MATERNAL OBESITY IN PREGNANCY FETAL, PLACENTAL AND MATERNAL EFFECTS

孕期母亲肥胖对胎儿、胎盘和母体的影响

• 批准号: 8357676
• 负责人: PETER W. NATHANIELSZ
• 金额: $ 2.02万
• 依托单位: TEXAS BIOMEDICAL RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8357676
• 关键词: Abdomen; Adrenal Glands; Animal Feed; Animals; Autopsy; Biopsy; Blood Vessels; Brain Stem; Breeding; Data; Diet; Fatty acid glycerol esters; Fetus; Flavoring; Funding; Gastrocnemius Muscle; Glycogen; Goals; Grant; Hypothalamic structure; Liver; Lymphocyte; Metabolic; Mothers; Muscle; National Center for Research Resources; Obesity; Omentum; Organ; Pancreas; Papio; Physiology; Population; Pregnancy; Primates; Principal Investigator; Research; Research Infrastructure; Resources; Sampling; Soleus Muscle; Source; Tissues; United States National Institutes of Health; cohort; cost; feeding; fetal; fetal blood; insulin signaling; subcutaneous

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. We have made a cohort of baboons that is significantly heavier (p 0.05) in comparison to control-fed animals by feeding them ad lib with the high fat, highly palatable SFBR "pink" diet with nocaloric lemonade flavoring added. Having accomplished this goal, we are now requesting to breed 7 control and 8 treated animals to get data on control and overfed mothers and their fetuses with respect to a number of metabolic endpoints including maternal and fetal fat composition, fetal blood vessel physiology, insulin signaling in fat and muscle, glycogen storage and lymphocyte populations. At c-section at 90 ¿ 5 days of gestation (dG; range), samples of maternal femoral and abdominal subcutaneous fat and omental and perirenal fat and biopsies of soleus and gastrocnemius muscle will be taken. Finally, we will necropsy the fetuses to look at a number of tissues/organs including, but not limited to, pancreas, liver, fat, muscle, adrenal glands, microvasculature and feeding control centers in the hypothalamus and brainstem.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 与对照喂养的动物相比，我们制作了一系列狒狒，与对照喂养的动物相比，它们与脂肪高，高度可口的SFBR“粉红色”饮食相比，并加入了nocaloric柠檬水调味料。实现了这一目标后，我们现在要求繁殖7种控制和8种治疗的动物，以获取有关许多代谢终点的控制和过度喂食母亲及其胎儿的数据，包括母子和胎儿脂肪组成，胎儿血管生理学，脂肪和肌肉中的胰岛素信号，糖原储存和淋巴细胞群中的胰岛素信号。在90»妊娠5天（DG;范围）的剖腹产时，将采集股骨和腹部皮下脂肪和腹膜下脂肪，腹膜脂肪以及甲氧肌和胃肌肉肌肉的活检。最后，我们将胎儿尸检查看许多组织/器官，包括但不限于胰腺，肝脏，脂肪，肌肉，肌肉，肾上腺，微脉管系统，微脉管和喂养控制中心，以及下丘脑和脑干的喂养控制中心。