to enable us to complete Specific Aim 1 of the parent grant that was approved at the beginning of the award on July 1, 2006. The goal of this specific aim is to use transgenic GnRH-GFP rats and electrophysiological techniques to record from GnRH cell bodies and determine the direct effects of various neuropeptides related to regulation of reproduction and energy homeostasis. The hypothesis of this specific aim is that the orexigenic (NPY, orexin, MCH) and anorexigenic (NKB) neuropeptides are key signals linking energy balance and reproductive function through direct actions on GnRH neurons. These supplemental funds allow us to have a fully functional electrophysiology set up in our laboratory with a full time staff fellow, Dr. Verma, dedicated to these studies. With the addition of the funded equipment items, we are able to perform all the studies proposed in this grant. In addition, with the recruitment of Dr. Matsumoto as a Co-Investigator, an expert in electrophysiology techniques we have the expertise to supervise this work and guide us in the design of future experiments. With our own set up and personnel, we will be able to make much greater progress and to explore additional factors, such as kisspeptin, and to study interactions of presynaptic and postsynaptic effects on GnRH activity of factors such as neurokinin B and kisspeptin, or NPY's actions through presynaptic Y1 and postsynaptic Y5. Completion of these studies is critical to our understanding of the key neuropeptides that directly regulate GnRH.