DEVELOPMENT OF THE CALYX OF HELD

花萼的发育

• 批准号: 8361920
• 负责人: GEORGE A SPIROU
• 金额: $ 2.47万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-01 至 2012-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361920
• 关键词: Adult; Auditory; Brain; Brain Stem; Cell Nucleus; Communication; Development; Funding; Goals; Grant; Growth; Growth Cones; Hour; Image Analysis; Medial; National Center for Research Resources; Nerve; Neurons; Presynaptic Terminals; Principal Investigator; Process; Research; Research Infrastructure; Resources; Sensory; Source; Staging; Synapses; United States National Institutes of Health; cost; metaplastic cell transformation; nerve supply; neuronal cell body; postnatal; presynaptic; trapezoid body

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. We have previously established that growth of the calyx of Held, perhaps the largest synaptic terminal in the mammalian brain, occurs over a 48 hour period from postnatal day (P) 2 to P4. These terminals grow onto and cover large portions of the somata of principal neurons in medial nucleus of the trapezoid body (MNTB) in the auditory brainstem. Calyx growth requires tremendous cellular resources to achieve the morphological expansion of the terminals and the establishment of hundreds of active zones. During this process, only one of many converging presynaptic inputs will grow and form a calyx terminal, and thus rapidly establish 1:1 innervation that persists through adulthood, well before sensory input is detected and transmitted through this circuit. Our long-range goal is to reveal structural transformations and cellular communication that characterize contact of the calycigenic growth cone with its target and the early stages of synapse assembly and stabilization at large nerve terminals in the auditory brainstem. Our central hypothesis is that competition among calycigenic inputs precedes expansion of the terminal over the cell body to form a calyx.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 我们以前已经确定，从哺乳动物大脑中最大的持有花序的生长发生在产后（p）2到P4的48小时内发生。这些末端在听觉脑干中的梯形体（MNTB）的内侧核中生长并覆盖主要神经元的大部分somata。花萼生长需要巨大的细胞资源，以实现末端的形态扩展和建立数百个活跃区域。在此过程中，众多融合的突触前输入中只有一个会生长并形成花萼末端，从而迅速建立了1：1的神经，持续到成年期，良好，在检测到感觉输入并通过该电路传播之前。 我们的远距离目标是揭示结构转化和细胞通信，这些结构转换和细胞通信表征了钙化生长锥与其靶标的接触及其突触组装的早期阶段，并在听觉脑干中大神经终端的稳定阶段。我们的中心假设是，钙化输入之间的竞争是末端在细胞体上膨胀以形成花萼的竞争。