NIAMS Light Imaging Facility

NIAMS 光成像设备

• 批准号: 10018444
• 负责人: Evelyn Ralston
• 金额: $ 82.29万
• 依托单位: NATIONAL INSTITUTE OF ARTHRITIS AND MUSCULOSKELETAL AND SKIN DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10018444
• 关键词: Arthritis; Autoimmunity; Blood; Computer Workstations; Computer software; Data; Equipment; Ethics; Human; Image; Image Analysis; Imaging Techniques; Immunology; Institutes; Journals; Knowledge; Laboratories; Lasers; Light; Lightning; Microscope; Microscopy; Mission; Molecular Genetics; Muscle; Myopathy; National Institute of Arthritis and Musculoskeletal and Skin Diseases; Publications; Publishing; Research Personnel; Resolution; Running; Scanning; Scientist; Slide; Techniques; Three-Dimensional Image; Tooth structure; Training; Work; base; confocal imaging; digital imaging; experience; flexibility; fluorescence microscope; fluorophore; imaging facilities; improved; instrument; interest; light microscopy; microscopic imaging; second harmonic generation imaging; skin disorder; two-photon

The past year has been very busy for the NIAMS Imaging Facility. Publications that include microscopy work carried out in the Light Imaging Section have been published in Blood, Immunology, Human Molecular Genetics and other high-quality scientific journals. These publications expand our knowledge of autoimmunity, muscle, and skin diseases. Researchers from 14 Sections, Laboratories, or Branches have used the Facility, 35 researchers have been trained to use one or more of the instruments and one presentation on the Bases of Microscopy & Ethical Imaging was offered to postdoctoral and postbac trainees. As every year, LIS staff have assisted Facility users to apply advanced microscopy techniques, to improve the quality of their images, to quantitate the images, and to prepare these images for publication. LIS staff have also evaluated new instruments that Facility users are interested in and worked on the replacement of out-of-date equipment. A Leica SP8 confocal microscope has replaced the Leica SP5 which dated from 2007. The SP8, besides producing high quality images is equipped with the Lightning software which offers super-resolution imaging. Its white light laser provides great flexibility in the choice of fluorophores to image. We have also recently replaced our workstation computer with a new, faster PC. The workstation drives our Incucytes and allows analysis of Incucyte data. It is also loaded with the LASX software that drives the Leica SP8 and allows analysis of the confocal images away from the instrument itself. It will also allow 3D image analysis with the Imaris software and post-acquisition deconvolution of confocal images. All our instruments have been in demand, including the Leica SP8 and the Zeiss 780 confocal microscopes, the Hamamatsu laser scanner, the Essen Zoom and S3 Incucytes, the Leica TIRF, and the Leica upright DM6000 and inverted DMI6000 fluorescence microscopes. The SP8 has been used in two-photon mode to collect Second Harmonic Generation images from muscle and teeth. There has been a sharp increase this year in the demand for slide scanning. More than 3,100 slides have been scanned in a year for scientists in 8 Laboratories/Branches working on arthritis, skin, and muscle diseases. The scanner is the only instrument which Light Imaging Section staff is running for the users who get direct access to their images stored on a shared server. All slides have been scanned by Ms. Kenea.

过去的一年是非常忙碌的NIAMS成像设施。包括光学成像科显微镜工作在内的出版物已在《血液》、《免疫学》、《人类分子遗传学》和其他高质量科学期刊上发表。这些出版物扩展了我们对自身免疫、肌肉和皮肤疾病的认识。 来自14个部门，实验室或分支机构的研究人员使用了该设施，35名研究人员接受了使用一种或多种仪器的培训，并向博士后和postbac学员提供了一次关于显微镜和伦理成像基础的演示。 与每年一样，地雷影响调查工作人员协助设施用户应用先进的显微技术，提高图像质量，对图像进行定量，并准备这些图像供出版。地雷影响调查工作人员还对设施用户感兴趣的新仪器进行了评估，并努力更换过时的设备。Leica SP 8共聚焦显微镜取代了2007年的Leica SP 5。SP 8除了生成高质量图像外，还配备了Lightning软件，可提供超分辨率成像。其白色激光器在选择荧光团成像方面提供了极大的灵活性。我们最近还将工作站计算机更换为速度更快的新PC。工作站驱动我们的Incucyte并允许分析Incucyte数据。它还装载了LASX软件，该软件驱动Leica SP 8，并允许在远离仪器本身的情况下分析共焦图像。它还将允许使用Imaris软件进行3D图像分析和共焦图像的采集后去卷积。我们所有的仪器都有需求，包括Leica SP 8和Zeiss 780共聚焦显微镜、Hamamatsu激光扫描仪、埃森Zoom和S3 Incucytes、Leica TIRF以及Leica直立DM 6000和倒置DMI 6000荧光显微镜。SP 8已在双光子模式下用于采集肌肉和牙齿的二次谐波生成图像。今年对幻灯片扫描的需求急剧增加。 在一年内为8个实验室/分支机构的科学家扫描了3，100多张载玻片，这些科学家致力于关节炎，皮肤和肌肉疾病。扫描仪是光成像科工作人员为用户运行的唯一仪器，用户可以直接使用储存在共享服务器上的图像。所有切片均由Kenea女士扫描。