NIAMS Light Imaging Facility

在过去的一年里，来自13个部门、实验室或分支机构的研究人员使用了该设施，21名研究人员新接受了使用一种或几种仪器的培训。一如既往，LIS的工作人员帮助已经接受过培训的设备用户熟悉更苛刻的技术，如TIRF，以提高他们的图像质量，并为出版准备这些图像。在参考书目中列出了从这种帮助中受益和/或显示在我们的仪器上收集的图像的NIAMS出版物。 过去一年在LIS所做的显微镜研究有助于扩大NIAMS几个研究目标领域的知识： I)了解免疫系统及其疾病(Suzuki等人，2010年)； 二)了解皮肤发育和疾病(Duverger等人，2011年；Hwang等人，2011年)； 了解肌肉发育(Juan等人，2011年)和肌肉疾病(Raben等人，2010年a和b；Takikita等人，2010年；Xu等人，2010年)。 NIAMS研究人员可使用的显微镜技术范围已通过添加新的物镜Leica 25x 1.0而得到扩展，Leica 25x 1.0是一种高数值孔径、长工作距离的物镜，特别适用于活体显微镜。 在国会助手代表团的访问和整形外科医生学会的访问期间，该设施的工作人员演示了显微镜技术的使用及其在NIAMS目标区域的应用，在此期间，整形外科患者及其医生参观了该设施。

Researchers from 13 Sections, Laboratories, or Branches have used the Facility during the past year and 21 researchers have been newly trained to use one or several of the instruments. As always, LIS staff have assisted already trained Facility users to get familiar with the more demanding techniques such as TIRF, to improve the quality of their images, and to prepare these images for publication. NIAMS publications which have benefited from such help and/ or show images collected on our instruments are listed in the bibliography. Microscopy done in LIS in the past year has contributed to expand knowledge in several NIAMS research target areas: i) understanding the immune system and its diseases (Suzuki et al, 2010); ii) understanding skin development and diseases (Duverger et al., 2011; Hwang et al., 2011); iii) understanding muscle development (Juan et al., 2011) and muscle diseases (Raben et al., 2010 a and b; Takikita et al, 2010; Xu et al, 2010). The reach of the microscopy techniques available to NIAMS researchers has been expanded by the addition of a new objective lens, the Leica 25x 1.0, a high numerical aperture, long working distance objective that is particularly useful for intravital microscopy. Facility Staff have demonstrated the use of microscopy techniques and their applications to NIAMS target areas during a visit by a delegation of Congressional Aides and a visit by the Academy of Orthopedic Surgeons, during which orthopedic patients and their physicians toured the Facility.