COBRE: UND: AUGMENTATION OF IMAGING CORE FACILITY

COBRE：UND：成像核心设施的增强

• 批准号: 7610481
• 负责人: BRYON D GROVE
• 金额: $ 16.73万
• 依托单位: UNIVERSITY OF NORTH DAKOTA
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-08-07 至 2008-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7610481
• 关键词: Arts; Centers of Research Excellence; Computer Retrieval of Information on Scientific Projects Database; Computer Workstations; Computer software; Core Facility; Data; Dyes; Electron Microscopy Facility; Fluorescence; Fluorescence Microscopy; Funding; Grant; Health Sciences; Image; Image Analysis; Immunofluorescence Microscopy; Institution; Label; Lasers; Microscope; Modeling; North Dakota; Optics; Process; Range; Research; Research Personnel; Resources; Scanning; Source; Spectrum Analysis; Spottings; System; United States National Institutes of Health; Universities; design; digital; fluorophore; medical schools

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The objectives here are to augment existing imaging core facilities in the School of Medicine and Health Sciences and to enhance imaging capacity and capabilities at the University of North Dakota. The existing imaging core is equipped with an Hitachi TEM, an Hitachi SEM, an Olympus Fluoview 300 laser scanning confocal microscope, and two older model epifluorescence microscopes, one of which is equipped with a Spot II digital camera. While the electron microscopy facilities are state-of-the-art, the confocal and the fluorescence microscopy facilities had several limitations. First, the confocal microscope is equipped with only two channels and possesses a laser configuration which does not permit the use of dyes excited by near UV. The system is not upgradable and therefore investigators are effectively limited to dual label imaging with a restricted list of fluorophores. Second, the existing epifluorescence microscopes are inadequate for most immunofluorescence microscopy applications due to their older optical design. To overcome these limitations and to meet the needs of Projects 1, 3, and 5, the imaging core was augmented by the addition of 1) a Zeiss 510 Meta confocal microscope capable of eight channel imaging (seven fluorescent channels/one DIC channel) and detecting a wide range of fluorophores, 2) a confoCor2 FCS unit for fluorescence correlation spectroscopy, 3) an Axiovert200 microscope with a sensitive, fast digital camera (AxioCam HRM), and 4) a computer workstation with Zeiss software for processing and analyzing image data. These resources were required for three of the projects in the COBRE application. Their addition greatly enhances the research capabilities of investigators in the School of Medicine and the University of North Dakota.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 这里的目标是增加医学和健康科学学院中现有的成像核心设施，并增强北达科他大学的成像能力和能力。现有的成像核心配备了日立TEM，Hitachi SEM，Olympus Fluoview 300激光扫描共聚焦显微镜以及两个较旧的型号的落荧光显微镜，其中一个配备了Spot II数码相机。虽然电子显微镜设施是最先进的，但共焦和荧光显微镜设施有多个局限性。首先，共聚焦显微镜仅配备两个通道，并具有激光构型，该构型不允许使用近紫外线激发的染料。该系统无法升级，因此研究人员有效地限于具有荧光团列表的双重标记成像。其次，由于其较旧的光学设计，现有的落荧光显微镜对于大多数免疫荧光显微镜应用不足。为了克服这些局限性并满足项目1、3和5的需求，通过添加1）Zeiss 510 MetA共共性显微镜进行了增强，能够进行八个通道成像（七个荧光通道/一个DIC通道），并检测到频率2 fcs的荧光镜头3次频率分别，并检测到广泛的量子敏感，快速的数码相机（AxioCam HRM）和4）具有用于处理和分析图像数据的Zeiss软件的计算机工作站。这些资源是毛绒应用程序中的三个项目所需的。他们的补充大大提高了医学院和北达科他大学研究人员的研究能力。