Imaging Core

总结。该项目的目标是支持眼镜蛇的成像和图像分析需求 第二阶段计划，同时继续支持更广泛的研究社区。所有四位第二阶段项目负责人 建议确定和表征控制宿主对病毒、细菌和寄生虫的反应的因素 利用共聚焦、荧光和电子显微镜以及定量图像分析检测病原体。核心是 装备精良，足以满足这些需求。核心设备包括用于活体显微镜的多光子显微镜， 一个共聚焦显微镜，两个荧光显微镜系统，一个能进行TIRF显微镜，两个都能 配备活细胞成像、扫描和透射电子显微镜，以及具有多个 图像分析工具。核心提出了四个具体目标，以确保Cobre调查人员(第二阶段和 第一阶段项目负责人)和更广泛的研究社区可以实现他们的研究目标。第一，核心 主任和工作人员将与Cobre调查人员和研究社区的其他成员合作 协助开发实验设计和显微镜方法，分析显微镜 图像，并解释在核心收集的数据。第二，核心员工将提供设备使用方面的培训， 为Cobre调查人员和其他成员提供显微成像服务和教育资源 研究社区。第三，核心将提供图像分析软件工具和专业知识，以支持 Cobre研究人员和研究界其他成员进行的定量显微镜研究。最后， Core将通过更换过时的荧光显微镜并寻求资金来升级其设备 通过S10共享仪器资助计划将核心蔡司共焦显微镜更换为 新的共焦显微镜系统。这些目标的结果将是核心服务的扩展，增加 核心研究能力、用户的新研究机会以及核心扩展目标的推进 并实现用户基础和长期可持续性。

SUMMARY. The objective of this project is to support the imaging and image analysis needs of the COBRE Phase 2 program while continuing to support the broader research community. All four Phase 2 Project Leaders propose to identify and characterize the factors that control host responses to viral, bacterial, and parasitic pathogens using confocal, fluorescence and electron microscopy and quantitative image analysis. The Core is well equipped to meet these needs. Core equipment includes a multiphoton microscope for intravital microscopy, a confocal microscope, two epifluorescence microscope systems, one capable of TIRF microscopy and both equipped for live cell imaging, scanning and transmission electron microscopes, and a workstation with several image analysis tools. The Core proposes four specific aims to ensure that COBRE investigators (Phase 2 and Phase 1 Project Leaders) and the broader research community can meet their research goals. First, the Core director and staff will collaborate with COBRE investigators and other members of the research community by assisting with the development of experimental designs and microscopy approaches, analyzing microscopy images, and interpreting data collected in the Core. Second, Core staff will provide training in equipment use, microscopic imaging services, and educational resources to COBRE investigators and other members of the research community. Third, the Core will provide image analysis software tools and expertise to support quantitative microscopy studies by COBRE investigators and other members of the research community. Finally, the Core will upgrade its equipment by replacing an outdated epifluorescence microscope and seeking funding through the S10 Shared Instrumentation Grant Program to replace the Core Zeiss confocal microscope with a new confocal microscope system. The outcome of these aims will be expansion of Core services, increased Core research capabilities, new research opportunities for users and advancement of the Core’s goals to expand the user base and achieve long-term sustainability.