MATERNAL OBESITY IN PREGNANCY FETAL, PLACENTAL AND MATERNAL EFFECTS

孕期母亲肥胖对胎儿、胎盘和母体的影响

• 批准号: 8357676
• 负责人: PETER W. NATHANIELSZ
• 金额: $ 2.02万
• 依托单位: TEXAS BIOMEDICAL RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8357676
• 关键词: Abdomen; Adrenal Glands; Animal Feed; Animals; Autopsy; Biopsy; Blood Vessels; Brain Stem; Breeding; Data; Diet; Fatty acid glycerol esters; Fetus; Flavoring; Funding; Gastrocnemius Muscle; Glycogen; Goals; Grant; Hypothalamic structure; Liver; Lymphocyte; Metabolic; Mothers; Muscle; National Center for Research Resources; Obesity; Omentum; Organ; Pancreas; Papio; Physiology; Population; Pregnancy; Primates; Principal Investigator; Research; Research Infrastructure; Resources; Sampling; Soleus Muscle; Source; Tissues; United States National Institutes of Health; cohort; cost; feeding; fetal; fetal blood; insulin signaling; subcutaneous

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. We have made a cohort of baboons that is significantly heavier (p 0.05) in comparison to control-fed animals by feeding them ad lib with the high fat, highly palatable SFBR "pink" diet with nocaloric lemonade flavoring added. Having accomplished this goal, we are now requesting to breed 7 control and 8 treated animals to get data on control and overfed mothers and their fetuses with respect to a number of metabolic endpoints including maternal and fetal fat composition, fetal blood vessel physiology, insulin signaling in fat and muscle, glycogen storage and lymphocyte populations. At c-section at 90 ¿ 5 days of gestation (dG; range), samples of maternal femoral and abdominal subcutaneous fat and omental and perirenal fat and biopsies of soleus and gastrocnemius muscle will be taken. Finally, we will necropsy the fetuses to look at a number of tissues/organs including, but not limited to, pancreas, liver, fat, muscle, adrenal glands, microvasculature and feeding control centers in the hypothalamus and brainstem.

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 我们用添加了无热量柠檬水调味剂的高脂肪、非常可口的SFBR“粉色”食物随意喂食一群与对照组动物相比体重明显增加(p<0.05)的狒狒。完成这一目标后，我们现在要求培育7只对照动物和8只处理动物，以获得关于母体和胎儿脂肪成分、胎儿血管生理、脂肪和肌肉中的胰岛素信号、糖原储存和淋巴细胞数量等代谢终点的控制和过度喂养母亲及其胎儿的数据。在妊娠90~5天(DG；范围)剖腹产时，取母体股骨和腹部皮下脂肪、大网膜和肾周脂肪以及比目鱼肌和腓肠肌的活检组织。最后，我们将对胎儿进行尸检，以查看一些组织/器官，包括但不限于胰腺、肝脏、脂肪、肌肉、肾上腺、微血管以及下丘脑和脑干的摄食控制中心。