NIAMS Light Imaging Facility

在过去一年中，来自13个科、实验室或分支机构的研究人员使用了该设施，25名研究人员接受了使用一种或多种仪器的新培训。 与每年一样，地雷影响调查工作人员协助设施用户应用先进的显微技术，提高图像质量，对图像进行定量，并准备这些图像供出版。参考书目中列出了从此类帮助中受益的NIAMS出版物和/或显示我们仪器上收集的图像。地雷影响调查工作人员还帮助评价设施用户感兴趣的新仪器。 LIS工作人员开发了新的检测和软件来评估和定量核转位，并评估和定量趋化性。这些项目是应几个处或实验室用户的要求而开展的。 在NIAMS研究人员的出版物中，如果没有在光成像部分进行的显微镜工作，就不可能完成，这些出版物扩展了我们对几个NIAMS研究目标领域的知识，如关节炎（PaiK等人，出版中）;皮肤和牙齿发育和疾病（Lessard等人; Kim等人）;和肌肉发育（Ryall et al. 2015）。 该基金的每一项工具都得到了很好的利用。Leica Slidescanner 400 SCN可收集带有组织学样本或荧光标记组织切片的整个载玻片的扫描结果，已得到稳定使用。存储内存已经增加，以满足扫描数量快速增长所产生的需求。对于组织学样本，载玻片扫描仪以自动模式工作。它产生高质量的图像，可以通过大范围的放大在线可视化，“一个谷歌地图。“该仪器由LIS工作人员专门处理。已为光成像科以外的9个实验室/分支机构的用户扫描了2，000多张幻灯片。偶尔我们也会帮助其他IRP的同事扫描他们的幻灯片。

Researchers from 13 Sections, Laboratories, or Branches have used the Facility during the past year and 25 researchers have been newly trained to use one or more of the instruments. As every year, LIS staff have assisted Facility users to apply advanced microscopy techniques, to improve the quality of their images, to quantitate the images, and to prepare these images for publication. NIAMS publications which have benefited from such help and/or show images collected on our instruments are listed in the bibliography. LIS staff have also helped with evaluation of new instruments that Facility users are interested in. LIS Staff has developed new assays and software to evaluate and quantitate nuclear translocation, and to evaluate and quantitate chemotaxis. These projects were carried out in response to demand by users from several Branches or Laboratories. Among NIAMS investigators' publications which could not have been completed without microscopy work carried out in the Light Imaging Section, were publications expanding our knowledge of several NIAMS research target areas such as arthritis (PaiK et al. In Press); skin and tooth development and diseases (Lessard et al; Kim et al.); and muscle development (Ryall et al. 2015). Each instrument of the Facility has been put to good use. The Leica Slidescanner 400SCN that collects scans of whole slides with histology samples or fluorescently labeled tissue slices has been used steadily. Storage memory has been increased to address the needs created by a fast growth in scan numbers. For histology samples the Slidescanner works in automated mode. It produces high quality images that can be visualized online through a large range of magnifications, "a la Google map." The instrument is handled by LIS staff exclusively. More than 2,000 slides have been scanned for users in 9 Laboratories/Branches outside of the Light Imaging Section. Occasionally we have helped colleagues in other IRPs by scanning their slides.