COBRE: UND: AUGMENTATION OF IMAGING CORE FACILITY

COBRE：UND：成像核心设施的增强

• 批准号: 8360137
• 负责人: BRYON D GROVE
• 金额: $ 12.7万
• 依托单位: UNIVERSITY OF NORTH DAKOTA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-07-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8360137
• 关键词: Centers of Research Excellence; Computer Workstations; Computer software; Core Facility; Data; Dyes; Electron Microscopy Facility; Fluorescence; Fluorescence Microscopy; Funding; Grant; Health Sciences; Image; Image Analysis; Immunofluorescence Microscopy; Label; Lasers; Microscope; Modeling; National Center for Research Resources; Neurodegenerative Disorders; North Dakota; Optics; Principal Investigator; Process; Research; Research Infrastructure; Research Personnel; Resources; Scanning; Signal Transduction; Source; Spectrum Analysis; Spottings; System; United States National Institutes of Health; Universities; cost; design; digital; fluorophore; medical schools; meetings

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The objectives here are to augment existing imaging core facilities in the School of Medicine and Health Sciences and to enhance imaging capacity and capabilities at the University of North Dakota. The existing imaging core is equipped with an Hitachi TEM, an Hitachi SEM, an Olympus Fluoview 300 laser scanning confocal microscope, and two older model epifluorescence microscopes, one of which is equipped with a Spot II digital camera. While the electron microscopy facilities are state-of-the-art, the confocal and the fluorescence microscopy facilities had several limitations. First, the confocal microscope is equipped with only two channels and possesses a laser configuration which does not permit the use of dyes excited by near UV. The system is not upgradable and therefore investigators are effectively limited to dual label imaging with a restricted list of fluorophores. Second, the existing epifluorescence microscopes are inadequate for most immunofluorescence microscopy applications due to their older optical design. To overcome these limitations and to meet the needs of Projects 1, 3, and 5, the imaging core was augmented by the addition of 1) a Zeiss 510 Meta confocal microscope capable of eight channel imaging (seven fluorescent channels/one DIC channel) and detecting a wide range of fluorophores, 2) a confoCor2 FCS unit for fluorescence correlation spectroscopy, 3) an Axiovert200 microscope with a sensitive, fast digital camera (AxioCam HRM), and 4) a computer workstation with Zeiss software for processing and analyzing image data. These resources were required for three of the projects in the COBRE application. Their addition greatly enhances the research capabilities of investigators in the School of Medicine and the University of North Dakota.

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 这里的目标是加强医学和健康科学学院现有的成像核心设施，并加强北达科他州大学的成像能力和能力。现有的成像核心配备了一台日立电子显微镜、一台日立扫描电子显微镜、一台奥林巴斯FluoView 300激光扫描共焦显微镜，以及两台较老型号的荧光显微镜，其中一台配备了SPOT II数码相机。虽然电子显微镜设备是最先进的，但共焦显微镜和荧光显微镜设备有几个局限性。首先，共焦显微镜只配备两个通道，并具有激光配置，不允许使用由近紫外线激发的染料。该系统是不可升级的，因此研究人员实际上被限制在双标记成像和有限的荧光团名单上。其次，现有的荧光显微镜由于其较旧的光学设计而不能满足大多数免疫荧光显微镜的应用。为了克服这些限制并满足项目1、3和5的需求，成像核心得到了扩展，增加了1)一台蔡司510Meta共焦显微镜，它能够进行八通道成像(七个荧光通道/一个DIC通道)并检测各种荧光团；2)一台用于荧光相关光谱的ConfoCor2 FCS单元；3)一台配备灵敏快速数码相机(AxioCam HRM)的Axiovert200显微镜；以及4)一台安装了蔡司软件的计算机工作站，用于处理和分析图像数据。Cobre应用程序中的三个项目需要这些资源。他们的加入极大地增强了医学院和北达科他州大学研究人员的研究能力。