DEVELOPMENT OF THE CALYX OF HELD

花萼的发育

• 批准号: 8361920
• 负责人: GEORGE A SPIROU
• 金额: $ 2.47万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-01 至 2012-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361920
• 关键词: Adult; Auditory; Brain; Brain Stem; Cell Nucleus; Communication; Development; Funding; Goals; Grant; Growth; Growth Cones; Hour; Image Analysis; Medial; National Center for Research Resources; Nerve; Neurons; Presynaptic Terminals; Principal Investigator; Process; Research; Research Infrastructure; Resources; Sensory; Source; Staging; Synapses; United States National Institutes of Health; cost; metaplastic cell transformation; nerve supply; neuronal cell body; postnatal; presynaptic; trapezoid body

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. We have previously established that growth of the calyx of Held, perhaps the largest synaptic terminal in the mammalian brain, occurs over a 48 hour period from postnatal day (P) 2 to P4. These terminals grow onto and cover large portions of the somata of principal neurons in medial nucleus of the trapezoid body (MNTB) in the auditory brainstem. Calyx growth requires tremendous cellular resources to achieve the morphological expansion of the terminals and the establishment of hundreds of active zones. During this process, only one of many converging presynaptic inputs will grow and form a calyx terminal, and thus rapidly establish 1:1 innervation that persists through adulthood, well before sensory input is detected and transmitted through this circuit. Our long-range goal is to reveal structural transformations and cellular communication that characterize contact of the calycigenic growth cone with its target and the early stages of synapse assembly and stabilization at large nerve terminals in the auditory brainstem. Our central hypothesis is that competition among calycigenic inputs precedes expansion of the terminal over the cell body to form a calyx.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 子项目的主要研究者可能是由其他来源提供的， 包括其他NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 我们以前已经建立了，生长的萼的赫尔德，也许是最大的突触终端在哺乳动物的大脑中，发生在48小时的时间从出生后的第2天（P）P4。这些终末长在听脑干斜方体内侧核（MNTB）的主神经元胞体上并覆盖其大部分。花萼的生长需要巨大的细胞资源来实现终端的形态扩展和数百个活动区的建立。在这个过程中，许多会聚的突触前输入中只有一个会生长并形成一个花萼末端，从而迅速建立1：1的神经支配，并持续到成年期，远在感觉输入被检测到并通过这个回路传输之前。 我们的长期目标是揭示结构转换和细胞通信的特点calycigenic生长锥与其目标和早期阶段的突触组装和稳定在听觉脑干中的大神经末梢的接触。我们的中心假设是，calycigenic输入之间的竞争之前，扩大终端的细胞体，形成一个萼。