MATERNAL OBESITY IN PREGNANCY FETAL, PLACENTAL AND MATERNAL EFFECTS

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 我们通过随意饲喂添加无热量柠檬水调味剂的高脂肪、高适口性SFBR“粉红色”饲料，制备了一组狒狒，与对照组动物相比，其体重显著增加（p 0.05）。 在实现这一目标后，我们现在要求对7只对照组和8只给药组动物进行繁殖，以获得对照组和过度喂养组母体及其胎仔在许多代谢终点方面的数据，包括母体和胎仔脂肪组成、胎仔血管生理学、脂肪和肌肉中的胰岛素信号传导、糖原储存和淋巴细胞群。 在妊娠90 - 5天（dG;范围）剖腹产时，采集母体股骨和腹部皮下脂肪、网膜和肾周脂肪样本以及比目鱼肌和腓肠肌活检样本。 最后，我们将对胎仔进行尸检，以观察许多组织/器官，包括但不限于胰腺、肝脏、脂肪、肌肉、肾上腺、微血管系统以及下丘脑和脑干中的摄食控制中心。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. We have made a cohort of baboons that is significantly heavier (p 0.05) in comparison to control-fed animals by feeding them ad lib with the high fat, highly palatable SFBR "pink" diet with nocaloric lemonade flavoring added. Having accomplished this goal, we are now requesting to breed 7 control and 8 treated animals to get data on control and overfed mothers and their fetuses with respect to a number of metabolic endpoints including maternal and fetal fat composition, fetal blood vessel physiology, insulin signaling in fat and muscle, glycogen storage and lymphocyte populations. At c-section at 90 ¿ 5 days of gestation (dG; range), samples of maternal femoral and abdominal subcutaneous fat and omental and perirenal fat and biopsies of soleus and gastrocnemius muscle will be taken. Finally, we will necropsy the fetuses to look at a number of tissues/organs including, but not limited to, pancreas, liver, fat, muscle, adrenal glands, microvasculature and feeding control centers in the hypothalamus and brainstem.