COBRE: UND: AUGMENTATION OF IMAGING CORE FACILITY

COBRE：UND：成像核心设施的增强

• 批准号: 7381905
• 负责人: BRYON D GROVE
• 金额: $ 16.22万
• 依托单位: UNIVERSITY OF NORTH DAKOTA
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-01 至 2007-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7381905
• 关键词: COBRE; UND; AUGMENTATION; IMAGING; CORE

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The objectives of this project are to augment the existing imaging core facilities in the Department of Anatomy and Cell Biology and to enhance the imaging resources available to investigators at the University of North Dakota. The existing imaging core is equipped with an Hitachi TEM, an Hitachi SEM, an Olympus Fluoview 300 laser scanning confocal microscope, and two older model epifluorescence microscopes, one of which is equipped with a Spot II digital camera. While the electron microscopy facilities are state-of-the-art, the confocal and the fluorescence microscopy facilities had several limitations. First, the confocal microscope is equipped with only two channels and possesses a laser configuration which does not permit the use of dyes excited by near UV. The system is not upgradable and therefore investigators are effectively limited to dual label imaging with a restricted list of fluorophores. Second, the existing epifluorescence microscopes are inadequate for most immunofluorescence microscopy applications due to their older optical design. To overcome these limitations and to meet the needs of Projects 1, 3, and 5, the imaging core was augmented by the addition of 1) a Zeiss 510 Meta confocal microscope capable of eight channel imaging (seven fluorescent channels/one DIC channel) and detecting a wide range of fluorophores, 2) a confoCor2 FCS unit for fluorescence correlation spectroscopy, 3) an Axiovert200 microscope with a sensitive, fast digital camera (AxioCam HRM), and 4) a computer workstation with Zeiss software for processing and analyzing image data. These resources were required for three of the projects in the COBRE application. Their addition greatly enhances the research capabilities of investigators in the School of Medicine at the University of North Dakota.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。所列机构为中心机构，不一定为研究者机构。该项目的目标是增加解剖学和细胞生物学系现有的成像核心设施，并提高北达科他州大学研究人员可用的成像资源。现有的成像核心配备有Hitachi TEM、Hitachi SEM、Olympus Fluoview 300激光扫描共聚焦显微镜和两个较旧型号的落射荧光显微镜，其中一个配备有Spot II数码相机。虽然电子显微镜设备是最先进的，但共聚焦和荧光显微镜设备有几个限制。首先，共聚焦显微镜仅配备有两个通道，并且具有不允许使用由近UV激发的染料的激光配置。该系统不可复制，因此研究者实际上仅限于使用有限的荧光团列表进行双标记成像。其次，现有的落射荧光显微镜是不够的，大多数免疫荧光显微镜应用，由于其较旧的光学设计。为了克服这些限制并满足项目1、3和5的需要，通过增加1）能够进行八通道成像的Zeiss 510 Meta共聚焦显微镜来增强成像核心（七个荧光通道/一个DIC通道）并检测宽范围的荧光团，2）用于荧光相关光谱的confoCor 2 FCS单元，3）具有灵敏度的Axiovert 200显微镜，快速数码相机（AxioCam HRM），和4）具有Zeiss软件的计算机工作站，用于处理和分析图像数据。COBRE应用程序中的三个项目需要这些资源。他们的加入大大增强了北达科他州大学医学院研究人员的研究能力。