EVALUATE CDK4/6 INHIBITOR PD0332991 ALONE/WITH RADIATION IN DIPG MOUSE MODEL

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 目标1. 评价PD 0332991治疗是否会延长携带由PDGF信号传导和Ink 4a-ARF丧失驱动的DIPG的小鼠的存活 目标2. 研究DIPG肿瘤细胞与PD 0332991的体内同步化是否可以增加放射治疗的疗效 在以前的机构中使用该模型时，我在治疗前仅使用T2加权成像对荷瘤小鼠进行成像。 然而，进行T1+对比研究将是有用的，因为我们也对研究血脑屏障（BBB）感兴趣，并且将来将产生BBB受损的荷瘤小鼠。 我建议我们对几只选择的小鼠进行T1+对比研究，这些小鼠在T2上有一个很好的肿瘤。 最终，该项目的数据将用于今年年底提交的R 01。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Aim 1. To evaluate whether treatment with PD0332991 will prolong the survival of mice harboring DIPG driven by PDGF signaling and loss of Ink4a-ARF Aim 2. To investigate whether in vivo synchronization of DIPG tumors cells with PD0332991 can increase the efficacy of radiation therapy When working with this model in a previous institution, I have only used T2 weighted imaging to image the tumor-bearing mice prior to treatment. However, it would be useful to carry out T1+contrast studies as we are also interested in studying the blood-brain-barrier (BBB) and in the future will be generating tumor-bearing mice with impaired BBB. I would suggest that we do T1+contrast studies on a couple of select mice with a nice size tumor on T2. Ultimately, data from this project will be used for an R01 submission at the end of this year.