COBRE: UND: AUGMENTATION OF IMAGING CORE FACILITY

COBRE：UND：成像核心设施的增强

• 批准号: 8360137
• 负责人: BRYON D GROVE
• 金额: $ 12.7万
• 依托单位: UNIVERSITY OF NORTH DAKOTA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-07-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8360137
• 关键词: Centers of Research Excellence; Computer Workstations; Computer software; Core Facility; Data; Dyes; Electron Microscopy Facility; Fluorescence; Fluorescence Microscopy; Funding; Grant; Health Sciences; Image; Image Analysis; Immunofluorescence Microscopy; Label; Lasers; Microscope; Modeling; National Center for Research Resources; Neurodegenerative Disorders; North Dakota; Optics; Principal Investigator; Process; Research; Research Infrastructure; Research Personnel; Resources; Scanning; Signal Transduction; Source; Spectrum Analysis; Spottings; System; United States National Institutes of Health; Universities; cost; design; digital; fluorophore; medical schools; meetings

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The objectives here are to augment existing imaging core facilities in the School of Medicine and Health Sciences and to enhance imaging capacity and capabilities at the University of North Dakota. The existing imaging core is equipped with an Hitachi TEM, an Hitachi SEM, an Olympus Fluoview 300 laser scanning confocal microscope, and two older model epifluorescence microscopes, one of which is equipped with a Spot II digital camera. While the electron microscopy facilities are state-of-the-art, the confocal and the fluorescence microscopy facilities had several limitations. First, the confocal microscope is equipped with only two channels and possesses a laser configuration which does not permit the use of dyes excited by near UV. The system is not upgradable and therefore investigators are effectively limited to dual label imaging with a restricted list of fluorophores. Second, the existing epifluorescence microscopes are inadequate for most immunofluorescence microscopy applications due to their older optical design. To overcome these limitations and to meet the needs of Projects 1, 3, and 5, the imaging core was augmented by the addition of 1) a Zeiss 510 Meta confocal microscope capable of eight channel imaging (seven fluorescent channels/one DIC channel) and detecting a wide range of fluorophores, 2) a confoCor2 FCS unit for fluorescence correlation spectroscopy, 3) an Axiovert200 microscope with a sensitive, fast digital camera (AxioCam HRM), and 4) a computer workstation with Zeiss software for processing and analyzing image data. These resources were required for three of the projects in the COBRE application. Their addition greatly enhances the research capabilities of investigators in the School of Medicine and the University of North Dakota.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 这里的目标是增加现有的医学和健康科学学院的成像核心设施，并提高北达科他州大学的成像能力和能力。现有的成像核心配备有Hitachi TEM、Hitachi SEM、Olympus Fluoview 300激光扫描共聚焦显微镜和两个较旧型号的落射荧光显微镜，其中一个配备有Spot II数码相机。虽然电子显微镜设施是最先进的，但共聚焦和荧光显微镜设施有一些局限性。首先，共聚焦显微镜仅配备有两个通道，并且具有不允许使用由近UV激发的染料的激光配置。该系统不可复制，因此研究者实际上仅限于使用有限的荧光团列表进行双标记成像。其次，现有的落射荧光显微镜是不够的，大多数免疫荧光显微镜应用，由于其较旧的光学设计。为了克服这些限制并满足项目1、3和5的需要，通过增加1）能够进行八通道成像的Zeiss 510 Meta共聚焦显微镜来增强成像核心（七个荧光通道/一个DIC通道）并检测宽范围的荧光团，2）用于荧光相关光谱的confoCor 2 FCS单元，3）具有灵敏度的Axiovert 200显微镜，快速数码相机（AxioCam HRM），和4）具有Zeiss软件的计算机工作站，用于处理和分析图像数据。COBRE应用程序中的三个项目需要这些资源。他们的加入大大增强了医学院和北达科他州大学研究人员的研究能力。