INTRODUCING STABLE INFERTILITY BY RNA INTERFERENCE

通过 RNA 干扰引入稳定的不孕症

• 批准号: 8357818
• 负责人: Sergio R Ojeda
• 金额: $ 4.36万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8357818
• 关键词: Blood; Brain; Canis familiaris; Cells; Epitopes; Felis catus; Funding; Gene Expression; Goals; Grant; Home environment; Hypothalamic structure; Infertility; Iowa; National Center for Research Resources; Oregon; Ovary; Peptides; Phase; Population; Primates; Principal Investigator; RNA Interference; Rattus; Research; Research Infrastructure; Resources; Source; Stream; System; Tropism; United States National Institutes of Health; Universities; Viral; base; cost; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The overall goal of the present study is to develop a system for longlasting inhibition of gene expression in the brain or ovary that can be used to cause permanent infertility in dogs and cats. This application is a collaborative effort between the University of Iowa (UI) and the Oregon National Primate Research Center (ONPRC). We have performed several experiments aimed at achieving the objectives of the original application. These experiments involve the use of a bio-panning strategy to identify peptide epitopes that can be delivered via the blood stream and specifically home to cells in either the hypothalamus or the ovary of the rat to deliver RNAi. After five rounds of bio-panning, we selected an epitope that specifically home to the hypothalamus, ovary or both, and that can therefore be used for the next phase of the study: re-directing viral tropism of AAV based on these selected epitopes to deliver RNA interference to those particular cell populations.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 本研究的总体目标是开发一种长期抑制大脑或卵巢基因表达的系统，可用于导致狗和猫永久性不育。该应用程序是爱荷华大学 (UI) 和俄勒冈国家灵长类动物研究中心 (ONPRC) 之间的合作成果。 我们进行了几次实验，旨在实现最初应用的目标。这些实验涉及使用生物淘选策略来识别肽表位，这些肽表位可以通过血流递送，并特异性地归巢于大鼠下丘脑或卵巢中的细胞以递送RNAi。经过五轮生物淘选，我们选择了一个专门定位于下丘脑、卵巢或两者的表位，因此可用于下一阶段的研究：根据这些选定的表位重新引导 AAV 的病毒趋向性，以向那些特定的细胞群传递 RNA 干扰。