Imaging Core

摘要该项目的目标是支持COBRE的成像和图像分析需求 第二阶段计划，同时继续支持更广泛的研究社区。所有四位第2阶段项目负责人 建议确定和描述控制宿主对病毒、细菌和寄生虫反应的因素 病原体使用共聚焦，荧光和电子显微镜和定量图像分析。核心是 很好地满足了这些需求。核心设备包括用于活体显微镜检查的多光子显微镜， 共聚焦显微镜，两个落射荧光显微镜系统，一个能够TIRF显微镜和两个 配备活细胞成像，扫描和透射电子显微镜，以及一个工作站， 图像分析工具。该核心提出了四个具体目标，以确保COBRE调查人员（第二阶段和第三阶段） 第1阶段项目负责人）和更广泛的研究社区可以实现他们的研究目标。第一，核心 主任和工作人员将与COBRE研究人员和研究团体的其他成员合作， 协助实验设计和显微镜方法的发展，分析显微镜 图像，并解释核心中收集的数据。第二，核心人员将提供设备使用培训， 显微成像服务，以及COBRE调查人员和其他成员的教育资源， 研究社区。第三，核心将提供图像分析软件工具和专业知识支持 COBRE研究人员和研究界其他成员的定量显微镜研究。最后， 核心将通过更换过时的落射荧光显微镜和寻求资金来升级其设备 通过S10共享仪器资助计划，以取代核心蔡司共聚焦显微镜， 新的共聚焦显微镜系统。这些目标的结果将是扩大核心服务， 核心研究能力，为用户提供新的研究机会，并推进核心的目标扩展 用户群，实现长期可持续发展。

SUMMARY. The objective of this project is to support the imaging and image analysis needs of the COBRE Phase 2 program while continuing to support the broader research community. All four Phase 2 Project Leaders propose to identify and characterize the factors that control host responses to viral, bacterial, and parasitic pathogens using confocal, fluorescence and electron microscopy and quantitative image analysis. The Core is well equipped to meet these needs. Core equipment includes a multiphoton microscope for intravital microscopy, a confocal microscope, two epifluorescence microscope systems, one capable of TIRF microscopy and both equipped for live cell imaging, scanning and transmission electron microscopes, and a workstation with several image analysis tools. The Core proposes four specific aims to ensure that COBRE investigators (Phase 2 and Phase 1 Project Leaders) and the broader research community can meet their research goals. First, the Core director and staff will collaborate with COBRE investigators and other members of the research community by assisting with the development of experimental designs and microscopy approaches, analyzing microscopy images, and interpreting data collected in the Core. Second, Core staff will provide training in equipment use, microscopic imaging services, and educational resources to COBRE investigators and other members of the research community. Third, the Core will provide image analysis software tools and expertise to support quantitative microscopy studies by COBRE investigators and other members of the research community. Finally, the Core will upgrade its equipment by replacing an outdated epifluorescence microscope and seeking funding through the S10 Shared Instrumentation Grant Program to replace the Core Zeiss confocal microscope with a new confocal microscope system. The outcome of these aims will be expansion of Core services, increased Core research capabilities, new research opportunities for users and advancement of the Core’s goals to expand the user base and achieve long-term sustainability.