Imaging Core

成像核心

基本信息

批准号：
10270974
负责人：
BRYON D GROVE
金额：
$ 21.28万
依托单位：
UNIVERSITY OF NORTH DAKOTA
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-05-13 至 2026-06-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10270974
关键词：
3-Dimensional Address Aging Alzheimer&apos s Disease Biomedical Research Cells Centers of Research Excellence Communities Computer Workstations Confocal Microscopy Core Facility Coupled Data Development Drug Addiction Education Educational workshop Electron Microscope Electron Microscopy Ensure Equipment Experimental Designs Fees Fiji Fluorescence Microscopy Funding Goals Grant Health Sciences Image Image Analysis Immune response Lyme Disease Malignant Neoplasms Mental Depression Microscope Microscopy Mission Modeling Names Outcome Phase Physiological Quantitative Microscopy Records Research Research Activity Research Personnel Research Training Scanning Services Software Tools Specialist System Three-dimensional analysis Total Internal Reflection Fluorescent Training Viral base design education resources experience experimental study imaging approach imaging capabilities instrument instrumentation interest intravital microscope intravital microscopy live cell imaging machine learning algorithm medical schools member microscopic imaging operation pathogen programs quantitative imaging tool transmission process web site webinar

项目摘要

SUMMARY. The objective of this project is to support the imaging and image analysis needs of the COBRE Phase 2 program while continuing to support the broader research community. All four Phase 2 Project Leaders propose to identify and characterize the factors that control host responses to viral, bacterial, and parasitic pathogens using confocal, fluorescence and electron microscopy and quantitative image analysis. The Core is well equipped to meet these needs. Core equipment includes a multiphoton microscope for intravital microscopy, a confocal microscope, two epifluorescence microscope systems, one capable of TIRF microscopy and both equipped for live cell imaging, scanning and transmission electron microscopes, and a workstation with several image analysis tools. The Core proposes four specific aims to ensure that COBRE investigators (Phase 2 and Phase 1 Project Leaders) and the broader research community can meet their research goals. First, the Core director and staff will collaborate with COBRE investigators and other members of the research community by assisting with the development of experimental designs and microscopy approaches, analyzing microscopy images, and interpreting data collected in the Core. Second, Core staff will provide training in equipment use, microscopic imaging services, and educational resources to COBRE investigators and other members of the research community. Third, the Core will provide image analysis software tools and expertise to support quantitative microscopy studies by COBRE investigators and other members of the research community. Finally, the Core will upgrade its equipment by replacing an outdated epifluorescence microscope and seeking funding through the S10 Shared Instrumentation Grant Program to replace the Core Zeiss confocal microscope with a new confocal microscope system. The outcome of these aims will be expansion of Core services, increased Core research capabilities, new research opportunities for users and advancement of the Core’s goals to expand the user base and achieve long-term sustainability.

概括。该项目的目的是支持毛绒的成像和图像分析需求第二阶段计划在继续支持更广泛的研究社区的同时。所有四个阶段2项目负责人识别和表征控制宿主对病毒，细菌和寄生虫的反应的因素的建议使用共聚焦，荧光和电子显微镜以及定量图像分析的病原体。核心是能够满足这些需求的设备齐全。核心设备包括用于插入显微镜的多光子显微镜，共聚焦显微镜，两个落荧光显微镜系统，一个能够进行TIRF显微镜，两者都配备了活细胞成像，扫描和传输电子显微镜，以及一个工作站图像分析工具。核心提案的四个具体旨在确保毛病调查员（2阶段和阶段第1阶段项目负责人）和更广泛的研究社区可以实现其研究目标。首先，核心董事和工作人员将与COBRE调查人员和研究社区的其他成员合作协助开发实验设计和显微镜方法，分析显微镜图像，并解释核心收集的数据。第二，核心人员将提供设备使用的培训，微观成像服务以及毛的调查人员和其他成员的教育资源研究社区。第三，核心将提供图像分析软件工具和专业知识来支持毛cor研究者和研究界其他成员的定量显微镜研究。最后，核心将通过更换过时的落荧光显微镜并寻求资金来升级其设备通过S10共享的仪器赠款计划，用A代替核心Zeiss共焦显微镜新的共聚焦显微镜系统。这些目标的结果将是扩大核心服务，增加核心研究能力，用户的新研究机会以及核心扩展目标的发展用户群并实现长期可持续性。